Vol. 34, Supplement B (2018)

Proceedings of the 64th Chemical Sensor Symposium
Kanazawa, September 25-26, 2018

 

Abstracts



1.

ストレス計測評価用バイオセンシングデバイスの研究開発
(VIII)唾液硝酸イオン計測によるストレス被験者実験の検討

産総研・阪大先端フォトバイオラボa、神大院人間発達b、阪大院工c
鳥羽商船高専d、東京海洋大学海事e、阪工大工f

○脇田 慎一a,b,c、北村 健一a,d、村井 康二e、大﨑 脩仁a,b、金時 卓哉a,f、森内 隆代f

R & D OF BIOSENSING DEVICES FOR STRESS MONITORING(VIII)
STUDY ON EVALUATION FOR STRESS USING SALIVARY NITRATE MONITORING

Shin-ichi WAKIDAa,b,c、Kenichi KITAMURAa,d、Koji MURAIe、Shuto OSAKIa,b
Takuya KINTOKIa,f and Takayo MORIUCHIf

a AIST PhotoBIO-OIL, National Institute of Advanced Industrial Science and Technology
(AIST), 2-1 Yamada-Oka, Suita, Osaka 565-0871
b Graduate School of Human Development and Environment, Kobe University
3-11 Tsurukabuto, Nada-ku, Kobe, Hyogo 657-8501
c Graduate School of Engineer, Osaka University, 2-1 Yamada-Oka, Suita, Osaka 565-0871
d National Institute of Technology, Toba College, 1-1 Ikegami, Toba, Mie 517-8501
e Faculty of Marine Technology, Tokyo University of Marine Science and Technology
2-1-6 Ecchujima, Koto-ku, Tokyo, 135-8533
f Faculty of Engineering, Osaka Institute of Technology,
5-16-1 Omiya, Asahi-ku, Osaka 535-8585

To develop on new stress-sensing devices for stress science study, we have investigated ISFET (ion-selective field-effect transistor) based biosensors and also have studied organic semiconductor based ISFETs, OFET based biosensors as well as microfluidic TAS (micro total analysis system) for measurement of several salivary stress-marker candidates. During the fundamental studies on the prototype of stress-sensing devices, such as electrophoretic microfluidics and ISFETs for real salivary samples of healthy volunteers, we found salivary nitrate will be a biomarker candidate on the autonomic nervous system response to stress and have already reported the mechanism of salivary nitrate as stress biomarker candidate. To make a scientific study on the stress response mechanism of salivary nitrate, we investigated simultaneous monitoring for healthy volunteers of a ship handling using wearable heart rate monitor and salivary nitrate sampling measurement by prototype of salivary nitrate checkers based on NO3--ISFETs using direct potentiometry. We will introduce the some important results of acute stress subject-experiments for healthy volunteers from the point of view from the autonomic nervous system stress- response of salivary nitrate for mental workload of events using ship handling simulator at Kobe University.

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2.

非酵素的なグルコースなどの電気化学的検出

東北大院薬a、奥羽大薬b

○佐藤勝彦a、小野哲也b、柏木良友b

ELECTROCHEMICAL DETECTION OF GLUCOSE BY NON-ENZYMATIC REACTION

Katsuhiko SATO a, Tetsuya ONO b and Yoshitomo KASHIWAGIb

a Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki,
Aoba-ku, Sendai 980-8578
b School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi,
Koriyama, Fukushima 963-8611

While it is possible to construct a high-performance electrochemical sensor by using an enzyme, such a method may pose a problem with cost, stability or equivalence. Use of an organic electron transfer catalyst such as 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO) instead of an enzyme can eliminate these problems. However, TEMPO showed no significant increase oxidation peaks in the presence of glucose, which indicates that TEMPO not able to catalyze the oxidation of glucose under neutral aqueous solution conditions at 25 °C. We evaluated the applicability of Nortropine N-oxyl (NNO) as nitroxyl radical catalyst and examined the electrochemical detection of glucose. NNO exhibited high electrocatalytic activity for the oxidation of glucose in a near neutral pH phosphate buffer solution at 25 °C. This electrocatalytic method was shown to be useful for the oxidation of choline and lactate, and would thus be applicable to the quantitative analysis of a variety of carbohydrates and alcohols.

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3.

メソポーラス白金電極を用いた電気化学
グルコースセンシングにおける妨害物質の影響

中央大学

○篠原 そのこ、 松永 真理子

THE EFFECT OF INTERFERING SPECIES ON ELECTROCHEMICAL GLUCOSE
SENSING USING THE MESOPOROUS PLATINUM ELECTRODE

Sonoko SHINOHARA and Mariko MATSUNAGA

Chuo University,
1-37-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551

To avoid the effect of interfering species is one of the most important subjects in non-enzymatic glucose sensing. In our previous study, a mesoporous platinum, which has a lot of high index surface, shows high sensitivity for electrochemical glucose sensing. In this paper, the effects of L-ascorbic acid (AA) and 4-acetamidophenol (AP), which are interfering species often included in the actual analyte, was investigated using cyclic voltammetry (CV). As a result, the oxidation peaks of glucose and AA are appeared to be overlapped. In addition, the peak current density of D-glucose increased with increasing electrolyte concentration, while that of AA did not change. Moreover, the oxidation peak of AA shifts with increasing electrolyte concentration. Therefore, selectivity is demonstrated to be improved by adjusting the electrolyte concentration.

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4.

分子インプリント高分子固定カーボンペースト電極を
用いた血中バンコマイシンセンサの開発

芝浦工大応用化学科a,芝浦工大共通学群化学科目b

○林 俊介a、 吉見 靖男a、 幡野 明彦b

DEVELOPMENT OF BLOOD VANCOMYCIN SENSOR USING
MOLECULARLY IMPRINTED POLYMER GRAFTED CARBON PASTE ELECTRODE

Shunsuke HAYASHI a , Yasuo YOSHIMI a , Akihiko HATANO b

a Department of Applied Chemistry, Shibaura Institute of Technology,
3-7-5, Toyosu, Koto-ku, Tokyo 135-8548
b Department of Chemistry, Shibaura Institute of Technology,
307 Fukasaku, Minuma-ku, Saitama City, Saitama 337-8570

Antimicrobial resistance (AMR) is a global problem in medicine. Therapeutic drug monitoring (TDM) of antibacterial drugs are necessary for overcoming the AMR problem. Vancomycin (VCM) is a typical antibacterial agent which requires a strict TDM. In this study, we developed a VCM senor with a paste electrode of graphite grafted with molecularly imprinted polymer (MIP). Cyclic voltammetry performed using the MIP-electrode in whole blood or PBS containing ferrocyanide as a redox marker and VCM as an analyte. And the sensitivity of this electrode was slightly higher for PBS than for blood. However, blood sensitivity is also sufficiently high. Therefore, the MIP electrode is useful as a VCM sensor. Furthermore, this sensor can be measured easily and in a short time. Therefore, the electrode would be potential tool to suppress the creation of VCM-resistant bacteria.

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5.

分子インプリント高分子固定カーボンペースト
電極を用いたドーパミンセンサ

芝浦工大

○吉見 靖男,日高 愛子

A DOPAMINE SENSOR USING A PASTE ELECTRODE OF GRAPHITE ELECTRODE
GRAFTED WITH MOLECULARLY IMPRINTED POLYMER

Yasuo YOSHIMI, Aiko HIDAKA

Department of Applied Chemistry, Shibaura Institute of Technology,
Toyosu, Koto-ku, Tokyo 135-8548

Dopamine is an important neurotransmitter working at central nerve system of animals. A selective sensor of dopamine is required for basic research of neuroscience and monitoring of therapeutic effect of Parkinson’s disease. The purpose of the research is the development of electrochemical sensor of dopamine using molecularly imprinted polymer (MIP). A poly (sodium styrene 4-sulfate -co- 3-(acrylamido) phenylboronic acid -co-methylenebisacrylamide) imprinted by dopamine was grafted on graphite particles. The graphite was mixed with silicone oil and mortared. The mixture was packed to the tip of the glass capillary to fabricate paste electrodes. Differential pulse voltammetry was performed with the paste electrode in the solution of dopamine or adrenaline. The anodic current of dopamine at the MIP-grafted paste electrode was higher than the adrenaline although the electroactivity at the ungrafted graphite paste electrode of adrenaline was higher than that of dopamine. The results indicate that the specific interaction between the dopamine and the imprinted cavity in the MIP enhance the electroactivity of dopamine. The effect would enable an electrochemical sensor which can detect dopamine in central nervous system directly and selectively.

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6.

プルシアンブルー類似体のレドックス機能を
利用した全固体型マグネシウムおよび
カルシウムイオン選択性電極

東京理科大学a、KOA株式会社b

○土屋 和彦a、堀場 達雄a、武居 祐子b、高山 利治b、駒場 慎一a

ALL-SOLID-STATE MAGNESIUM AND CALCIUM ION SELECTIVE ELECTRODES
BY USING A REDOX FUNCTION OF PRUSSIAN BLUE ANALOGUES

Kazuhiko TSUCHIYA a, Tatsuo HORIBA a, Yuko TAKEIb, Toshiharu TAKAYAMA b
and Shinichi Komaba a

aDepartment of Applied Chemistry, Tokyo University of Science
1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601
b KOA Corporation
14016, Oaza Naka-minowa, Minowa-machi, Kamiina-gun, Nagano 399-4697

We have developed all-solid-state Mg and Ca ion-selective electrodes by using a cation insertion material placed between metal substrate and ion selective membrane. Prussian blue analogues were employed as insertion materials which allow Mg2+ or Ca2+ ions to be reversibly inserted/extracted into/from their crystal structures associated with electrochemical redox reactions. The double-layered electrodes formed on Pt, Pt/cation insertion material/ISM, exhibited an ideal Nernstian response in the range of 10-5 – 10-1 mol dm-3 with high selectivity for targeted ion and good potential stability. AC impedance analysis of the all-solid-state electrodes revealed that the insertion materials were capable of reducing the electrode impedance at the interface between the metal substrate and ion selective membrane, which was responsible for their improved potential stability.

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7.

IS-FETを利用したオイル劣化評価用センサの開発

長崎大院工

○靏岡未央子、上田太郎、鎌田 海、兵頭健生、清水康博

DEVELOPMENT OF IS-FET-BASED SENSORS FOR DIAGNOSIS OF OIL
DETERIORATION

Mioko TSURUOKA, Taro UEDA, Kai KAMADA, Takeo HYODO and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

The deterioration of machine lubricants and engine oils can be commonly estimated through total acid number (TAN), which is the acidity measured by a general titration using KOH(aq.), but the technique is very complicated and the online monitoring of TAN is quite difficult at present. Therefore, monitoring of acidity of a general mineral oil has been attempted by utilizing ion-sensitive field-effect transistors (IS-FETs), which are able to detect accurate pH in aqueous solution quickly. Coating of the surface of IS-FETs with a cation exchange membrane (CEM) was effective in improving the acidity-monitoring properties. In addition, the optimization of the CEM thickness and the addition of selected ion liquids into CEM further improved the magnitude of response and the response speed, probably because of an increase in cation conductivity of CEM.

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8.

コンゴーレッド固定化FETバイオセンサを用いた
ヒト血清中アミロイドβ凝集体の検出

早大ナノ・ライフ機構a、早大院先進理工b

◯黒岩 繁樹a、 林 宏樹b、 秀島 翔a、 門間 聰之a,b、 逢坂 哲彌a,b

DETECTION OF AMYLOID β FIBRILS IN HUMAN SERUM
USING CONGO-RED IMMOBILIZED FET BIOSENSOR

Shigeki KUROIWA a, Hiroki HAYASHI b, Sho HIDESHIMA a,
Toshiyuki MOMMA a,b and Tetsuya OSAKA a,b

a Research Organization for Nano & Life Innovation, Waseda University,
Shinjuku, Tokyo 162-0041
bGraduate School of Advanced Science and Engineering, Waseda University,
Shinjuku, Tokyo 169-8555

A field effect transistor (FET) biosensor is a promising electrochemical device for detection of various biomarkers due to label-free and rapid sensing technique. Recently, the number of patients with Alzheimer’s disease (AD) increases especially in advanced country. Aβ(1-42), one of an amyloid-β protein (Aβ), causes neuronal cell death due to the aggregation and deposition in brain. Therefore, detection of aggregates (fibrils) of Aβ(1-42) is important in the initial stage. Previously, we have developed a simple detection method of Aβ(1-42) fibrils using Congo-red (CR)-immobilized FET biosensors for early-stage diagnosis of AD. In order to use this biosensor at hospital or home, the biosensor need to detect Aβ(1-42) fibrils in minimally invasive biological samples such as human serum (HS). However, human serum albumin (HSA), the most abundant protein in HS, forms a complex with Aβ. In this study, to investigate the interference of HSA on the detection of Aβ(1-42) fibrils in HS samples, we measured FET responses related to reaction time of Aβ(1-42)-HSA complex and CR-immobilized surface.

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9.

紙・フィルム・テープでつくる低コストな
診断用チップ/検査キット

産総研健康工学

○渕脇 雄介、 田中 正人、 藤原 貴久、 兼田 麦穂、 山村 昌平

SIMPLE AND HIGH-PERFORMANCE DIAGNOSTICS TEST CHIP & KIT USING PAPER,
FILM AND TAPE

Yusuke FUCHIWAKI, Masato TANAKA, Takahisa FUJIWARA,
Mugiho KANEDA, Shohei YAMAMURA
Health Research Institute, National Institute of Advanced Industrial Science and
Technology, Takamatsu-shi, Kagawa 761-0301

Although paper-based microfluidic chip devices are an ideal platform for point-of-care diagnostics, it is difficult to achieve microfluidic control required for sensitive analyses such as ELISA on a paper substrate. In this study, we developed a novel lateral-flow test chip that can perform operations like a pump, such as flowing, stopping, and replacing a solution, just by adding the solution onto an inlet. For sensitive and accurate detection, the transparency and flatness of the substrate is crucial for precise analysis of weak light generated by a specific reaction. The signal intensity of the novel chip was higher than that of a chip made of nitrocellulose membrane, and the variation was smaller. The limit of detection of the chip was 0.1 ng•ml-1, whereas that of the nitrocellulose membrane was 100 ng•ml-1.

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10.

金属で接続された分離型微小3電極系の挙動

筑波大院数理物質

○假屋 洸希、 佐藤 優成、 栗原 寿明、 鈴木 博章

BEHAVIOR OF A MICROFABRICATED THREE-ELECTRODE SYSTEM WITH 
SEPARATED ELECTRODE COMPARTMENTS CONNECTED WITH METALJUNCTIONS

Koki KARIYA, Yusei SATO, Toshiyuki KURIHARA and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, University of Tsukuba,
1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573

A miniaturized three-electrode system was fabricated and the behavior was examined. The working, reference, and auxiliary electrodes were accommodated in separate compartments. Solutions in the compartments were connected either with liquid junctions or metal junctions. When the working electrode compartment was connected to the reference and auxiliary compartments separately with Ag/AgCl or platinum wires, cyclic voltammograms obtained using the device were close to those obtained using the same device connected with liquid junctions. On the other hand, when the compartments were connected in the order of the working-reference-auxiliary electrode compartments, the redox peaks were more separated and the peak currents decreased. The obtained results indicate that the metal junctions work as an alternative to the liquid junctions.

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特別講演

多機能バイオプローブとしての
磁性-プラズモンハイブリッドナノ粒子

北陸先端大マテリアル

○前之園 信也

MAGNETIC-PLASMONIC HYBRID NANOPARTICLES
AS MULTIFUNCTIONAL BIOPROBES

Shinya MAENOSONO

School of Materials Science, JAIST, 1-1 Asahidai, Nomi, Ishikawa 923-1292

Magnetic-plasmonic hybrid nanoparticles in which ferromagnetic and plasmonic components were mixed at the atomic level have gained immense attraction as the capable probes for various bioapplications such as bioimaging, biosensing, immunodiagnosis, and hyperthermia. There is a dramatic expansion in development of novel hybrid nanoparticles with improved properties in the last two decades. In this talk, synthetic protocol, structure and physical properties of magnetic-plasmonic hybrid nanoparticles will be discussed. Finally, imaging and magnetic separation of cellular organelles such as autophagosomes using the hybrid nanoparticles will be presented. The presented content will be helpful for the people who considers to fabricate nanoparticles for bioapplications.

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清山賞受賞講演1

医療・バイオ分野での利用に資する
アンペロメトリックセンシングチップの開発

東北大院環境

○井上(安田)久美

DEVELOPMENT OF AMPEROMETRIC SENSOR CHIPS
FOR MEDICAL AND BIOLOGICAL FIELDS

Kumi Y. INOUE

Graduate School of Environmental Studies, Tohoku University,
6-6-11 Aoba, Aramaki, Aoba, Sendai 980-8579

This paper reviews our development of chip-type amperometric sensor for medical and biological sensing use, including amperometric protease assay method and its appli-cation to endotoxin sensor and cell apoptosis sensor, whole cell biosensors, mul-ti-electrochemical sensing platform “Bio-LSI”, and amperometric sensor with bipolar elec-trochemistry. These novel and unique sensing methods and devices will be used for wide fields such as medical diagnosis, home healthcare, environmental monitoring and biological research as portable, simple and convenient measurement tools.

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11.

吸水性ポリマーを用いた
マイクロ流路内の流体駆動システムの開発

神奈川産技総研1、東大生研2

○山田 哲也1、神谷 厚輝1、大崎 寿久1、 竹内 昌治1, 2

FLUSHING DEVICE FOR LATERAL FLOW SENSOR
USING SUPER ABSORBENT POLYMER

Tetsuya Yamada1, Koki Kamiya1, Toshihisa Osaki1, and Shoji Takeuchi1, 2

Kanagawa Institute of Industrial Science and Technology (KISTEC) 1
Institute of Industrial Science (IIS), The University of Tokyo 2

This work presents a pumpless flushing system driven by super absorbent polymer for repeatable detection. The repeatability is an important point to sensor. In addition to repeatability, a portable device working with no battery is desirable. To realize a portable sensor that can repeatedly detect sample, a flushing system working without electric supply is required. To develop this kind of sensor, we focus on superabsorbent polymer and MEMS (Micro Electro Mechanical Systems). We propose a continuous flushing system driven by super absorbent polymer and demonstrated the validity of our system by repeated measurement of the hypochlorite as analyte. Electromotive force generated between detection well and reservoir well was utilized to detect hypochlorite. In this sensor, ion exchange membrane was not required because of continuous microfluidic flow. We believe that this compact, automatic, pumpless flushing system working with no complex instruments enables fast and repeatable samples detection.

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12.

エピゲノムのオンチップ分析向けた
アルキル化リンカーの創成

国立研究開発法人産業技術総合研究所

○栗田僚二、栗之丸 隆章、小島 直

ALKYLATION LINKER FOR ON-CHIP EPIGENOME ANALYSIS

Ryoji KURITA, Takaaki KURINOMARU and Naoshi KOJIMA

Biomedical Institute, National Institute of Advanced Industrial Science and Technology
(AIST),
1-1-1 Higashi Tsukuba, Ibaraki 305-8566

The immobilization of DNA on a solid support is a versatile process with wide-ranging applications to, for example, a DNA-based biosensor and a microarray technique. In this study, a bifunctional linker molecule containing nitrogen mustard and a cyclic disulfide group (L1) has been developed for the covalent immobiliza-tion of DNA duplex in a neutral solution with no need for hybridization, adapter ligation or terminus modification. The nitrogen mustard of L1 is bound to interstrand guanine bases and crosslinked with DNA strands. Surface plasmon resonance (SPR) revealed that DNA cross-linked with L1 was successfully immobilized on a gold surface owing to the bivalent thiolate-gold linkage. A quantitative analysis of epigenomic modification in immobilized DNA was achieved with SPR-based immune sensing. A one-step covalent immobilization approach under biological conditions using L1 will be an effective way of performing a genome-wide analysis of extracted DNA without further pretreatment.

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13.

トライモード光導波路干渉計型
マイクロ化学センサ

筑波大院数理物質

海老原 恵都、 内山田 健、 浅川 潔、 鈴木 博章

MICROFABRICATED CHEMICAL SENSOR BASED ON
A TRIMODAL OPTICAL WAVEGUIDE INTERFEROMETER

Keito EBIHARA, Ken UCHIYAMADA, Kiyoshi ASAKAWA, and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, Tsukuba University,
1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573

Sensing devices based on a trimodal optical waveguide interferometer was developed. The interferometer used the interference between the fundamental mode and the second order mode to improve detection sensitivity in contrast to a previously reported bimodal waveguide interferometer that uses the interference between the fundamental mode and the first order mode. The devices were designed based on simulation results. A single and double input/output waveguide structures were tested. The devices were actually fabricated by electron beam lithography using a negative photoresist (SU-8) as the waveguide material. The results obtained by experimental characterization agreed well with those by simulation. Finally, applicability of the device to glucose sensing was demonstrated.

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14.

細胞捕捉用マイクロ流路を用いたサルモネラ菌の
検出

創価大

久保いづみa,橋本弘実a

DETECTION OF SALMONELLA ENTERICA UTILIZING A MICROFLUIDIC DISC FOR
CELL ENTRAPMENT

Izumi KUBOa, Hiromi HASHIMOTOa
aGraduate School of Engineering, Soka University
Hachioji, Tokyo, 192-8577

Conventionally, food borne bacteria have been examined by cultural method, and it takes several days to get the result. So, simple and rapid method is required. Immunostaining of bacteria can shorten detection time and enable specific detection of bacteria. Among food borne bacteria, we focused on Salmonella enterica. Microfluidic disc for immunostaining was designed and fabricated from PDMS in our laboratory. The shape of trap on a microchannel is like cup. In order to reduce the amount of antibody reagent, microfluidic disc device with cup like trap was utilized for the detection of S. enterica cells in this study. We tried to perform immunostaining of the cells on the disc. HRP labeled polyclonal goat anti salmonella antibody was used to the immunostaining of S. enterica cells. First, microchannels were blocked by BSA solution. Then, S. enterica cell suspension, antibody, and substrate of HRP ware injected into the inlet of the microchannel, and flow through it by rotation of the disc respectively. S. enterica and antibody reacted for 15 minutes. We observed the reaction under fluorescent microscope and measured fluorescent intensity every 10 seconds for 1 minute. Twenty seconds after irradiation of excitation light, we can distinguish between with and without S. enterica cells. In this study, we can detect S. enterica cells only by simple manipulation, such as rotation of the disc.

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15.

神経伝達物質イメージング用の
蛍光性分子インプリントナノ粒子の開発

芝浦工業大学a

○勝俣 湧斗a、森 莉紗子a、 吉見 靖男a

DEVELOPMENT OF FLUORESCENT MOLECULARLY IMPRINTED NANOPARTICLES
FOR NEUROTRANSMITTER IMAGING

Yuto KATSUMATAa , Risako MORIa , and Yasuo YOSHIMIa

a Department of Applied Chemistry, Shibaura Institute of Technology,3-7-5,Toyosu, koto-ku
Tokyo, 135-8548

Analysis of the action of neurotransmitters in nervous system is important for elucidating the mechanism of neural network in nervous system. Then development of a probe which can track a specified neurotransmitter in real time with high selectivity has been required. Molecularly imprinted polymer (MIP), which is a molecular recognition material obtained by polymerization with a template-effect of the target molecule, may be feasible as the probe. In this study, nanoparticle of MIP including fluorescent group (fMIP-NP) were developed as the probe of neurotransmitters. The fluorescent intensity and the radius of the fMIP-NP was increased by addition of template but was insensitive to template analog. After adsorbing fMIP-NP to a cerebral ganglion were observed by the fluorescent imaging system. As a result, only one neuron near the serotonergic neurons (MCC) indicated spiked change of 0.2~0.3 % in the fluorescent intensity an interval of 50 ms.

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16.

2次元リアルタイム蛍光測定システムと
それを用いた簡易DNAセンサの開発

横河電機株式会社 マーケティング本部a,東京農工大学 大学院 工学研究院b

○田口 朋之a,田名網 健雄a,吉野 知子b,松永 是b,田中 剛b

DEVELOPMENT OF PRACTICAL GENE ANALYTIC SENSORS BY USING
REAL-TIME FLUORESCENT MEASUREMENT SYSTEM

Tomoyuki TAGUCHI a, Takeo TANAAMI a, Tomoko YOSHINO b, Tadashi MATSUNAGA b,
and Tsuyoshi TANAKA b

a Yokogawa Electric Co., 2-9-32 Nakacho, Musashino, Tokyo 180-8750
b Institute of Engineering, Tokyo University of Agriculture and Technology,
2-24-16, Naka-cho, Koganei, Tokyo 184-8588

Fluorescence measurement of biomolecules is the powerful tool for getting information of many kinds of biological samples and is utilized as the universal tool in this field. We have developed two-dimensional imaging system consisted of a laser light source, a microlens array for generation of a multi-beam laser, and a CCD for real-time detection. We applied this system for the absolute fluorescence intensity measurement. This technology is definitely a step forward for fluorescence measurement. Our measurement system is consisted of elaborate optics and calibration system. Since it has the traceability to the national standard and gives measured value as International Unit, repeatability and compatibility are quantitatively guaranteed. Furthermore, we have developed signaling array technology for detecting nucleic acids with a sensing probe, which hybridizes with specific nucleic acids and emits fluorescence. This probe enables conventional DNA microarrays used for microbial tests to offer one-step detection of unlabeled nucleic acid fragments, thus simplifying the detection of microbes. This paper describes details of this signaling array technology and its applications.

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17.

コロニーフィンガープリントの機械学習基づく
真菌Aspergillus属の菌種判別法の開発

東京農工大院工a、株式会社マルコムb 

○杉山由依a、土橋弘典a、吉野知子a、前田義昌a、林泰圭b、原田学b、松永 是a、田中 剛a

DEVELOPMENT OF A DISCRIMINATION METHOD FOR ASPERGILLUS SPECIES
BASED ON MACHINE LEARNING OF COLONY FINGERPRINTS

Yui SUGIYAMA a, Yoshiaki MAEDA a, Tae-kyu LIM b, Manabu HARADA b, Tadashi
MATSUNAGA a, Tomoko YOSHINO a and Tsuyoshi TANAKA a

a Institute of Engineering, Tokyo University of Agriculture and Technology,
2-24-16, Naka-cho, Koganei, Tokyo 184-8588
b Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071

Discrimination of fungi Aspergillus species is important in food-contamination testing as well as clinical diagnosis. Conventionally, microscopic observation of cell morphology, genetic analyses and immunoassay are employed for this purpose. However, these methods are time-consuming and labor-intensive. We have proposed a rapid and simple method for discrimination of microorganisms called “colony fingerprinting”. In colony fingerprinting, discrimination was carried out based on multivariate analyses of the colony images, called “colony fingerprints”, which were obtained by a wide-field of view lens-less imaging system. The imaging system is composed of a complementary metal oxide semiconductor (CMOS) sensor and LED light, and enables to visualize microcolonies with the size of sub-millimeter scale. Therefore, the assay time can be short as compared to conventional methods. In our previous study, we demonstrated that colony fingerprinting was useful for discrimination of bacteria. In this study, we applied this method for discrimination of four closely related Aspergillus species (A. oryzae, A. niger, A. nomius, and A. flavus). Using support vector machine (SVM) as a classifier, discrimination of these fungi was successfully demonstrated with 95% of accuracy. This result suggests that the colony fingerprinting is a promising method for fungal discrimination.

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18.

プローブナノ粒子の拡散性に基づいた
新規DNAセンサの開発

福井大院工a、國立成功大學生物醫學工程學系b

○一木 啓志a、童俞 靜b、莊漢 聲b、坂元 博昭a、末 信一朗a

DEVELOPMENT OF NEW DNA SENSOR BASED ON DIFFUSION OF PROBE
NANOPARTICLES IN LIQUID

Keishi ICHIKI a, Yu-Ching TUNG b, Han-Sheng CHUANG b, Hiroaki SAKAMOTO a,
Shin-ichiro SUYE a

a Frontier Fibre Technology and Science, Graduate School of Engineering, University of
Fukui
Fukui-shii, Hukui 910-8507
b Biomedical Engineering, National Cheng Kung University,
No.1, University Road, Tainan City 701, Taiwan (R.O.C)

The rapid and easy detection of biomolecules is a very important technique for early detection of disease and prevention of the spread of harmful microorganisms. Molecular recognition by hybridization of DNA is often used for biomolecule detection. The method has high selectivity and is also versatile. So far, we have developed a sensing system that captures targets DNA and electrochemically detects them with nanoparticles modified with base chains complementary to target DNA1). This system provided selective detection of pico-mol level against targets. However, this system requires apparatus for electrochemical measurement, and B / F separation step is necessary. Therefore, in this research, we focused on a technique that analyzes the diffusion behavior of particles in liquid2). We designed a system to quantify DNA from its diffusion behavior by forming a complex with probe particles (Fig. 1). We expect that when the target DNA forms a complex with nanoparticles, the diffusion rate will decrease due to the increase in particle size. It is considered that the size of DNA complex increases with increasing DNA concentration and diffusibility decreases.

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19.

アプタマー修飾微粒子の誘電泳動挙動と
BF分離不要なセンサへの応用

兵庫県大院物質理学

○安川 智之、岡崎 仁、鈴木 雅登

BIOSENSORS BASED ON DIELECTROPHORETIC PROPERTIES OF PARTICLES
MODIFIED WITH APTAMER

Tomoyuki YASUKAWA, Hitoshi OKAZAKI, Masato SUZUKI

Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako,
Hyogo 678-1297

A simple detection system based on dielectrophoresis (DEP) of particles modified with aptamer has been investigated in the presentation. The aptamer for thrombin (model target in this presentation) was immobilized on the surface of particles (500 nm diameter) via crosslinking agent (sulfo-SMCC). The suspension of the particles modified with aptamer was mixed with solutions containing different concentrations of thrombin, and injected into the channel of devices for the DEP manipulation. The interdigitated array of microband with the shape of the teeth of a saw was arranged in the channel for the particle manipulation based on the DEP. The particles were broadly accumulated above the band electrode by applying AC voltage with high frequency region due to the effect of negative DEP. In contrast, the particles were trapped at the region between the teeth of a saw with low frequency region by the effect of positive DEP. This, we can easily find the cross-over frequency which is the frequency without forces of both positive and negative DEP. The cross-over frequencies for the particles in different concentrations of thrombin were estimated. The cross-over frequency decreased with increasing the concentration of thrombin. The decrease of the cross-over frequency could be due to the decrease of the surface conductivity of particles by capturing of thrombin to the immobilized aptamer. The present procedure is absolutely simple because of the needless of the separation step for unreacted thrombin.

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20.

異種元素を添加したマグネシウムフェライトの
CO ガス検知特性

北九州高専

○小畑 賢次、松嶋 茂憲

EFFECT OF ANOTHER ELEMENT ON CO SENSING PROPERTIES OF MgFe2O4

Kenji OBATA, Shigenori MATSUSHIMA

Department of Creative Engineering, National Institute of Technology (NIT),
Kitakyushu College, Shii 5-20-1, Kokuraminami-ku, Kitakyushu 802-0985

M-added (M = Al, La) and pure MgFe2O4 powders were prepared from a malic acid complex, and the CO sensing properties were examined in the temperature range of 250 to 500 °C in dry air. In the XRD measurement, when M atoms were added to MgFe2O4, the crystallite growth of MgFe2O4 was suppressed remarkably in comparison with the pure material. The CO sensitivity of pure MgFe2O4 was confirmed only at 500 ºC. On the other hand, for 5 mol% La-added MgFe2O4, the CO sensitivity was improved in comparison with pure material and was estimated to be 2.9 times higher than that of pure one at 400 °C. Furthermore, 10 mol% La-added MgFe2O4 showed a higher CO sensitivity than that of 5 mol% La-added MgFe2O4 at 350 ºC.

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21.

MoS2ナノシートの新規合成方法の開発
とガス検知特性評価

熊大院自然a、熊大院先端b、九大院総理工c、熊大グローバル教育d

○前田 佳也a、 中島 かれんa、 船津 麻美b、 末松 昂一c、Armando T. Quitain d
佐々木 満a、木田 徹也a

DEVELOPMENT OF NEW SYNTHESIS METHOD OF MoS2 NANOSHEETS AND THEIR
GAS SENSING PROPERTIES

Kana MAEDA a, Karen NAKASHIMA a, Asami FUNATSU b, Koichi SUEMATSU c,
Armand T. Quitain d, Mitsuru SASAKI a, and Tetsuya KIDA a

a Faculty of Advanced Science and Technology、 Kumamoto University、
Kumamoto 860-8555、
b Faculty of Advanced Science and Technology、 Kumamoto University、 c Interdisciplinary Graduate School of Engineering Sciences、 Kyushu University、
Kasuga-shi、 Fukuoka 816-8580、
d Global College、 Kumamoto University

Determination of ethanol concentration in breath air is important to avoid drunk-driving incidents. Hence, compact gas sensors are required to detect ethanol concentration accurately, quickly, and highly sensitively. For such applications, semiconductor gas sensors are promising; they can detect combustible gases at low concentrations. In this study, we focused on layered transition metal dichalcogenides (LTMDs) as new sensor materials, which are two-dimensional materials with semiconductor properties. The two-dimensional nature of these materials allows for the miniaturization the device and highly sensitive detection of gases. Indeed, there has been literature reporting that molybdenum disulfide (MoS2) binds strongly to volatile organic compounds. Here, we show that MoS2 nanosheets synthesized by a new liquid phase method exhibited a good sensing response to ethanol.

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22.

酸化セリウム酸素センサが受ける干渉ガスの影響

産総研a、大陽日酸b

〇伊藤敏雄a、伊豆典哉a、赤松貴文a、申ウソクa、三木雄輔b、廣瀬泰夫b

EFFECT OF FLAMMABLE INTERFERENCE GASES FOR CERIUM OXIDE OXYGEN
SENSOR

Toshio ITOH a, Noriya IZU a, Takafumi AKAMATSU a, Woosuck SHIN a,
Yusuke MIKI b, Yasuo HIROSE b

a National Institute of Advanced Industrial Science and Technology (AIST),
Shimo-shidami, Moriyama-ku, Nagoya 463-8560
b Taiyo Nippon Sanso Corporation, 10 Okubo, Tsukuba, Ibaraki 300-2611

Cerium oxide possesses high diffusion coefficient for oxygen. Oxygen vacancies in surface of cerium oxide, which depend on oxygen pressure, can diffuse into its bulk. The electric resistance of cerium oxide is affected by its oxygen vacancies. The cerium oxide thick films can be used as resistive oxygen sensors. Ten percent of zirconium-doped cerium oxide (CeZr10) possesses the same oxygen sensing property as the cerium oxide and resistance level of the CeZr10 is around one-tenth of that of cerium oxide. However, the oxygen vacancies of cerium oxide and CeZr10 sensors are also developed by oxidation of combustible gases, so the cerium oxide and CeZr10 sensors cannot monitor to oxygen concentration in atmosphere with the same or more concentrations of combustible gases. In this study, we have developed catalytic layers on the CeZr10 sensor thick films for consumption of interference combustible gases.

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23.

半導体ガスセンサの可燃性ガス検知特性に及ぼす
ヘキサメチルジシロキサンの影響

長崎大院工a、ホーチキb

○兵頭健生a、福浦弘人a、岡安克也b、万本 敦b
鎌田 海a、上田太郎a、清水康博a

EFFECTS OF HEXAMETHYLDISILOXANE ON SENSING PROPERTIES OF
SEMICONDUCTOR GAS SENSORS TO INFLAMMABLE GASES

Takeo HYODOa, Hiroto FUKURAa, Katsuya OKAYASUb, Atsushi MAMMOTOb,
Kai KAMADAa, Taro UEDAa, and Yasuhiro SHIMIZUa

aGraduate School of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
bResearch and Development Center, Hochiki Corporation,
3-7-1 Minamimachida, Machida, Tokyo 194-8577, Japan

Effects of surface modification of a SnO2 sensor with hexamethyldisiloxane (HMDSO) at 400°C on the sensing properties to hydrogen (H2) and methane (CH4) have been investigated in this study. The resistance and the magnitude of CH4 response of the SnO2 sensor monotonically decreased with an increase in the amount of HMDSO modification. On the other hand, the small amount of HMDSO modification enhanced the magnitude of H2 response of the SnO2 sensor, probably because H2 molecules easily diffused through a resultant SiO2-based layer into the SnO2 surface in comparison with O2 molecules, while the further increase in the amount of HMDSO modification decreased even the magnitude of the H2 response. In addition, the HMDSO modification decreased both H2 and CH4 sensitivities of the SnO2 sensor, and the decrease in H2 sensitivity by the HMDSO modification was significant, in comparison with that of the CH4 sensitivity.

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24.

熱線型半導体式高感度VOCセンサの開発

新コスモス電機

〇大石 達也、北川 靖久、三橋 弘和、中谷 幹哉

DEVELOPMENT OF HOT WIRE SEMICONDUCTOR TYPE
HIGH SENSITIVE VOC SENSORS

Tatsuya OHISHI, Yasuhisa KITAGAWA, Hirokazu MITSUHASHI and Mikiya NAKATANI

New Cosmos Electric Co.,Ltd.
2-5-4 Mitsuya-naka, Yodogawa-ku, Osaka 532-0036, Japan

Hot wire semiconductor type high sensitive gas sensors for the detection of volatile organic compounds (VOC) have been developed. The VOC sensor consists of platinum wire and sintered SnO2 carrying specific metal oxides. The fabricated sensor showed high sensitive to the VOC, and it showed excellent poisoning durability against siloxane gas. The addition of the metal oxide caused a change in the oxidation activity of SnO2, and the sensor characteristics were improved. VOC sensors fabricated by micro electro mechanical systems (MEMS) technology have been also developed. The power consumption of this sensor is about 300μW. The MEMS type gas sensor showed high sensitive to the VOC.

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25.

A GAS-SENSITIVE SPIM SENSOR WITH
SUSPENDED GATE FOR DETECTION OF ETHANOL
VAPOR USING SnO2 AS SENSING ELEMENT

東北大院工a、東北大院医工b

○王孟云a、チューン ホアンアンb、フレデリック ヴェルナーa、宮本 浩一郎a、吉信 達夫a, b

a Graduate School of Engineering, Tohoku University,
6-6-05 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8579
b Graduate School of Biomedical Engineering, Tohoku University,
6-6-12 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8579

In this study, a gas-sensitive SPIM sensor using SnO2 as sensing element is proposed, to detect local impedance changes on the sensing surface in response to ethanol vapor. This gas-imaging sensor has a field-effect structure with an air gap to form a suspended-gate structure. The local impedance changes due to the interaction between ethanol vapor and SnO2 film are observed by monitoring the photocurrent signal induced by a scanning laser beam. Furthermore, the photocurrent signal is captured in a spatially resolved manner and converted into images thanks to the light-addressability of SPIM, which can be used for visualization of the gas-concentration distribution in the gas channel.

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26.

AlGaN/GaNへテロ構造トランジスタを基盤とする
高感度化学センサの検討

北大量集センター

○小松 祐斗、植村 圭佑、渡久地 政周、佐藤 威友

STUDY ON HIGH SENSITIVE CHEMICAL SENSORS BASED ON
AlGaN/GaN HETEROSTRUCTURE TRANSISTORS

Yuto KOMATSU, Keisuke UEMURA, Masachika TOGUCHI and Taketomo SATO

Research Center for Integrated Quantum Electronics, Hokkaido University,
Sapporo-shi, Hokkaido 060-0813

We investigated the ion-sensitive field-effect-transistors (ISFETs) based on the AlGaN/GaN heterostructures for the chemical-sensor application. In order to improve the transconductance (gm) of open-gate ISFETs, the photo-electrochemical (PEC) etching was applied on AlGaN surface for the formation of the recessed-gate structure. The etching depth of AlGaN layer was precisely controlled by optimized PEC condition, where very smooth etched-surface was obtained with a RMS roughness of 0.6 nm. The Ids-Vds characteristics of AlGaN/GaN ISFETs were measured in a PBS electrolyte by applying the gate voltage using an Ag/AgCl electrode. The current saturation and pinch-off behavior were clearly observed. This indicates the current transport in AlGaN/GaN heterostructures was modified by the surface potential changed at open-gate area. As expectedly, the threshold voltage, Vth, was changed with the AlGaN thickness, where the normally-off operation (Vth > 0V) was obtained in the recessed-gate ISFETs with AlGaN thickness of 7.6 nm. The Ids-Vds curves of the recessed-gate ISFET showed a strong dependence on the pH value of the electrolyte. The pH-sensitivity was roughly estimated about 65µA/pH, corresponding to the gm value of 1.1 mS. The values were 1.3 times larger than those of the planar-gate ISFET.

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27.

NIPAM-BMA共重合体微粒子のHClガス吸着脱離特性

愛媛大院理工

○有川 大生、松口 正信

HCl GAS ADSORPTION/DESORPTION CHARACTERISTICS OF
NIPAM-BMA COPOLYMER NANOPARTICLES

Daiki ARIKAWA, Masanobu MATSUGUCHI

Department of Materials Science and Biotechnology, Graduate school of Science and
Engineering, Ehime University, 3,Bunkyo-cho,Matsuyama 790-8577

Poly(N-isopropylacrylmide–co-Butyl methacrylate)s (P(NIPAM-BMA)) with the composition of NIPAM:BMA=10:1 and 20:1 were synthesized via free radical polymerization. A lower critical solution temperature (LCST) of these copolymers in aqueous solution were 19℃ for NIPAM : BMA = 10:1 and 14℃ for 20:1. By dropping these copolymer dispersion solutions below LCST to a quartz resonator surface and drying it, P(NIPAM-BMA) nanoparticle films were easily fabricated at room temperature. Surface observation of the nanoparticles film with SEM revealed that nanoparticles had the diameter of ~200 nm for both copolymers. Adsorption/desorption characteristics of the fabricated nanoparticle films were measured. The adsorption amount of the NIPAM-BMA copolymers to HCl gas was proportional to the loading amount on the resonator surface. The adsorption amount for the copolymer (20:1) was larger than that for the copolymer (10:1) and homopolymer, however, the recovery characteristic for the copolymer (20:1) was slightly poor compared with that of other polymers.

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清山賞受賞講演2

揮発性有機化合物の高感度検知を目指した
固体電解質および半導体型ガスセンサの開発

熊大院先端科学研究部

○木田 徹也

DEVELOPMENT OF SOLID ELECTROLYTE AND SEMICONDUCTOR GAS SENSORS
FOR HIGHLY SENSITIVE DETECTION OF VOLATILE ORGANIC COMPOUNDS

Tetsuya KIDA

Faculty of Advanced Science and Technology, Kumamoto University, Kumamoto 860-8555

The development of compact sensors that can detect volatile organic compounds (VOCs) is extremely important to address air quality issues and to avoid health risk associated with VOCs. This article reviews our recent progress in developing solid electrolyte and semiconductor gas sensors for highly sensitive detection of VOCs. An overview of the exploration of a mixed-potential-type VOC sensor using oxide ion-conducting BiCuVOx (Bi2Cu0.1V0.9O5.35) fitted with perovskite oxide electrodes is provided. The importance of designing its electrode structure is discussed. Other successful development of ultrasensitive VOC sensors using SnO2 nanocrystals and nanorods is also introduced.

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28.

アパタイト型ケイ酸ランタンを用いた
接触燃焼式エタノールガスセンサ

阪大院工

○中谷遥、田村真治、今中信人

CATALYTIC COMBUSTION-TYPE ETHANOL GAS SENSOR EMPLOYING
THE APATITE-TYPE OXIDE BASED CATALYST

Haruka NAKATANI, Shinji TAMURA, and Nobuhito IMANAKA

Graduate School of Engineering, Osaka University,
Suita-shi, Osaka 565-0871

A new catalytic combustion-type ethanol (C2H5OH) gas sensor was devised by applying Pt-loaded La10Si5.7Co0.3O27 solid solution as the C2H5OH oxidizing catalyst. The sensor showed the superior sensing performance of smooth and rapid response to C2H5OH gas at 150°C. Moreover, since the present sensor exhibited linear response to the C2H5OH gas concentration change, it is expected that the sensor can detect C2H5OH quantitatively at the operating temperature of 150°C.

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29.

吸着燃焼式マイクロガスセンサのVOC検知メカニズム:
触媒材料のVOC酸化活性に基づく検討

長崎大院工

○兵頭健生、日浦 健、永江和徳、鎌田 海、上田太郎、清水康博

VOC-SENSING MECHANISM OF ADSORPTION/COMBUSTION-TYPE GAS SENSORS:
INVESTIGATION BASED ON VOC-OXIDATION ACTIVITY OF CATALYTIC MATERIALS

Takeo HYODO, Takeru HIURA, Kazunori NAGAE, Kai KAMADA, Taro UEDA,
and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

Catalytic combustion behavior of ethanol over mesoporous γ-alumina loaded with 10 wt% metal oxide (MO) and/or 1.0 wt% Pt (A/mp-Al2O3, A: 1Pt, 10MO, or 1Pt/10MO (MO: CeO2 or Bi2O3)) and temperature-programmed desorption (TPD) and temperature-programmed oxidation (TPO) characteristics of ethanol adsorbed on these mp-Al2O3-based catalysts have been investigated in this study, to clarify the ethanol-sensing mechanism as the adsorption/combustion-type gas sensors. The loading of only MO improved the ethanol conversion of mp-Al2O3. However, the magnitude of ethanol response of the 10MO/mp-Al2O3 sensors was quite small, probably because of extremely slow rate of ethanol oxidation at elevated temperatures (450°C). On the other hand, the co-loading of Pt and CeO2 onto mp-Al2O3 was the most effective in improving the oxidation activity of ethanol among all the sensor materials, and the large magnitude of ethanol response of the 1Pt/10CeO2/mp-Al2O3 sensor probably originates from the quite rapid rate of ethanol oxidation.

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30.

高感度を実現する自動車排ガスセンサ
- 電界効果型NOxセンサと表面弾性波型PMセンサ -

日立a、日立金属b

○笹子 佳孝a、 礒部 敦a、 小高 貴浩a
  小松 成亘a、 中村 洋平a、 山脇 大造a、 依田 智子a、 牛房 信之a
  吉川 晃平a、 沖代 賢次b、 杉山 雄太b

EXHAUST GAS SENSORS REALIZING HIGH SENSITIVITY
- FET-TYPE NOx SENSOR AND SAW-TYPE PM SENSOR -

Yoshitaka SASAGO a, Atsushi ISOBE a, Takahiro ODAKA a, Shigenobu KOMATSU a,
Yohei NAKAMURA a, Taizo YAMAWAKI a, Chiko YORITA a, Nobuyuki USHIFUSA a,
Kohei YOSHIKAWA a Kenji OKISHIRO b and Yuta SUGIYAMA b

a R&D Group, Hitachi, Ltd.,
Kokubunji-shi, Tokyo 185-8601
b Hitachi Metals, Ltd.,
Minato-ku, Tokyo 108-8224

We have developed SiC-FET-type NOx sensor and SAW-type PM sensor for exhaust gas. SiC-FET-type NOx sensor utilizes SiC substrate and the gate layer made of yttria-stabilized zirconia, nickel oxide and platinum. The gate layer has catalytic effect for NOx and therefore the threshold voltage of the FET is sensitive to NOx. NOx concentration in the exhaust gas can be derived from the threshold voltage shift of the sensor FET. By virtue of SiC and the gate materials, the sensor has heat resistance at least up to 900ºC. SAW-type PM sensor utilizes langasite substrate and platinum electrode. The quantity of the PM adhering to the SAW device can be estimated from the frequency shift or delay time shift of SAW device. The PM sensor also has strong heat resistance and suitable for exhaust gas. The sensing principle of the FET and the SAW sensors make it possible to realize higher sensitivity compared to the conventional sensors.

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31.

排気ガスデバイス材料の高温ガス環境評価

日立a、日立金属b

○依田 智子a、 牛房 信之a、 笹子 佳孝a、 礒部 敦a、 沖代 賢次b、 杉山 雄太b

EXHAUST GAS SENSOR DEVICE MATERIALS TEST
UNDER HIGH TEMPARATURE GAS ATMOSPHERE

Chiko YORITA a, Nobuyuki USHIFUSA a, Yoshitaka SASAGO a
Atsushi ISOBE a, Kenji OKISHIRO b and Yuta SUGIYAMA b

a R&D Group, Hitachi, Ltd.,
Yokohama-shi, Kanagawa 244-0817
b Hitachi Metals, Ltd.,
Minato-ku, Tokyo 108-8224

We have developed SiC-FET-type NOx sensor and SAW-type PM sensor for exhaust gas. SiC-FET-type NOx sensor utilizes SiC substrate and SAW-type PM sensor utilizes langasite substrate. Both sensor electrode made of titanium layer and platinum layer. By Au stud bump on the top of platinum electrodes,SiC-substrate NOx sensor and langasite- substrate PM sensor connect ceramics substrate electrodes to assemble into an exhaust gas sensor module. The sensor modules need heat resistance at least up to 700 ºC, and also need strong gas corrosion resistance at 700ºC gas atmosphere. Joint material of those sensors has good resistance for non-methane organic gas, SO2, NO, NO2, SO2/NO/NO2  mixed gas at 700ºC. The novel metal joint materials, Pt metallize and Au stud bump, connect SiC and langasite to ceramic substrates realize excelling connecting as a high temperature corrosion gas resistance sensor.

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32.

原子力発電所格納容器内への酸素濃度計の適応(2)

東芝エネルギーシステムズ(株)a、b(株)東芝c,d

○柳生基茂a、金村祥平a、岡崎 幸基b、 長田 憲和c
高橋 愛実b、羽生 大仁b、伊藤 敏明b、山華 雅司d

ADAPTATION OF OXYGEN SENSOR INTO CONTAINMENT VESSEL OF NUCLEAR
POWER PLANT(2)

Motoshige YAGYUa, Shohei KANAMURAa, Koki OKAZAKIb, Norikazu OSADAb,
  Megumi TAKAHASHIb, Kisato HABUb, Toshiaki ITOb, Masashi YAMAGEd

aToshiba Energy Systems & Solutions Corp.
4-1, Ukishima-cho, Kawasaki-ku, Kawasaki 210-0862
bToshiba Energy Systems & Solutions Corp.
8, Shinsugita-cho, Isogo-ku, Yokohama 235-0032
cToshiba Corp.
2-4, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045
dToshiba Corp.
33, Shinsugita-cho, Isogo-ku, Yokohama 235-0032

Since the accident of Fukushima Daiichi Nuclear Power Station caused by the Great East Japan Earthquake, higher safety has been required for the nuclear power plants. In order to prevent hydrogen explosion, we need to monitor the oxygen concentration directly in the Primary Containment Vessel (PCV) environmental condition during the severe accident. We considered that the limiting current type oxygen sensor is promising as a direct measurement device that can be used under the condition inside the PCV at the severe accident. We confirmed a possibility that the limiting current type oxygen sensor that used gold for the electrode material could be adapted for the PCV in case of a severe accident.1) In order to improve the adaptability during a severe accident, we examined the operating condition of the oxygen sensor with which any chemical species other than oxygen do not interfere in an oxygen sensor measurement result.

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33.

LaGaO3を電解質とした低温作動型
限界電流酸素センサ

九州大学統合新領域オートモーティブサイエンス専攻1、九州大学カーボン
ニュートラル・エネルギー国際研究所(I2CNER)2

○松本真都1、猪石篤2、高垣敦2、石原達己2

LIMITING-CURRENT TYPE OXYGEN SENSOR USING LaGaO3 AS ELECTROLYTE

Manato MATSUMOTO1, Atsushi INOISHI2, Atsushi TAKAGAKI2, Tatsumi ISHIHARA2

1) Graduate School of Integrated Frontier Sciences, Department of Automotive Science,
Kyusyu University,
Fukuoka-shi, Fukuoka 819-0395
2International Institute of Carbon Neutral Energy Research (I2CNER)
Kyusyu University,
 Fukuoka-shi, Fukuoka 819-0395

Limiting current type oxygen sensor is compact and detects oxygen in a wide concentration range1. However, this sensor is required to be operated at high temperature because of insufficient oxide ion conductivity in Y2O3 stabilized zirconia (YSZ) and so, low temperature operation of limiting current type sensor is required at present from low temperature of exhaust gas from engines. The objective of this study is to develop limiting current type sensor operating at decreased temperature for achieving the high selectivity. The limiting current type sensor was prepared by using LaGaO3 based oxide for electrolyte. For electrode, Co based oxide was mainly prepared by screen printing method. Al2O3 plate with pin hole, 0.2~0.5mm in diameter, was mainly used for oxygen diffusion layer. I-V curves of the prepared limiting current sensor using LaGaO3 electrolyte is observed at temperature from 300~500℃. With decreasing operating temperature, limiting current became smaller, however, limiting current was increased with increasing oxygen partial pressure. Therefore oxygen partial pressure can be estimated from limiting current. As a result, low operating temperature limiting current type oxygen sensor is realized by using LaGaO3 fast oxide ion conductor.

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34.

h-BN/Al2O3補助電極の起電力式
アンモニア検知特性

愛媛大学院理工

○川渕 貴史、板垣 吉晃、猿丸 英理、青野 宏通

DETECTION OF AMMONIA BY POTENTIOMETRIC SENSORS WITH HEXAGONAL
BORON NITRIDE/ ALUMINA AUXILIARY ELECTRODE

Takafumi KAWABUCHI, Yoshiteru ITAGAKI, Eri SARUMARU, Hiromichi AONO

Graduate School of Science and Engineering, Ehime University,
Matsuyama-shi, Ehime 790-8577

Liver and renal diseases increase in ammonia concentration in human blood. Along with this, the concentration of ammonia in expiration increases. Therefore, detection of ammonia in breath exhaled from a human has a large potential to be a noninvasive pathological diagnosis. In this study, potentiometric ammonia sensors were prepared based on yttria-stabilized-zirconia (YSZ) electrolyte and Pt electrode. In our previous result, covering the Pt sensing electrode with hexagonal boron nitride (h-BN) ball-milled increased EMF response. In this study, the h-BN was mixed with Al2O3 to yield h-BN dispersion, h-BN/Al2O3. Sensor response was tested for ammonia, acetone, ethanol and hydrogen. Acetone, ethanol and hydrogen showed good responses. On the other hand, ammonia showed remarkably small response. We concluded that using Al2O3 as an auxiliary electrode selectively decreased ammonia response.

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35.

酸化グラフェン-金属イオン複合膜を用いた
電気化学式ガスセンサ

熊大院自然a、熊大グローバル教育b

○新谷 雅貴a、 Aynul Sakinah Ahamad Fauzi a、Nur Laila Hamidah a
佐々木 満a、Armando T. Quitain b、木田 徹也a

ELECTROCHEMICAL GAS SENSOR USING A GRAPHENE OXIDE MEMBRANE
INTERCALATED WITH METAL CATIONS

Masataka SHINTANI a, Aynul Sakinah AHAMAD FAUZI a, Nur Laila HAMIDAH a,
Mitsuru SASAKI a, Armando T. QUITAIN b, Tetsuya KIDA a

a Graduate School of Science and Technology, Kumamoto University,
Kumamoto-shi, Kumamoto 860-8555
b Global College, Kumamoto University, Kumamoto-shi, Kumamoto 860-8555

Human breath from lever and renal disease patients are known to increase to 15 ppm or higher. Therefore, exhaled NH3 can be a biomarker in a non-invasive breath test for such diseases. We reported that the Pt sensing electrode covered with the bead-milled hexagonal boron nitride (h-BN) increased the EMF response. However, the smaller size (0.15 m) of h-BN than the bead-milled ones was not effective for EMF enhancement. The FT-IR observation of the bead-milled h-BN suggested the formation of B-OH group in the h-BN crystal by the mechanical cleavage of the B-N bond. We concluded that the formed B-OH groups acted as a Brønsted acid site for the ammonia adsorption.

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