Vol. 36, Supplement A (2020)

Proceedings of the 67th Chemical Sensor Symposium
Nagoya, March 17-19, 2020

 

Abstracts



1.

Au系電極を用いたYSZセンサのVOC検知特性:
スピンコーティング法による薄膜検知極の作製と特性評価

長崎大院工

○上田 太郎、大井手 延光、鎌田 海、兵頭 健生、清水 康博

VOC-SENSING PROPERTIES OF YSZ-BASED GAS SENSORS ATTACHED
WITH Au-BASED ELECTRODES: PREPARATION OF THIN-FILM SENSING ELECTRODES
BY A SPIN-COATING METHOD AND EVALUATION OF THE SENSING PROPERTIES

Taro UEDA, Nobumitsu OIDE, Kai KAMADA, Takeo HYODO and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University
1-14 Bunkyo-machi, Nagasaki 852-8521

YSZ-based potentiometric gas sensors using a Au or CeO2-added Au sensing electrode (SE, CeO2 content: 4‒16 wt%, thickness: 30‒100 nm) were fabricated by a spin-coating method, and their sensing properties to toluene were examined at 500°C. The magnitude of response (ΔE) to 50 ppm toluene of the sensor attached with 4 wt% CeO2-added Au SE increased with a decrease in the SE thickness. On the other hand, ΔE of the sensors using 16 wt% CeO2-added Au SE increased with an increase in the SE thickness, and the sensor attached with the thickest 16 wt% CeO2-added Au SE showed the largest response (ca. 199 mV). This is probably due to an inreased number of the interfaces between CeO2-added Au and gas and the increased electrochemical oxidation of toluene at these sites.

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2.

h-BN補助電極を用いた起電力式水素センサの
応答特性に及ぼす湿式ミリングの効果

愛媛大学院理工

○板垣 吉晃、上田 裕司、猿丸 英理、青野 宏通

RESPONSE CHARACTERISTICS OF POTENTIOMETRIC HYDROGEN SENSOR
USING h-BN AUXILIARY ELECTRODE: EFFECT OF WET MILLING

Yoshiteru ITAGAKI, Yuji UEDA, Eri SARUMARU, Hiromichi AONO

Graduate School of Science and Engineering, Ehime University,
Matsuyama-shi, Ehime 790-857

Potentiometric sensors aiming at detecting H2 were examined. YSZ based sensor elements, H2, Pt/YSZ/Pt, air, responded to an ppm level of H2 contamination in air with the value of EMF=30 mV at 350oC. Coating the Pt sensing electrode with a powder of hexagonal boron nitride (h-BN) increased the EMF response, and the sensor response further increased by the milling treatment of the h-BN powder in ethanol. However milling treatment over 24 h reversed the response to decreasing. Specific surface area of the h-BN powder monotonously increased with the ball-milling which induced exfoliation of the BN layer. While, FT-IR analysis revealed that point defects existing in the BN plane react with solvent by the milling for > 48 h. It was concluded that the point defects in h-BN play a decisive role for the property of h-BN auxiliary phase in sensor response to H2.

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3.

混合伝導体を拡散層に用いた限界電流型酸素センサの酸素検知特性

九州大学大学院工学研究院1、九州大学カーボンニュートラル・ エネルギー国際研究所(I2CNER)2

○石原達己1,2,松本真都1

OXYGEN DETECTION PROPERTY OF LIMITTING-CURRENT TYPE OXYGEN SENSOR
USING MIXED CONDUCTOR FOR DIFFUSION LAYER

Tatsumi ISHIHARA,1,2 and Manato MATSUMOTO1

1Graduate School of Engineering, Department of Applied Chemustry, Kyusyu University,
Fukuoka-shi, Fukuoka 819-0395
2International Institute of Carbon Neutral Energy Research (I2CNER) Kyusyu University,
Fukuoka-shi, Fukuoka 819-0395

Limiting current type oxygen sensor is compact and detects in a wide oxygen concentration range. However, selectivity of this sensor is not high because almost all gases can be co-diffused in diffusion hole simultaneously with O2. The objective of this study is to develop limiting current type sensor with the high sensitivity and selectivity in wide oxygen partial pressure. The limiting current type sensor was prepared by using LaGaO3 based oxide for electrolyte as a fast ion conductor. For electrode, Co based oxide was mainly prepared by screen printing method. Instead of pin hole, mixed conductor of La(Sr)Ga(Fe)O3 which is stable hole and oxide ion mixed conductor was used for oxygen diffusion layer. Limmiting current was observed on I-V curves of the prepared sensor using LaGaO3 electrolyte at 600℃. Limiting current was linearly increased with increasing PO2 and so similar with conventional limiting current type sensor using hole layer, oxygen partial pressure was detect by limiting current. It was found that high selectivity to oxygen was achieved against H2, CO, and CH4 which shows high influence on detection in the conventional sensor.

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4.

c軸配向アパタイト型ランタンシリケート固体電解質を 用いた起電力式CO2センサ

九大院総理工a、三井金属鉱業b

馬 楠a、 井手 慎吾a b、 末松 昂一a、○渡邉 賢a、島ノ江 憲剛a

POTENTIOMETRIC CO2 SENSOR BASED ON c-AXIS ORIENTED
APATITE-TYPED LANTHANUM SILICATE

Nan MA a, Shingo IDE b c, Koichi SUEMATSU a, Ken WATANABE a, Kengo SHIMANOE a

a Faculty of Engineering Sciences, Kyushu University,
Kasuga, Fukuoka 816-8580, Japan
bEngineered Materials Sector R&D Center, Mitsui Mining & Smelting Co., LTD.,
Ageo, Saitama 362-0021, Japan cInterdisciplinary Graduate School of Engineering Sciences,
Kyushu University, Kasuga, Fukuoka 816-8580

In this study, we proposed a novel potentiometric CO2 gas sensor based on a new solid electrolyte of Y-doped La9.66Si5.3B0.7O26.14 (Y-LSBO) that show the high oxide ionic conductivity even at 300°C, attached with Li2CO3-CeO2-Au composite and Pt as sensing and reference electrode, respectively. As the results of optimizing the composition of sensing electrode, the EMF exhibits the linear relationship with the logarithm of CO2 concentration in the range of 400-4000 ppm at 400, 450 and 500°C. In addition, the optimized sensor exhibits an excellent Nernstian response to CO2 gas in not only dry but also humid atmospheres. These results strongly indicate that Y-LSBO-based solid electrolyte CO2 sensor is a promising candidate for CO2 monitoring with high sensor response and high stability.

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5.

単斜晶酸化タングステンによるNO2の低温・低濃度検知

熊本大工a、熊本大院先端b、熊本大院パルスc

〇坂尾 実希a、橋新 剛b、久保田 弘c

MONOCLINIC WO3 FOR LOW TEMPERATURE AND LOW CONCENTRATION DETECTION OF NO2

Miki SAKAO a, Takeshi HASHISHIN b, Hiroshi KUBOTA c

a Faculty of Engineering, Kumamoto University
Kumamoto-shi, Kumamoto 860-8555
b Faculty of Advanced Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
c Institute Pulse Power Science, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555

Tungsten oxide is a useful material for NO2 sensing. It has been reported that the crystalline phase of tungsten oxide provides gas selectivity. In this study, the NO2 sensing properties of monoclinic and hexagonal tungsten oxides prepared by hydrothermal synthesis were investigated. Their morphologie and crystal structure were investigated by field emission scanning electron microscope (FE-SEM), X-ray diffraction patterns (XRD). The NO2 gas sensing properties of monoclinic and hexagonal tungsten oxides were investigated at various operating temperatures from 50 to 200 ° C with 0.3 ppm NO2 concentration. As the result, at all temperatures, the sensor response of monoclinic tungsten oxide was higher than that of hexagonal tungsten oxide. The optimal operating temperature was 50 °C for monoclinic tungsten oxide and 100 °C for hexagonal tungsten oxide. The optimal operating temperatures of both sensors were lower than those of typical tungsten oxide sensors.

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6.

(La, Y)2O3/SnO2積層型センサのCO2応答に対する(La, Y)2O3層の表面被覆性改善の効果

豊橋技術科学大学a、富山県立大学b

○大野 賢a、 岩田達哉b、 高橋一浩a、 澤田和明a

INFLUENCES OF SURFACE COVERAGE OF A (La, Y)2O3 LAYER ON CO2 RESPONSE
IN (La, Y)2O3/SnO2 STACKED DEVICES

Ken ONO a, Tatsuya IWATA b, Kazuhiro TAKAHASHI a and Kazuaki SAWADA a

a Toyohashi University of Technology, Toyohashi-shi, Aichi 441-8580
b Toyama Prefectural University, Imizu-shi, Toyama 939-0398

In this study, the relationship between the CO2 response of (La, Y)2O3/SnO2 stacked devices and the thickness of sputtered (La, Y)2O3 films was investigated. The device had a 100-nm-thick SnO2 and a (La, Y)2O3 film with a thickness in the range of 15 – 90 nm. The CO2 responses both in dry and humid environments (20%RH) increased with increasing the (La, Y)2O3 thickness. Additionally, a larger response was observed in the device with a (La, Y)2O3 film deposited by sputtering followed by spin-coating deposition. This two-step deposition of the (La, Y)2O3 film may result in an increase in the surface area, leading to the response increase.

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7.

薄膜ガスセンサのpn接合形成による粒界障壁効果

熊本大院自然a、熊本大工b、熊本大院先端c、熊本大院パルスd

○奈良 康賢a、佐藤 宏b、坂口 知聡a、橋新 剛c,d、連川 貞弘c、久保田 弘d

POTENTIAL BARRIER EFFECT ON GRAIN BOUNDARY
BY PN JUNCTION IN THIN FILM GAS SENSORS

Yasumasa NARA a, Hiroshi SATO b, Chisato SAKAGUCHI a, Takeshi HASHISHIN c,d,
Sadahiro TSUREKAWA c, and Hiroshi KUBOTA d

a Graduate School of Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
b Faculty of Engineering, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
c Faculty of Advanced Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
d Institute Pulse Power Science, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555

Metal oxide semiconductor shows fairly good sensing performance to ppm concentration of various gases. It’s well-known that the formation of pn junction is very effective for improving their performance. This experimental evidence is based on the concept that the large amount of adsorbed oxygen is caused by the expansion of the depletion layer. However, nobody had succeeded to visualize the expansion of the depletion layer on oxide semiconductor thin film. In this study, the gas sensors with pn junction were fabricated by using vacuum evaporation and heat-treatment to investigate the pn junction effect on the sensor response to 3 ppm H2S. We also tried to visualize the depletion layer as the potential barrier using Kelvin Probe Force Microscope (KFM).

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8.

MgFe2O4 系 CO センサにおける水蒸気の影響

北九州高専

○小畑 賢次、松嶋 茂憲

INFLUENCES OF WATER VAPOR ON MgFe2O4 -BASED CO SENSOR

Kenji OBATA and Shigenori MATSUSHIMA

Department of Creative Engineering, National Institute of Technology (KOSEN),
Kitakyushu College, Shii 5-20-1, Kokuraminami-ku, Kitakyushu 802-0985

La-doped MgFe2O4 (MgFe2-xLaxO4) powders were prepared using a malic acid complex and their CO-sensing properties examined across a temperature range from 300 to 500 °C under humid conditions. The addition of La considerably enhanced the MgFe2O4 sensor’s CO sensitivity. Of the La-doped sensors investigated, the sensor with 7.5 mol% La-doped MgFe2O4 showed the highest sensitivity in the operating temperature between 300 and 350 °C. At 300 °C, the sensitivity to CO gas was estimated to be 11.2 times higher than that of the sensor fabricated using pure MgFe2O4 powder. At 350 °C, the electrical resistance of the MgFe1.85La0.15O4 sensor to 0 or 100 ppm CO increased with relative humidity (RH%). With constant humidity, the gas sensitivity (ΔR) of the MgFe1.85La0.15O4 sensor varied linearly with the logarithm of the CO concentration from 100 to 500 ppm range under humid conditions. For the MgFe1.85La0.15O4 sensor, gas sensitivity increased with relative humidity.

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9.

p-MgO/n-MgFe2O4接合界面での電位障壁高さ

熊本大院先端a、熊本大院自然b、熊本大院パルスc

○橋新 剛a,c、坂口 知聡b、奈良 康賢b、連川 貞弘a、松田 元秀a、久保田 弘c

POTENTIAL BARRIER HEIGHT AT THE INTERFACE OF p-MgO/n-MgFe2O4 JUNCTION

Takeshi HASHISHIN a , Chisato SAKAGUCHI b, Yasumasa NARA b,
Sadahiro TSUREKAWA a, Motohide MATSUDA a and Hiroshi KUBOTA c
a Faculty of Advanced Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
b Guraduate School of Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
c Institute Pulse Power Science, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555

It was clarified, in our previous studies, that MgO-Fe2O3 composite oxides showed fairly good sensor response to 10 ppb H2S, suggesting that MgO behaves as p-type and MgFe2O4 as n-type. The increase in the depletion layer with the formation of the p-n junction reflected the increase in sensor response. In this study, we prepared the core-shell spherical particles with Fe2O3 spherical particles as a core and MgO as a shell in order to measure those potential barrier heights. It was clarified by HAADF-STEM that the spherical particles had a hollow structure and microcrystals of MgFe2O4 were distributed at the particles from outside to inside. The lattice fringes in the microcrystal of HR-TEM image were identified as the (220) and (311) planes of MgFe2O4 within an error of 3%. It is considered that MgFe2O4 microcrystals were formed by diffusion and solid solution of Mg from the outside of spherical particles to the inside. From measuring the surface potential by KFM, not only the n-n junction by Fe2O3 and MgFe2O4 but also the p-n junction by MgFe2O4 and MgO were formed. Two junctions were appeared as the potential barrier height. It was suggested that MgO behaved as a semiconductor based on the temperature dependence of electrical resistance.

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10.

吸着燃焼式マイクロガスセンサ:触媒担体の組成がVOC 検知特性に及ぼす効果

長崎大院工a、矢崎エナジーシステムb

○兵頭 健生a、松浦 侑馬a、笹原 隆彦b、 鎌田 海a、上田 太郎a、清水 康博a

ADSORPTION/COMBUSTION-TYPE GAS SENSORS: EFFECTS OF COMPOSITION OF
CATALYTIC SUPPORTS ON VOC-SENSING PROPERTIES

Takeo HYODOa, Yuma MATSUURAa, Takahiko SASAHARAb,
Kai KAMADAa, Taro UEDAa, and Yasuhiro SHIMIZUa

aGraduate School of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
bGas Equipment R&D Center, Yazaki Energy System Corporation,
23 Minamikajama, Shizuoka 431-3393, Japan

Sensing properties of adsorption/combustion-type gas sensors utilizing 5 wt% Pt-loaded alumina (5Pt/γ(r)α(t)-Al2O3 sensors, r and t: the mixing ratio of γ-Al2O3 and α-Al2O3 as a catalytic support (wt%)) to volatile organic compounds (VOCs, ethanol and toluene) have been investigated in this study. The magnitude of general responses and the integrated dynamic responses of the 5Pt/γ(50)α(50)-Al2O3 sensor were the largest among the series of sensors, which were probably affected by the adsorption property and the oxidation activity of ethanol on their surface and their thermal conductance. On the other hand, their integrated dynamic response gradually increased with an increase in the amount of α-Al2O3, probably because their improved thermal conductance and oxidation activities per unit surface area. We will discuss their sensing properties to ethanol as well as toluene in the symposium, on the basis of further detailed results.

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11.

二種類のNOx分析チップによる呼気中NO測定法の検討

東北工大

〇浅沼 光吾、 沼田 佳大、 丸尾 容子

MEASUREMENT OF NITRIC OXIDE PRESENT IN A EXHALED AIR
USING TWO TYPES OF NOx ANALYTICAL CHIPS

Kohgo ASANUMA, Keita NUMATA, Yasuko Y. MARUO

Tohoku Institute of Technology, Sendai-shi, Miyagi 982-8577

Nitric oxide (NO) contained in human breath has a correlation with the extent of asthma, and clinical tests using exhaled breath have attracted attention in the medical field. We developed an analytical chip for detection NO composed of a porous glass and a 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxyde-1-oxyl (PTIO). In this study, to measure the NO concentration in exhaled air with high sensitivity, we devised a measurement method using two types of analytical chips. PTIO chip converted NO into NO2, which was found to be 1:1 relationship. Therefore, we devised a method of measuring the converted NO2 using a NO2 analytical chip, and two types of chips were simultaneously exposed to the NO atmosphere. The percentage of converted NO gradually increased with time, and that was 93% ± 2% and 99% ± 1% at 12 hours and 24 hours, respectively. There was a linear relationship between the logarithmic change in absorbance at 567 nm of the PTIO chip and exposure time, but the accuracy and sensitivity were found to be low. For the developed method using NO2 analytical chip, high correlation was obtained between the amount of change in absorbance at 525 nm and the exposed NO concentration. These results suggested that the method using the two types of chips enables high sensitivity measurement of NO in exhaled air.

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Plenary Lecture

INTELLIGENT GAS SENSING SYSTEM AND ITS APPLICATIONS

○Da-Jeng YAO

Department of Power Mechanical Engineering, Institute of NanoEngineering and MicroSystems,
and Department of Engineering and System Science, National Tsing Hua University,
101, Sec. 2, Kuang-Fu road, Hsinchu 30013, Taiwan

This presentation will first introduce why we need the E-nose and how to build the intelligent gas sensing array system based on both CMOS-based resistive type and surface acoustic wave (SAW) type sensing mechanisms. Furthermore, several applications would be developed based on the developed gas sensing system. A plug-in handheld smart gas sensing device for freshness of food and other safety; Beverage manufacture and quality detection including tea, coffee bean, and red wine, etc.; Indoor or outdoor environmental gas sensing system for PM 2.5, second hand smoke detection, and other hazardous gas; Medical diagnostic applications; and other IOT applications, etc.

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Keynote Lecture

ROOM-TEMPERATURE OPERATED GRAPHENE-BASED
GAS SENSING MEMBRANE

○Chia-Ming YANGa,b,c, Tsung-Cheng CHENb

aInstitute of Electro-Optical Engineering, Chang Gung University, Taoyuan, Taiwan,
bDepartment of Electronic Engineering, Chang-Gung University, Taoyuan 333, Taiwan
cDepartment of General Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan

In conventional gas sensing material including metal oxide, the high-temperature operation (>200 oC) is necessary for response and recovery, which has a drawback of power consumption. Graphene with superior properties including high surface-to-volume ratio and high conductivities can be a good candidate of the gas sensing material. The most attractive behavior of graphene-based gas sensor is the room-temperature operation. We already demonstrated the acetone sensing of 600 ppb of 1.9% response at room temperature. Due to the good adsorption of gas molecular on graphene, the response and recovery can be further improved by UV illumination for a long-term usage. With a short-time UV illumination of 5 min, the response can be 7-fold (7X) higher and recovery can be fully returned to baseline with a very low power consumption of UV LED. We also observed the spacing of interdigital electrodes from 50 to 400 μm can help on the acetone sensitivity for 4X higher, which may be from the doping effect by glass substrate. Furthermore, a rapid thermal annealing treated on graphene can also help for approximately 4X in NO2 sensing response with more than 95% recovery by UV illumination.

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清山賞受賞講演1

ビーズ型半導体式ガスセンサの駆動温度による検知機構の開発

NISSHAエフアイエス株式会社

○栗林 晴美

DEVELOPMENT OF DETECTION MECHANISM DEVISED ON DRIVING TEMPERATURE
OF BEAD TYPE SEMICONDUCTOR GAS SENSOR

Harumi KURIBAYASHI

Nissha FIS, INC.,
2-4-28 Tagawa, Yodogawa-ku, Osaka 532-0027

The bead-type semiconductor gas sensors sold by our company are characterized by excellent thermal responsiveness. Based on this characteristic, there sensors periodically change the element temperature, detect methane at high temperatures, and detect CO at low temperatures. By processing and analyzing the high and low temperature sensor signals obtained by this drive or a slightly different drive to which this drive was applied, we have developed additional technologies that can supplement and improve gas identification, detection accuracy, and durability of sensor elements, which were disadvantages of previous gas sensors. This technology is expected to be applied not only to bead-type semiconductor gas sensors, but also to small sensors such as MEMS sensors.

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清山賞受賞講演2

表面プラズモン共鳴センサを用いた匂い分子の超高感度検出に関する研究開発

九大院シ情

○小野寺 武

RESEARCH AND DEVELOPMENT OF SURFACE PLASMON RESONANCE SENSOR
FOR ULTRA-HIGHLY SENSITIVE DETECTION OF ODOR SUBSTANCES

Takeshi ONODERA

Faculty of Information Science and Electrical Engineering, Kyushu University,
744 Motooka, Fukuoka 819-0395, Japan

This report describes the research and development of a surface plasmon resonance sensor which detects specific odor substances with ultra-high sensitivity by combining an SPR sensor as a transducer and antibodies to recognize odor molecules. Specifically, the preparation of unique antibodies against low molecular weight substances such as trinitrotoluene and benzaldehyde, the preparation of sensor surfaces which suppresses nonspecific adsorption, the development of sampling methods and measurement protocols, the results of demonstration experiments, and examples of practical applications are explained.

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12.

安定した電位計測を目指した 電気化学汗成分センサの開発

山形大有機材料シa、山形大ROELb、山形大工c、東京理科大d

○長峯 邦明a,b、市村 祐介a、栗坪 巧c、井澤 良太a
松井 弘之a,b、 四反田 功d、時任 静士a,b

A POTENTIOMETRIC CHLORIDE ION SENSOR EMPLOYING A HYDROGEL-BASED TOUCH PAD
FOR NON-INVASIVE SWEAT ELECTROLYTE ANALYSIS

Kuniaki NAGAMINEa,b, Yusuke ICHIMURAa, Takumi KURITSUBOc, Ryota IZAWAa,
Hiroyuki MATSUIa,b, Isao SHITANDAd, and Shizuo TOKITOa,b

a Graduate School of Organic Materials Science, Yamagata University,
Yonezawa, Yamagata 992-8510
b Research Center for Organic Electronics (ROEL), Yamagata University,
Yonezawa, Yamagata 992-8510
c Faculty of Engineering, Yamagata University,
Yonezawa, Yamagata 992-8510
d Department of Pure and Applied Chemistry, Faculty of Science and Technology,
Tokyo University of Science, Noda, Chiba 278-8510

We have recently proposed and demonstrated passive, non-invasive extraction and in-situ potentiometric detection of human sweat chloride ions (Cl- ions) using a hydrogel-based touch-sensor pad. The sensor pad was composed entirely of a screen-printed bare Ag/AgCl-based chloride ion-selective electrode and a planar liquid-junction Ag/AgCl reference electrode, which were fully covered by an agarose hydrogel in phosphate-buffered saline. When human skin contacted to the hydrogel pad, sweat Cl- ions were continuously extracted into the gel, followed by in situ potentiometric detection. The planar liquid-junction Ag/AgCl reference electrode had a polymer-based KCl-saturated inner electrolyte layer to stabilize the potential of the Ag/AgCl electrode even with a substantial change of the chloride ion concentration in the hydrogel pad. We expect this fully screen-printed sensor to achieve low-cost passive and non-invasive daily monitoring of human chloride ions in sweat without activity such as exercise for inducing perspiration, eliminating the need for environmental temperature control, or requiring a cholinergic drug administration.

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13.

ポリチオフェン複合体の固体発光能評価とフィルム型化学センサへの展開

産総研a、筑波大院数理b 

○江坂亮祐a,b、南木 創a、栗田僚二a,b

SOLID-STATE EMISSION OF A POLYTHIOPHENE COMPOSITE AND ITS APPLICATION
FOR A FILM-BASED CHEMICAL SENSOR

Ryosuke ESAKAa,b, Tsukuru MINAMIKIa and Ryoji KURITAa,b

a Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST),
Tsukuba, Ibaraki 305-8566
b Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8577

Significant attention is being devoted to the development of film-based chemical sensors for the visualization of chemical information in environments and our bodies. However, quantitativity and sensitivity of conventional film-based colorimetric sensors utilizing dyes or nanoparticles are much lower than liquid types of chemical sensors. Therefore, the preparation of film-based sensors using fluorescence materials is desired to broaden the application fields of film-based sensors. Although π-conjugated polymers including polythiophenes (PTs) are some of the best platform materials for the fabrication of chemical sensors owing to their multivalent molecular recognition ability (=high-sensitivity for analytes) and film formability, it is generally hard to establish both functions of solid-state emission and chemical sensing. Herein, we propose a novel strategy for the simple preparation of a film-based sensor using the PT. The proposed fluorescence assay on the film has been achieved by only using the mixture of a carboxy-attached PT and an amine-terminated PEG copolymer. Our successful demonstration of the spontaneous construction method of the film-based fluorescence sensor could lead to the development of easy usable on-site chemical sensors with high quantitativity and sensitivity.

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14.

ペロブスカイト型酸化物担持電極の 亜硝酸イオン応答特性

九工大院工

○森山実加子、高瀬 聡子、清水 陽一

NITRITE-ION RESPONSE CHARACTERISTICS OF PEROVSKITE-TYPE
OXIDE SUPPORTED ELECTRODES

Mikako MORIYAMA, Satoko TAKASE and Youichi SHIMIZU

Department of Applied Chemistry, Kyushu Institute of Technology,
1-1 Sensui-cho, Tobata, City of Kitakyushu, Fukuoka 804-8550

Detection of nitrite ions is important in various industrial fields such as food additives and rust inhibitors for steels. In study of the perovskite-type oxide supported electrodes were studied as current detection type nitrite ion sensors. The oxidation current of the SmFeO3 supported electrode increasing with increasing a concentration of nitrite ion. To improve of sensing properties, the effect of electrode materials and pH condition were investigated. The response characteristics of carbon and gold electrodes with SmFeO3 which were prepared by electrophoretic deposition (EPD), were evaluated. It was found that both electrodes supporting SmFeO3 exhibited high selectivity and a good linear response to nitrite ions over a wide range of concentrations. The detection range of the SmFeO3 / GC electrode was 10 μM – 5000 μM with the sensitivity having 33.1 mA M-1 cm-2 (+0.85 V vs Ag / AgCl).

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15.

細胞の呼吸活性測定のためのマイクロデバイスとその応用

筑波大院数理物質a、防衛医大b

○薛 安汝a、 佐藤 達哉a、 朴 善浩a、木下 学b、 鈴木 博章a

MICRODEVICE FOR MONITORING OF THE RESPIRATORY ACTIVIY
OF CELLS AND ITS APPLICATION

An-Ju HSUEH a, Tatsuya SATO a, Sunho PARK a,
Manabu KINOSHITA b and Hiroaki SUZUKI a

a Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba-shi, Ibaraki 305-8573
b National Defense Medical College,
Tokorozawa-shi, Saitama 359-8513

There are increasing demands to measure the respiratory activity of animal and microbial cells. To this end, a miniaturized Clark-type oxygen electrode is useful. To facilitate handling by end-users, we used a structure containing a dried electrolyte sealed by a hydrophobic oxygen-permeable membrane and water necessary for the reduction of oxygen was introduced there in the form of water vapor through the oxygen-permeable membrane. The device was used to examine the efficacy of an antibiotics (oxacillin). Changes in the respiratory activity of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) was monitored. Clear differences were observed between MSSA and MRSA and depending on the concentration of the antibiotics, demonstrating that the device can be used to evaluate the efficacy of antibiotics.

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16.

バイポーラ電気化学顕微鏡システムの開発と
高時空間分解能バイオイメージング応用の検討

東北大学 環境科学研究科a,学際科学フロンティア研究所b
材料科学高等研究所c,イノベーション戦略推進センターd

○岩間智紀a,井上(安田)久美a,阿部博弥b,c,末永智一d,珠玖 仁a

DEVELOPMENT OF THE BIPOLAR ELECTROCHEMICAL MICROSCOPY AND ITS DEMONSTRATION
FOR HIGH SPATIO-TEMPORAL RESOLUTION BIO-IMAGING APPLICATION

Tomoki IWAMA a, Kumi Y. INOUE a, Hiroya ABE b,c, Tomokazu MATSUEd and Hitoshi SHIKUa

a Graduate School of Environmental Studies, Tohoku University,
Sendai-shi, Miyagi 980-8579
b Frontier Research Institute for Interdisciplinary Sciences, Tohoku University,
Sendai-shi, Miyagi 980-8578
c Advanced Institute for Materials Research, Tohoku University,
Sendai-shi, Miyagi 980-8577
d Center for Promotion of Innovation Strategy,
Sendai-shi, Miyagi 980-0845

An electrochemical imaging with a multi electrode array has been applied for various bio-sample observations such as a metabolic activity of cell spheroids, and a neuro-transmitter release activity in a rat brain slice. For these observations in a small area of a biological sample, a higher density electrode array is required. However, conventional arrays require the presence of connecting lines between the voltage supplier and individual electrodes, which severely limits the resolution of the resulting images. To overcome this problem, we have employed a closed bipolar electrode (cBPE) with electrochemiluminescence (ECL) detecting system as a new platform for bioimaging. In this research, we demonstrated the imaging of a respiration activity of the MCF-7 spheroid by using a cBPE array fabricated by depositing gold electrode in a micropore membrane. The ECL that de-rived from oxidation of [Ru(bpy)2]Cl2 and tripropylamine was used as the signal for imaging. A living spheroid and a dead spheroid, immerged in paraformaldehyde, was prepared and put on the cBPE array. As a result of applying a driving voltage, ECL signals at the position where the living spheroid was placed significantly decreased. This result indicates that this system can be applied for the observation of biological activities.

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17.

混成単分子膜を導入した FET型化学センサにおける分子認識能の系統的調査

産総研 a、筑波大院数理 b

〇南木 創 a、市川佑貴 a,b、栗田僚二 a,b

SYSTEMATIC INVESTIGATION OF MOLECULAR RECOGNITION ABILITY IN FET-BASED
CHEMICAL SENSORS MODIFIED WITH MIXED SELF-ASSEMBLED MONOLAYERS

Tsukuru MINAMIKIa, Yuki ICHIKAWAa,b and Ryoji KURITAa,b

a Biomedical Research Institute,
National Institute of Advanced Industrial Science and Technology (AIST),
Tsukuba, Ibaraki 305-8566
b Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8577

Exploring novel strategies for simple methods of tuning the sensing ability of sensor devices modified with molecular recognition materials is important to realizing high-throughput on-site sensing systems based on integrated and miniaturized devices. For instance, although field-effect transistor (FET)-based chemical sensors can be used for rapid, quantitative, and simultaneous determination of various desired analytes, detectable targets in conventional FET sensors are currently restricted owing to the complicated processes used to prepare sensing materials. In this study, we investigated the relationship between the sensing features of FETs and the nanostructures of mixed self-assembled monolayers (mSAMs) for the detection of biomolecules. The FET devices were systematically functionalized using mixtures of benzenethiol derivatives (4-mercaptobenzoic acid and benzenethiol), which changed the nanostructure of the SAMs formed on gold sensing electrodes. The obtained cross-reactivity in the FETs modified with the mSAMs was derived from the multidimensional variations of the SAM characteristics. Our successful demonstration of continuous control of the molecular recognition ability in the FETs by applying the mSAM system could lead to the development of next-generation versatile analyzers, including chemical sensor arrays for the determination of multiple analytes.

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18.

電気化学免疫センサの開発

北大国際食資源学院a、北大国際連携研究教育局b

○川口俊一a,b、佐藤陽亮a、高橋昌志a,b

DEVELOPMENT OF ELECTROCHEMICAL IMMUNOSENSOR

Toshikazu KAWAGUCHIa,b, Yosuke SATOa , Masashi TAKAHASHIa,b

a Graduate School of Global Food Resources, Hokkaido University,
Sapporo-shi, Hokkaido 060-0809
b Global Institution for Collaborative Research and Education, Hokkaido University,
Sapporo-shi, Hokkaido 060-0808

Electrochemical immunosensor is proposed in this research. This is combination of electrochemical sensing and immunoreaction. In immunoreaction, the size effect was used for sensing. However, a size of a regular antibody was too large (approximately 12 nm) to detect a small analyte. Therefore, only the bio-recognition element was newly synthesized by bacillus with the enzyme. As a result, the recognition element became enough small to detect a protein (approximately 14 nm). It is named as VHH antibody. Planar-typed electrochemical chip was newly designed and fabricated. This chip has four Au electrodes on the surface. Counter electrode and the reference electrode are Au electrode and polyaniline modified Au electrode, respectively. Working electrode is mixed monolayer of VHH antibody-propanethiol/Au, ferrocenyl-propanethiol/Au, and ethanolic-propanethiol/Au. The electrochemical immunosensor chip was rinsed with PBS solution immediately after contacting with OVA standard solution. And then, cyclic voltammograms have been measured in 0.1 M NaCl. The oxidation peak of ferrocene was observed, but it was very small. Therefore, ferricyanide was added to electrolyte solution. The promotion effect of ferrocene and ferricyanide improved the oxidation peak. It was found that two types of calibration plot depicted different detectable range. The calibration plot of oxidation peak potential with respect to the OVA concentration showed wide detectable range from 1 ppt to 10 ppm. Another plot of the anodic current at +0.35 V illustrated that the OVA of 0 to 10 ppt could be very sensitively detected. In past, ELISA and the direct immunoassay format by using Surface Plasmon Resonance (SPR) Immunosensor showed the lowest detection limits were reported as 1 ppb to 10 ppb. This electrochemical immunosensor showed the comparable lowest detection limit with indirect competitive inhibition by using SPR.

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19.

分子インプリント高分子のナノ粒子の膨潤挙動からバンコマイシンセンサの測定原理の考察

芝浦工大

○吉見 靖男、阿部 柾樹、武田 悠利、江口 晴登

SPECULATION OF MECHANISM OF THE VANCOMYCIN SENSOR BY SWELLING PHENOMENA
 OF VANCOMYCIN-IMPRINTED POLYMER NANOPARTICLES

Yasuo YOSHIMI, Masaki ABE, Yuuto TAKEDA, Haruto EGUCHI

Department of Applied Chemistry, Shibaura Institute of Technology Koto-ku,
Tokyo 135-8548

We have reported that the paste electrode of the graphite particle on which vancomycin-imprinted polymer is grafted generates current which depends on vancomycin concentration. The phenomenon is feasible for sensor which detects vancomycin in blood. However, the mechanism is still in a black box. To understand the mechanism, the dependency of the water-contact angle at the surface of the carbon paste electrode imprinted by vancomycin on the vancomycin concentration was observed. The result indicates that the surface of the imprinted electrode is hydrophilized by the specific interaction with vancomycin. The nanoparticle of vancomycin imprinted polymer is swelled by the specific interaction with the specific interaction. However, those results have inconsistency in the selectivity, thus it is unlikely that the swelling is important factor of the present sensing principle of the paste electrode grafted with the MIP of VCM.

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20.

表面プラズモン共鳴チップ上での特徴パターン認識による培養細胞評価

産総研バイオメディカルa、筑波大院数理物質b

○栗田 僚二a,b、 菅井祥加a、冨田 峻介a、石原 紗綾夏a、吉岡 恭子a

CELL CULTURE ASSESSMENT BY FINGERPRINTING ARRAY
ON A SURFACE PLASMON RESONANCE CHIP

Ryoji Kurita a,b , Hiroka Sugai a, Shunsuke Tomita a, Sayaka Ishihara a, Kyoko Yoshioka a

a National Institute of Advanced Industrial Science and Technology,
1-1-1 Higashi, Tsukuba, Ibaraki 305-8566,
b University of Tsukuba,
1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577

We present the pattern-based cell characterization utilizing a cysteine derivatives-immobilized multichannel surface plasmon resonance (SPR) chip. Five gold films with different cysteine derivatives were integrated onto the chip in a microchannel. The cysteine derivatives were capable of generating the SPR response patterns that were unique to the components of cell culture media through diverse cross-reactive interactions, allowing the identification of cell types by multivariate analysis. The chip provides the SPR response patterns that reflect states and activities of drug-treated cells, enabling a noninvasive cytotoxicity assay. Our chip can easily offer the complicated information that is elucidated by the combination of multiple assays in general by just one injection with a small amount of cell culture media (~ 25 μL) into the chip and rapid sensing (~ 10 min). Our approach could potentially be used as a versatile tool for the characterizing cells.

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21.

四重極電極アレイを用いた
イオノフォア刺激による細胞電気特性変化の評価

兵庫県大院物質理a、パナソニック株式会社b

○河合 志希保 a,b、 鈴木 雅登a、 有本 聡b、 是永 継博b、 安川 智之a

MONITORING OF ELECTROROTATION RATES OF CELLS
BY THE QUADRUPOLE ELECTRODE ARRAY UPON THE IONOPHORE STIMULATION

Shikiho KAWAI a,b , Masato SUZUKI a , Satoshi ARIMOTO b ,
Tsuguhiro KORENAGA b and Tomoyuki YASUKAWA a

a Graduate school of material science, University of Hyogo,
3-2-1, Kouto, Kamigori, Ako, Hyogo 678-1298
b Technology Innovation Division, Panasonic Corporation,
3-1-1, Yagumo-naka-machi, Moriguchi, Osaka 570-8501

In this presentation, we monitored electrorotation rates of cells upon the ionophore stimulation to estimate the variation of electric characteristics of cells. Recently, three-dimensional electrode array was developed to rotate cells simultaneously. However, the addition of chemical stimuli is impossible because of the flow of the solution in the microchannel. Thus, we developed new device with quadruple electrode array integrated on the bottom substrates. When AC voltage with 90 degree differences of phase was applied to each electrode, cells rotated at grids formed by quadruple electrodes simultaneously. In the presence of Ca2+ in the solution for the electrorotation, the rotation rates of cells decreased after adding the solution containing ionomycin, while fluorescence intensity increased. However, no obvious variations for the rate and the intensity were observed in the absence of Ca2+. Therefore, the decrease of the membrane potential by the influx of Ca2+ permeated through the ionomycin would lead to the decrease of the electrorotation rates. We can monitor the electrorotation rates of cells upon the chemical stimuli.

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22.

ELECTROCHEMICAL MONITORING OF VIABILITY OF YEAST CELLS
BY USING A NEW DOUBLE MEDIATOR SYSTEM AND
SENSING OF ANTIFUNGAL AGENT

○Mohammad Abdul ALIM1,2, Hiroaki SHINOHARA1, Minoru SUGA1

1Graduate School of Innovative life Science, University of Toyama,
Toyama-shi, Toyama 930-8555, Japan
2Bangabandhu Sheikh Mujibur Rahman Science and Technology University Gopalganj,
8100, Bangladesh

We have previously developed the electrochemical method for evaluation of the mammalian cell viability, cell counting and cytotoxicity of drugs by monitoring intracellular NADH concentration with a new double mediator system coupled 1-mPMS with [Fe(CN)6] 3- on a screen-printed carbon electrode (SPCE). It was demonstrated that the method was rapid and wider concentration of cell detectable as compared with conventional colorimetric method. In this study, we next applied our electrochemical method to monitor the intracellular NADH of fission yeast cells for the evaluation of yeast cell viability and accurate cell counting. The oxidation current in the amperometric measurements at 500 mV vs. Ag/AgCl showed good linearity to the number of Schizosaccharomyces pombe cells. Furthermore, the inhibition effect of some antifungal agents to yeast cells has been tested by our method and it will be mentioned at the time of presentation.

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特別講演1

CD型マイクロ流路デバイスによるバイオセンシング

創価大理工

○久保いづみ

BIOSENSING UTILIZING CD-SHAPED MICROFLUIDIC DEVICE

Izumi KUBO

Soka University Hachioji,
Tokyo, 192-8577

The application of compact disc (CD)-shaped microfluidic devices to biological sensing has been investigated. On these devices, solutions including samples are introduced and transported into microchannels by centrifugal force without using any pump and where micro analyses can be conducted. These powerful aspects of such devices have enabled them to be used for simple and high-throughput analyses. We have developed CD-shaped microfluidic devices that can be used as a biosensor for various applications. Three applications are reported here: to single cell isolation and detection of a cell-specific gene, discrimination of an entrapped cell by staining, and high-throughput analyses based on the enzyme linked immunosorbent assay (ELISA). In this chapter, we summarize the applications of biosensors performed on CD-shaped microfluidic devices such as gene analysis of isolated cells based on a specialized polymerase chain reaction (PCR), cell staining for nonadherent cells, and rapid ELISA.

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特別講演2

ストレス計測評価用バイオセンシングデバイスの研究開発

産総研・阪大先端フォトバイオラボa、神大院人間発達b、阪大院工c

○脇田 慎一a,b,c

R & D OF BIOSENSING DEVICES FOR STRESS MONITORING

Shin-ichi WAKIDAa,b,c

a AIST PhotoBIO-OIL, National Institute of Advanced Industrial Science and Technology (AIST),
2-1 Yamada-Oka, Suita, Osaka 565-0871
b Graduate School of Human Development and Environment, Kobe University
3-11 Tsurukabuto, Nada-ku, Kobe, Hyogo 657-8501
c Graduate School of Engineer, Osaka University,
2-1 Yamada-Oka, Suita, Osaka 565-0871

Stress is defined as the adaptive defense response against changes in the environment as the stress theory. The stress might be evaluated by measuring neurotransmitters in the brain and blood stress hormones. The invasive sampling becomes a very large stress stimuli, and therefore, accurate stress cannot be evaluated especially for healthy persons. Saliva has been attracting attention as an alternative blood sample. The science of saliva secretion mechanisms for the stress defense response as well as the R&D on measurement tool can be visualized for the stress biological response. Using advanced biosensor & biochip technologies, we have made several R&D on single drop analysis for saliva stress marker candidates for ergonomics and clinical research field for 20 years. Here, we will introduce research strategy and results on some topics among the R&D.

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23.

耐熱性グルコースデヒドロゲナーゼ
固定化電極の応答特性

埼玉工大・工

○長谷部 靖、 竹内 隆裕、 榎本 紗南

RESPONSE CHARACTERISTICS OF THERMOSTABLE GLUCOSE
DEHYDROGENASE-IMMOBILIZED ELECTRODE

Yasushi HASEBE, Takahiro TAKEUCHI and Sana ENOMOTO

Department of Life Science and Green Chemistry, Saitama Institute of Technology,
1690 Fusaiji, Fukaya, Saitama 369-0293

Thermostable glucose-1-dehydrogenase (tGDH) was immobilized onto the activated carbon paste electrode (CPE) to fabricate glucose-sensing electrode. Enzymatically produced NADPH from NADP+ during the tGDH-catalyzed glucose oxidation was electrochemically oxidized at the CPE. Pre-activation of the CPE by organic dye and heat treatment was significantly important to obtain larger catalytic current response. In the presence of glucose, tGDH-modified CPE (tGDH-CPE) exhibited two separated oxidation currents on cyclic voltammogram (CV) and differential pulse voltammogram (DPV). The separated DPV peaks increased with increase in the glucose concentration (from 0.1 to 100 mM) at pH 6.5 and 35ºC. The calibration curve of peak A (observed at 0 V) has the linear range at 1-decade lower concentration region as compared with that of peak B (observed at 0.4 V). This glucose sensor works at mild temperature (25-35ºC), although the optimal temperature of native un-immobilized tGDH is 75-95ºC.

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24.

CVD二層グラフェン膜を用いた
バイオフィルム形成-成長のセンシング

鈴鹿高専a,鈴鹿医科大b, 産総研c, 日工大d, Clarkson Univ./SUNY Cantone

〇中川遼一a,斉藤 開a,三浦英和b, 石原正統c, 伴 雅人d,
平井信充a, 幸後 健a, 小川亜希子a, バリー ダナe

SENSING FOR FORMATION AND GROWTH OF BIOFILMS
BY TWO LAYERED CVD GRAPHENE FILMS

Hideyuki KANEMATSUa, Ryoichi NAKAGAWAa, KAI SAITOa,
Hidekazu MIURAb,Masato ISHIHARAc, Masato BANd,
Nobumitsu HIRAIa, Takeshi KOGOa, Akiko OGAWAa, Dana M. BARRYe

aNIT (KOSEN), Suzuka College,shiroko-cho, Suzuka, Mie 510-0294
bSuzuka Univ. Med.Sci., 1001-1, Kishioka-cho, Suzuka, Mie 510-0293
cAIST, 1-1-1, Umezono, Tsukuba, Ibaraki, 305-8560
dNippon Inst.Tec., 4-1,Gakuendai, Miyashiro-cho,
Minami Saitama-gun, Saitama, 345-8501
eClarkson Univ./SUNY Canton, Potsdam, NY13699 / 34 Cornell Drive,
Canton NY13617, the USA

Biofilms are inhomogeneous thin film like matters formed on materials’ surfaces by bacterial activities. They are composed of water (more than 80%), extracellular polysaccharide (EPS) and bacteria themselves. Once they form on material parts of the structures, they might lead to the formation of scales within pipes, corrosion, the deterioration in healthy conditions, induced infection and chronic diseases, etc. They have positive applications to energy, environmental problems on the other hand. At any rate, they should be controlled properly. To achieve the purpose, we needed a suitable material sensitive to biofilm formation and growth. In our previous studies, we found that graphene was very sensitive to biofilms particularly among sensitive carbon materials. We formed two layered graphene on pure copper foils by a CVD process. Then the specimens were put in wells filled with bacterial cultures in a certain time to produce biofilms on graphene specimens. The impedance of graphene specimens was measured by a LCR meter, while the specimens’ surfaces were analyzed by a Raman spectroscopy. Comparing those results, we found some peaks appeared at some frequencies for the specimens forming biofilms. We discussed the application possibility to a biofilm sensor in the future.

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25.

有機分子触媒を利用したバンコマイシンの電気化学検出

東北大院薬a、奥羽大薬b

○佐藤勝彦a、小野哲也b、吉田健太郎b、大樂武範b、柏木良友b

ELECTROCHEMICAL DETECTION OF VANCOMYCIN USING AN ORGANOCATALYSTS

Katsuhiko SATO a, Tetsuya ONO b, Kentaro YOSHIDA b,
Takenori DAIRAKU b and Yoshitomo KASHIWAGI b

a Graduate School of Pharmaceutical Sciences, Tohoku University,
6-3 Aoba, Aramaki, Aoba-ku, Sendai 980-8578
b School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido,
Tomita-machi, Koriyama, Fukushima 963-8611

This work investigated a electrochemical detection of vancomycin (VCM) using organic electron transfer catalyst such as 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO) and Nortropine-N-oxyl (NNO). NNO is a highly active nitroxyl radical compound. However, NNO was unable to detect VCM because NNO was inactivated by primary amines of VCM in aqueous solution. On the other hand, 4-acetoamide-TEMPO (A-TEMPO) showed increase anodic current in the presence of VCM, which indicates that A-TEMPO able to catalyze the oxidation of hydroxy groups in VCM under neutral aqueous conditions at 25 °C. A linear response to the VCM concentration ranging from 10 to 100 μM was obtained. We synthesized TEMPO precursor containing phenol side chain. This research describes the voltammetric behavior of TEMPO-modified GC electrode prepared by the electrochemical polymerization of this TEMPO derivative.

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26.

人工シデロフォア修飾基板を用いた微生物センシング

名工大院工

○猪股 智彦、 遠藤 卓、 宮本 政和、 小澤 智宏、 増田 秀樹

SENSING MICROORGANISMS USING ARTIFICIAL
SIDEROPHORE-MODIFIED SUBSTRATES

Tomohiko INOMATA, Suguru ENDO, Masakazu MIYAMOTO,
Tomohiro OZAWA, and Hideki MASUDA

Graduate School of Engineering, Nagoya Institute of Technology,
Nagoya-shi, Aichi 466-8555

Siderophores are small chelating molecules secreted by microorganisms for Fe ion uptake. The siderophores secreted by microorganisms form stable complexes with Fe(III) ion in nature. The resultant Fe(III)-siderophore complexes are recognized and permeates into microorganisms via their receptor proteins on the cell surface. Artificial siderophores are model compounds mimicking function of natural siderophores. Our group have studied immobilization of microorganisms using Fe(III)-artificial siderophore complexes-modified substrate for the last decade. In this study, we have modified four different Fe(III) artificial siderophore complexes onto Au substrates and have achieved the selective and sensitive microorganism sensing. We have prepared catecholate-type, hydroxamate-type, and their hybrid type artificial siderophores and their Fe(III) complexes. The obtained Fe(III) complexes have been modified onto Au substrates deposited on natural mica. The selective immobilization of several microorganisms onto the substrates have been observed by SEM. The quantitative sensing of microorganisms has been carried out by AC-impedance measurements. In the case of Escherichia coli, the Fe(III)-artificial siderophore-modified substrate have indicated the quantitativeness from 102 to 107 CFU mL–1 and the detection limit of 102 CFU mL–1.

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27.

ラインイメージングセンサを用いたコロニーフィンガープリンティングによる
細菌の菌種判別のロバストネスの向上

東京農工大院工a、株式会社マルコムb

○前田義昌a、田中雄介a、纐纈宏也a、 林 泰圭b
原田 学b、吉野知子a、松永 是a、田中 剛a

IMPROVEMENT OF ROBUSTNESS OF BACTERIAL DISCRIMINATION BASED ON
COLONY FINGERPRINTING USING A LINE IMAGING SENSOR

Yoshiaki MAEDA a, Yusuke TANAKA a, Hiroya KOHKETSU a, Tae-kyu LIM b,
Manabu HARADA b, Tomoko YOSHINO a, Tadashi MATSUNAGA a and Tsuyoshi TANAKA a

a Division of Biotechnology and Life Science, Institute of Engineering,
Tokyo University of Agriculture and Technology,
2-24-16, Naka-cho, Koganei, Tokyo 184-8588
b Malcom Co., Ltd., 4-15-10, Honmachi,
Shibuya-ku, Tokyo 151-0071

Discrimination of the microorganisms contaminating foods, beverages and pharmaceuticals is an important technology for safe manufacturing of these products. Biochemical properties and genetics information of microorganisms have long been utilized for microbial discrimination. However, such analyses require long assay time and skillful operators. To address this issue, we developed a rapid and simple method for discrimination of microorganisms called “colony fingerprinting”. In colony fingerprinting, discrimination was conducted based on analyses of the colony images called “colony fingerprints (CFPs)”, which were obtained using imaging sensors. Currently, a scan system using a line imaging sensor is developed for colony fingerprinting, which allows us to visualize a number of culture plates in one shot, leading to high throughput analysis. Nonetheless, sizes and morphologies of CFPs obtained by the current system were highly divergent in spite of the identical bacterial origins. This might be caused by several possible reasons including high cell density on the medium agar plates as well as inexactness of pre-culture conditions. In this study, we examined these conditions toward precise control of colony formation process and improvement of discrimination robustness of colony fingerprinting.

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28.

農薬グリホサートに対するデュアルリードアウトセンサの創製

東大生研

浅野康一郎、 佐々木由比、 南木 創、 ○南 豪

DUAL READOUT SENSOR FOR HERBICIDE GLYPHOSATE

Koichiro ASANO, Yui SASAKI, Tsukuru MINAMIKI and Tsuyoshi MINAMI

Institute of Industrial Science, The University of Tokyo,
4-6-1 Komaba, Meguro-ku, Tokyo 153-8505

Establishing simple and multimode detection methods for a target is significantly important from the viewpoint of expanding the application potential of chemical sensors. In addition, comparison among the detection methods gives useful insights for constructing rapid, simple, and highly sensitive sensors. In this study, we established a dual method for herbicide glyphosate (GlyP) which is concerned as potent carcinogenic to human. Two types of polythiophene derivatives attached with carboxy groups were employed as fluorophores and as organic semiconductors. Competitive coordination binding among polythiophene derivatives, Cu2+, and GlyP was utilized for the detection of GlyP by a fluorescence chemosensor array and by an organic field-effect transistor (OFET). Both of the methods offered successful detection of GlyP with high sensitivity and selectivity. Thus, our study will open up a new avenue for accurate and multimode detection for one analyte in the field of environmental and clinical chemistry.

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29.

肝機能バイオマーカー測定用電気化学発光センサ

阪大院工a、産総研・先端フォトバイオb

○牧 俊之介a、荒木 晃子b、斎藤 真人a b、 民谷 栄一a b

ELECTROCHEMILUMINESCENT SENSOR FOR LIVER BIO MARKER

Shunnousuke MAKI a, Akiko ARAKI a, Masato SAITO a b, Eiichi TAMIYA a b

a Department of Applied Physics,Graduate School of Engineering, Osaka University,
Suita,Osaka 565-0871
b AIST-Osaka University Advanced Photonics and Biosensing Open Innovation
Laboratory,Photonics Center,
Suita, Osaka 565-0871

Aspartate aminotransferase (AST) is an enzyme that be found abundant in the liver. In the field of disease diagnosis, Serum AST is widely used as a liver function marker. Most of the conventional methods for measuring serum AST require large-scale and invasive experimental systems. This enzyme, however, can also be found in the blood and saliva, in which saliva can be collected non-invasively. If AST in the saliva detection system is established using electrochemiluminescence (ECL) measurement method, it is possible to develop a brand-new diagnosis detection tool. ECL measurement is a promising tool for detection because of its high-sensitivity, rapidity and ability to be incorporated in miniature system. Thus, in this study, the results of the development of a highly sensitive and rapid biomarker measurement sensor system aimed at point-of-care-testing (POCT) by measuring the enzyme activity of AST are reported.

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30.

核酸試料並列分析のための ハイブリッド形成誘引-
電気化学信号変調 センサアレイ

産総研環境管理

○青木 寛、 鳥村 政基、 中里 哲也

SENSOR ARRAYS FOR PARALLEL ANALYSIS OF
OLIGONUCLEOTIDE SAMPLES BASED ON HYBRIDIZATION-AMENABLE
ELECTROCHEMICAL SIGNAL TRANSDUCTION

Hiroshi AOKI, Masaki TORIMURA, and Tetsuya NAKAZATO

National Institute of Advanced Industrial Science and Technology (AIST)
Onogawa 16-1, Tsukuba, Ibaraki 305-8569, Japan

We investigated the feasibility of simultaneous detection of multiple environmentally- and biomedically-relevant RNA biomarker target sequences on a single newly fabricated 384-ch sensor array chip aiming at practical application. The individual sensor is composed of a photolithographically-fabricated Au/Cr-based electrode modified with peptide nucleic acid (PNA) probes. The sensor array chips showed sequence-specific responses upon hybridization of the probes with target sequences complementary to the probes in contrast to mismatch versions. The target oligonucleotides have 15–22 mer sequences from messenger RNAs for estrogen-responsive genes and microRNAs for lung cancer biomarkers. The dependence on target concentrations of sensor responses was observed by using a single chip on which experiments for detection of several target concentrations proceeded simultaneously, with the detection limit of 7.33×10−8 M. As more realistic samples, oligonucleotide samples amplified by PCR from a synthesized template sequence were applied to the chip. They showed sequence-specific responses, revealing the potential for fabricated sensor array chips to be utilized to analyze PCR samples. Unlike complicated and expensive chips that require nanofabrication, our sensor array chips based on glass coated with gold thin films are simple and can be fabricated from inexpensive and readily available materials.

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