Vol. 34, Supplement A (2018)

Proceedings of the 63th Chemical Sensor Symposium
Tokyo, March 9-11, 2018

 

Abstracts



1.

コロニーフィンガープリントの機械学習に基づく真正細菌の高精度判別

東京農工大a、マルコムb

○石小木曽淳a、杉山由依a、吉野知子a、前田義昌a、林 泰圭b、原田 学b、松永 是a、田中 剛a

HIGHLY ACCURATE DISCRIMINATION OF EUBACTERIUM SPECIES BASED ON
MACHINE LEARNING OF COLONY FINGERPRINT

Atsushi KOGISOa, Yui SUGIYAMAa, Tomoko YOSHINOa, Yoshiaki MAEDAa, Tae-kyu LIMb, Manabu HARADAb, Tadashi MATSUNAGAa, Tsuyoshi TANAKAa

a Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16,
Naka-cho, Koganei, Tokyo 184-8588,
b Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071

The microbial identification test is useful in various fields including food production. Conventional methods such as DNA sequencing and MALDI-TOF-MS are widely utilized, while these techniques required expensive equipment and well-trained operators. Recently, we have proposed an imaging-based method termed “colony fingerprinting” for discrimination of microorganisms. Colony images were obtained using two-dimensional image sensor, and discrimination parameters were extracted from the obtained images. In the previous study, we demonstrated closely related 5 Staphylococcus spp. based on the colony fingerprinting. In this study, we further expand target bacteria to 20 bacterial species in 9 genera. In order to accurately discriminate the bacterial species, we examined the appropriate machine learning methods for the analysis of the colony images of each bacterium.

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2.

微生物ゲノムDNAの検出に向けたシグナリングアレイの開発

東京農工大a、横河電機b

○石川万智a、田口朋之b、蓼沼 崇b、吉野知子a、前田義昌a、松永 是a、田中 剛a

DEVELOPMENT OF SIMPLE DETECTION METHOD OF BACTERIAL GENES BY
SIGNALING ARRAY PROBE

Machi ISHIKAWAa, Tomoyuki TAGUCHIb, Takashi TADENUMAb, Tomoko YOSHINOa, Yoshiaki MAEDAa, Tadashi MATSUNAGAa, Tsuyoshi TANAKAa

a Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16,
Naka-cho, Koganei, Tokyo 184-8588
b Yokogawa Electric Co., 2-9-32 Nakacho, Musashino, Tokyo 180-8750

Detection of microorganisms is crucial in a wide range of industries including food, cosmetics and pharmaceuticals. Microarray testing is one of the promising methods for this purpose, although it is time consuming and labor intensive. To address this issue, we established a novel microarray technology using the signaling array probe (SAP) which comprises of fluorescent probe and quench probe, and generates fluorescent signal only when target DNA exists. Thus far, DNA fragments amplified by PCR were used as target DNA on the SAP-based detection system. However, PCR is potentially inhibited by sample ingredients, and might result in narrowing the target microorganisms depending on the primer design. In the present study, we attempted to directly use genomic DNA on the SAP-based detection system without PCR amplification. As a result, we successfully demonstrated the detection of genomic DNA extracted from bacteria in the presence of contaminating DNA (λ DNA). This method would be a useful to detect microorganisms in a variety of applications without DNA amplification.

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3.

電気化学薄層セルを用いた微生物の呼吸活性評価

阪府大

○齊藤真希、石木健吾、椎木 弘、長岡 勉

EVALUATION OF RESPIRATORY ACTIVITY OF MICROORGANISMS
USING ELECTROCHEMICAL THIN-LAYER CELLS

Maki SAITO, Kengo ISHIKI, Hiroshi SHIIGI and Tsutomu NAGAOKA

Department of Applied Chemistry, Osaka Prefecture University, Department of
Sakai, Osaka 599-8570

In this study, facultative anaerobic bacteria such as Escherichia coli (E. coli), Pseudomonas aeruginosa,and Shewanella oneidensis were straightforwardly immobilized into the conducting polypyrrole (PPy) by electrochemical deposition. PPy plays an important role as a substrate for entrapping bacteria because of the resulting density, viability, and distribution. Proliferative process of E. coli cells on the PPy film was monitored by using a dark-field microscope. The cell density of E. coli on the PPy film increased logarithmically from 5.0×105 cells cm-2 to 4.5×106 cells cm-2 after incubation for 12 h under aerobic condition. During the incubation, a formation and growth of biofilm were observed clearly on the PPy film. The respiratory activity of bacteria doped on the polymer film was evaluated by cyclic voltammetry in a thin-layer cell. The cell allowed to evaluate bacterial respiratory clearly regardless of anaerobic and aerobic conditions. This suggested that the developed platform can serve as a simple but powerful analytical tool to monitor bacterial activity.

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4.

ホルマザンの電気化学特性に基づいた細菌検出

阪府大学

○石木健吾、森下 綾、椎木 弘、長岡 勉

BACTERIAL DETECTION BASE ON THE ELECTROCHEMICAL
CHARACTERISTIC OF FORMAZAN

Kengo ISHIKI, Aya MORISHITA, Hiroshi SHIIGI and Tsutomu NAGAOKA

Department of Applied Chemistry, Osaka Prefecture University, Department of
Sakai, Osaka 599-8570

In this study, an electrochemical microbe detection technique was developed using a tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which was converted to an insoluble and redox active formazan compound in viable microbial cells. The insolubility of this formazan was effectively exploited as a surface-confined redox event; an indium-tin-oxide electrode, applying a microbial suspension, which had been incubated with MTT, was dried by heating for the extraction and adsorption of the formazan, leading to the appearance of a distinctive voltammetric oxidation peak at +0.1 V vs Ag|AgCl, the magnitude of which was successfully correlated to the number of viable microbes in the suspension. Thus, the electrochemical detection of formazan was effectively coupled with the thermal lysis of microbes. It is also noteworthy that this lysis adsorption technique was highly selective to the hydrophobic formazan molecule due to the removal of hydrophilic cell components during equilibration in a phosphate buffer before voltammetric measurement. This technique was capable of detecting microbes above 102 cells mL–1 and only required the incubation time of 1 h. The results of this study indicate that the sensitivity of the present technique is up to 1,000-fold higher than that of MTT colorimetry. The higher sensitivity was mainly ascribed to the concentration of the microbially produced formazan on the electrode by thorough desiccation of the bacterial suspension.

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5.

バイオLSIを用いたゼブラフィッシュ胚の酸素消費量に基づく毒性評価法の開発

兵庫県大a、日本航空電子工業b、東北大院環境科学c、東北大院工d

○寺尾和輝a、國方亮太b、須田篤史b、井上(安田) 久美c、伊野浩介d、 末永智一c、安川智之a

EVALUATION SYSTEM FOR TOXICITY OF CHEMICAL SUBSTANCES
BASED ON THE MONITORING OF OXYGEN CONSUMPTION
OF ZEBRAFISH EMBRYOS BY BIO−LSI

Kazuki TERAOa, Ryota KUNIKATAb, Atsushi SUDAb, Kumi Y. INOUEc,
Kosuke INOd, Tomokazu MATSUEc, Tomoyuki YASUKAWAa

a Graduate School of Material Science University of Hyogo, Ako, Hyogo 678-1297
b Japan Aviation Electronics Industry, Shibuya, Tokyo 150-0043
c Graduate School of Environmental Science Tohoku University, Sendai, Miyagi 980-0845
d Graduate School of Engineering Tohoku University, Sendai, Miyagi 980-0845

We have developed a rapid estimation method of the effect of chemicals to an ecological system based on the electrochemical detection of the respiration activity of zebrafish (Danio rerio) embryos. Zebrafish embryos were placed on a tip of large scale integration based amperometric sensor (Bio-LSI), which can be simultaneously detected the current responses of 400 microelectrodes. Images of the respiration activities were monitored based on the oxygen reduction currents at all 400 microelectrodes. The regions with low reduction currents in images coincided with the location of embryos in an optical image. The reduction currents around embryos decreased with introducing the artificial sea water containing 8 M valinomycin. The result indicates that the addition of valinomycin gave rise to the decrease of oxygen concentration around the embryos by increasing the respiration activity. The reduction currents of oxygen around embryos can be monitored by the bio-LSI system, even though the embryos moved on the tip. Moreover, the decreased levels of the reduction current of oxygen increased with increasing the concentration of valinomycin. There, we could estimate the effect of chemicals by the bio-LSI based monitoring system of the oxygen reduction currents of zebrafish embryos.

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6.

高感度・微量涙糖センサの開発

PROVIGATE

○片山憲和、宮澤雄弥、柳本吉之、加治佐平、伊藤成史

DEVELOPMENT OF TEAR GLUCOSE SENSORS WITH HIGH-SENSITIVITY AND
CAPABILITY OF DETECTING A TRACE AMOUNT OF SAMPLES

Norikazu KATAYAMA, Yuuya MIYAZAWA, Yoshiyuki YANAGIMOTO, Taira KAJISA and Narushi ITO

PROVIGATE Inc., University of Tokyo Entrepreneur Plaza, 7-3-1 Hongo Bunkyo-ku,
Tokyo 113-0033

Tear is one of the attractive body fluids for simple and non-invasive sampling, and tear glucose may provide the possibility as a new biomarker for indirectly monitoring blood glucose in diabetes. However, it is difficult to determine tear glucose on chips for point-of-care testing and/or self-monitoring, because the concentration of the glucose in tears is lower than that in blood serum. Here, we developed glucose oxidase (GOD) sensors to monitor glucose in tear fluids with high sensitivity using a trace amount of a samples. Amperometric continuous monitoring was performed to detect tear glucose levels using GOD immobilized membrane on a platinum and Ag/AgCl electrode. We confirmed that responses to glucose in buffer solutions (pH = 7.0) on the sensors increased with that concentrations for 14 to 1110 μM. Then, responses to tear fluids collected directly from healthy subjects were highly correlated with tear glucose levels measured by HPLC methods (R = 0.996). These results indicate that our sensors exhibited high sensitivity and accuracy to detect tear glucose levels using a trace amount of a samples and may provide a non-invasive and simple method available for early diagnosis and glycemic management.

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7.

カーボンフェルトへの酵素の化学修飾とフロー型バイオセンサへの応用

埼玉工大a、遼寧科技大b

○長谷部靖a、王 月b

CHEMICAL MODIFICATION OF ENZYMES ONTO CARBON-FELT
AND ITS APPLICATION TO FLOW-TYPE BIOSENSORS

Yasushi HASEBEa, and Yue WANGb

a Department of Life Science and Green Chemistry, Saitama Institute of Technology
Fukaya-shi, Saitama 369-0293, Japan
b School of Chemical Engineering, University of Science and Technology, LiaoNing,
Anshan-shi, LiaoNing 114501 China

Glucose oxidase (GOx) and horseradish peroxidase (HRP) were covalently immobilized on a porous carbon felt (CF) by using cyanuric chloride (CC) as a linking reagent. The resulting GOx-modified-CF (GOx-ccCF) was used as column-type enzyme reactor and placed on up-stream of the HRP-modified-CF(HRP-ccCF)-based H2O2 flow-detector to fabricate amperometric flow-biosensor for glucose. The chemical modification of the GOx via CC was effective to obtain larger catalytic activity as compared with the physical adsorption. Under the optimized conditions, highly selective and quite reproducible peak current responses toward glucose were obtained: the RSD for 30 consecutive injections of 3 mM glucose was 1.04%, and no serious interferences were observed for fructose, ethanol, uric acid, urea and tartaric acid for the amperometric measurements of glucose. The magnitude of the cathodic peak currents for glucose was linear up to 5 mM (sensitivity, 6.38 ± 0.32 µA/µM) with the limit detection of 9.4 µM (S/N=3, noise level, 20 nA). The present GOx-ccCF-reactor and HRP-ccCF-detector-coupled flow-glucose biosensor was utilized for the determination of glucose in beverages and liquors, and the analytical results by the sensor were in fairly good agreement with those by the conventional spectrophotometry.

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8.

尿中タンパク検出のためのマルチ酵素カスケードを利用した電気化学センサ

東北大

◯三浦 翼、井上(安田)久美、末永智一

ELECTROCHEMICAL SENSOR USING MULTI-ENZYME CASCADE FOR DETECTION
OF PROTEIN IN URINE

Tsubasa MIURA, Kumi. Y. INOUE and Tomokazu MATSUE

Graduate School of Environmental Studies,Tohoku University, Sendai-shi, Miyagi 980-8579

A daily urine protein test helps early detection of kidney diseases. But present urinalysis is not adequate for daily use because it needs human intervention. To rearise unobtrusive daily urine sensing in toilet, we have developed an electrochemical urine protein sensor repeatedly usable without addition of reagents by detecting glutamate generated from protein hydrolyzed by glutamate oxidase immobilized on a Prussian blue modified electrode. In this study, we investigated the use of multi-enzyme cascade reaction of proteinase K and carboxypeptidase A to promote the sensitivity by efficient hydrolysis of protein. We achieved a result that 0.3 mg/ml of albumin, which is necessary for screening for kidney disease, can be detected within 60 min, by hydrolyzed with mixture solution of proteinase K and carboxypeptidase A.

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受賞講演

分子認識能を賦与した有機薄膜トランジスタ型化学センサの創製

東大

○南 豪

CHEMICAL SENSORS ON THE BASIS OF ORGANIC THIN-FILM TRANSISTORS
FUNCTIONALIZED WITH MOLECULAR RECOGNITION MATERIALS

Tsuyoshi MINAMI

Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505

Organic thin-film transistors (OTFTs) have attractive features such as their mechanical flexibility and controllability of the electrical functions of π-conjugated molecules. I strongly believe that OTFTs are some of more suitable platforms to develop on-site chemical sensors for environmental pollutants or biomarkers, owing to their low-manufacturing costs and easy fabrication processes. In this regard, I have developed extended-gate type OTFT-based chemical sensors, which are modified with molecular recognition materials (i.e. antibodies, enzymes, or artificial receptors) to detect various target analytes. My preliminary results indicate that OTFT-based chemical sensors will be powerful and practical tools for electroanalytical chemists in the near future.

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9.

ヘパリンを鋳型とした分子インプリント高分子ナノ粒子の特異結合に伴う粒径変化と、
同高分子薄膜固定電極のヘパリン応答との相関

芝浦工大

○吉見靖男、追野大智、山口莉奈、関 真希

ANALYSIS OF TEMPLATE-SENSITIVITY IN THE SIZE OF MOLECULAR IMPRINTED
POLYMER NANOPARTICLE IN ORDER TO SPECULATE THE SENSING
MECHANISM OF ELECTRODES GRAFTED WITH MOLECULARLY IMPRINTED
POLYMERS

Yasuo YOSHIMI, Daichi OINO, Rina YAMAGUCHI and Maki SEKI
Department of Applied Chemistry, Shibaura Institute of Technology, Toyosu, Koto-ku, Tokyo 135-8548

Nanoparticles of molecularly imprinted polymer (MIP-NP) were synthesized with a template of unfractionated heparin (UFH) or low molecular weight heparin (LMWH) immobilized on the surface of glass beads. The diameters of MIP-NPs were increased by the both types of heparin. But the sizes were more highly sensitive to the template than to the other type of heparin. It indicates that polymer matrix of the MIP is expanded by the specific interaction between the template and MIP. The phenomena is probably a key factor of the sensing mechanism of the MIP-grafted electrode using change in redox current by the specific interaction.

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10.

陽極酸化アルミナ由来のナノ構造を用いた
高感度QCMセンサの作製とバイオセンサへの応用

関西大

○浅井直人、清水智弘、新宮原正三、伊藤 健

FABRICATING A HIGHLY SENSITIVE QCM SENSOR USING AAO
NANOSTRUCTURE ITS APPLICATION FOR BIOSENSOR

Naoto ASAI, Tomohiro SHIMIZU, Shoso SHINGUBARA, and Takeshi ITO

Kansai University, Suita-shi, Osaka 564-8680

We fabricated Anodic Aluminum Oxide (AAO) nanostructure on the surface of a QCM-based sensor to improve the sensitivity. The fabricated sensor improved the sensitivity, as one of examples, the antigen-antibody interaction was measured. The nanostructure composed of Al2O3 have the large surface area because of self-assembled nanoholes array with high aspect ratio. In addition, AAO nanostructure is controllable easily by changing the anodization conditions such as applying voltage, electrolyte, and anodization time. The sensitivity of the QCM-based sensor was improved due to enlarged surface area. In this study, the nanohole diameter was 60 nm and AAO thicknesses were ranged from 200 nm to 1000 nm. We compared the frequency shifts corresponding to the thickness of AAO nanostructure on the antigen-antibody interaction by using the fabricated QCM-based sensor. The detection limit was improved from 0.48 µg/ml to 0.20 µg/ml comparing with Au thin film and AAO nanostructured QCM-based sensor.

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11.

二次免疫反応を利用した表面プラズモン共鳴バイオセンサ

北大院国際食資源a、北大国際連携研究教育局b、ラージシャヒ大学c、ウシオ電機d

○川口俊一a,b、カビラズ デュラルc、森田金市d、高橋昌志a,b

SURFACE PLASMON RESONANCE IMMUNOSENSOR
USING SECONDARY IMMUNOREACTION

Toshikazu KAWAGUCHIa,b, Dulal KABIRAZc, Kinichi MORITAd, Masashi TAKAHASHIa,b

a Graduate School of Global Food Resources, Hokkaido University,
Sapporo-shi, Hokkaido 060-8589
b Global Institution for Collaborative Research and Education,
Hokkaido University,Sapporo-shi, Hokkaido 060-8589
c Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh, 6205
d USHIO INC. Tokyo 100-8150

We have developed a practical Surface Plasmon Resonance (SPR) Immunosensor for detection of beta agonists. Because beta agonist is a small molecule, Indirect competitive inhibition immunoassay is employed. However, the SPR signal response is very small because SPR monitors the mass transfer at the interface in the initial stage of immunoreaction. Therefore, the amplification method needs to be developed for a practical use. In this research, the signal amplification using secondary immunoreaction with indirect competitive inhibition immunoassay is studied. From the basis of kinetic discussion, it was noticed that the affinity constants determine the sensitivity of immunosensor. To concrete this concept, the laid down and standing structure of analyte/antibody were compared. In the first immunoreaction of indirect competitive inhibition immunoassay, the laid down structure of antigen analog immobilized on the sensor surface shows a higher affinity constant than standing structure. In contrast, the standing structure of antibody shows a higher affinity constant than the laid down structure. It indicates the recognition part of antibody. The first antibody recognizes a tail of beta agonist, and the secondary antibody binds to Fc part of the first antibody. The structure was estimated by XPS, EC, and STM.

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12.

ポリマー圧電素子を用いた病原体特異的分子検出用
バイオセンサの開発
-分子識別素子の設計-

東京都市大a、北海道大学人獣共通感染症リサーチセンタb

○武田真理子a、平田孝道a、黒岩 崇a、中島千絵b、 鈴木定彦b、宗像文男a

DEVELOPMENT OF BIOSENSOR USING PIEZOELECTRIC POLYMER FOR
PATHOGEN SPECIFIC MOLECULAR DETECTION
- BIORECEPTOR ELEMENT DESIGN -

Mariko TAKEDAa, Takamichi HIRATAa, Takashi KUROIWAa, Chie NAKAJIMAb,
Yasuhiko SUZUKIb and Fumio MUNAKATAa

a Tokyo City University,
Setagaya-ku, Tokyo 158-8557
b Research Center for Zoonosis Control, Hokkaido University,
Kita-ku, Sapporo 001-0020

The conventional method for the diagnosis of infective diseases is using a biosensor with a quartz crystal. The mass sensor with the quartz crystal is monitoring mass changes of targets in high frequency. However, the sensor with the quartz crystal does not response to the vibration in the range of high frequency depend on molecular size of polymer materials. In this report, we present a new sensor using a piezoelectric polymer so called PolyVinylidene DiFluoride (PVDF). This sensor is covered with polyimide tape because the tape is possible to use in chemical solution. The tape was modificated by plasma-ion irradiation method to generate functional group (-COOH) on the surface of the polyimide tape. The results indicate that the immobilized host material on the surface of the polyimide tape was able to detect the target (guest) material.

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13.

サブフェムトモル濃度のタンパク質を検出可能な
有機トランジスタ型センサの開発

東大a、山形大b

南木 創a、佐々木由比a、時任静士b、南 豪a

DEVELOPMENT OF AN ORGANIC TRANSISTOR-BASED SENSOR FOR DETECTING
PROTEINS WITH SUB-FEMTOMOLAR SENSITIVITY

Tsukuru MINAMIKIa, Yui SASAKIa, Shizuo TOKITOb, Tsuyoshi MINAMIa

a Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505
b Graduate School of Science and Engineering, Yamagata University,
Yonezawa, Yamagata 992-8510

There is a growing interest in achieving sensor systems to enable on-site testing of biomarkers. Herein, a new strategy for highly sensitive protein detection at sub-femtomolar levels without any labelling processes has been demonstrated by using an organic thin-film transistor (OTFT). An artificial histidine-rich protein receptor (nickel(II)-nitrilotriacetic acid complex, Ni(II)-nta) functionalizes a detection portion (i.e. an extended-gate electrode) of the fabricated OTFT device. The OTFT-based sensor responds electrically and selectively to a target analyte (serum albumin), meaning that the binding processes at the Ni(II)-nta on the extended-gate electrode for the analyte affect the field-effect properties of the device. Our results demonstrate that the combination of the OTFT with the artificial receptor is an ideal approach for label-free and immune-free protein detection.

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14.

A VALVE-LESS MICROFLUIDIC DEVICE WITH A PUSH-PULL SEQUENTIAL SOLUTION
EXCHANGE FUNCTION FOR FLUORESCENCE IMMUNOASSAY

筑波大

Shishir Kanti PRAMANIK and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8573

A simple microfluidic device was developed for the detection of proteins. Efficient exchange of solutions was realized by introducing a solution into the reaction chamber and subsequently returning the solution to its reservoir one by one. The influence of hydrophobicity of the reaction chamber and viscosity of a blocking solution on solution exchange was examined. Analytical performance of the device was demonstrated by detecting human interleukin 2 (IL-2) by sandwich immunoassay. Clear dependence of fluorescence intensity on IL-2 concentration was observed in a range between 125 pg/mL and 2.0 ng/mL and the detection limit was 105 pg/mL.

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15.

化学修飾した炭素繊維に固定化したアルカリ
ホスファターゼを用いる亜鉛(II)イオンのフロー計測

神奈川工科大

○佐藤生男、高橋佑、神崎 愷

FLOW MEASUREMENT USING ALKALINE PHOSPATASE IMMOBILIZED ONTO
CARBON FIBERS CHEMICALLY MODIFIED

Ikuo SATOH,Yu TAKAHASHI, and Yasushi KANZAKI

Department of Applied Chemistry, Faculty of Engineering,
Kanagawa Institute of Technology,
1030 Shimo-Ogino, Atsugi-shi, Kanagawa, 243-0292, Japan

Use of the immobilized alkaline phosphatase onto carbon fibers chemically treated was applied to a flow-injection determination of zinc(II) ions. The fibers were packed into a plastic column(1.5 mL) and then,this column was set into a flow- injection system. The system was applied to a microdetermination of zinc(II) ions based on an apoenzyme reactivation method. The performance characteristics of the flow system were reported.

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17.

ストレス計測評価用バイオセンシングデバイスの研究開発(VI)
唾液硝酸イオン計測による緊張ストレス評価の検討

産総研・阪大先端フォトバイオa、神大院人間発達b、阪大院工c、神大院海事d、阪工大工e

○脇田慎一a,b,c、北村健一a,d、村井康二d、大﨑脩仁a,b、金時卓哉a,e、森内隆代e

R & D OF BIOSENSING DEVICES FOR STRESS MONITORING(VI)
STUDY ON EVALUATION FOR ACUTE STRESS USING SALIVARY NITRATE MONITORING

Shin-ichi WAKIDAa,b,c, Kenichi KITAMURAa,d, Koji MURAId, Shuto OSAKIa,b, Takuya KINTOKIa,e, Takayo MORIUCHIe

a AIST PhotoBIO-OIL, National Institute of Advanced Industrial Science and Technology (AIST),
2-1 Yamada-Oka, Suita, Osaka 565-0871
b Graduate School of Human Development and Environment, Kobe University
3-11 Tsurukabuto, Nada-ku, Kobe 657-8501
c Graduate School of Engineer, Osaka University, 2-1 Yamada-Oka, Suita, Osaka 565-0871
d Graduate School of Maritime Sciences, Kobe University,
5-1-1 Fukae-minami, Higashinada, Kobe 658-0022
e Faculty of Engineering, Osaka Institute of Technology,
5-16-1 Omiya, Asahi-ku, Osaka 535-8585

To develop on new stress-sensing devices for stress science study, we have investigated ISFET (ion-selective field-effect transistor) based biosensors for measurement of several salivary stress-marker candidates and also have studied organic semiconductor based ISFETs, OFET based biosensors. During the fundamental studies on the prototype of stress-sensing devices, such as electrophoretic microfluidics and ISFETs for real salivary samples of healthy volunteers, we found salivary nitrate will be a biomarker candidate on the autonomic nervous system response to stress. To make a scientific study on the acute stress response mechanism of salivary nitrate, we investigated simultaneous monitoring for healthy volunteers of a ship handling using wearable heart rate monitor and salivary nitrate sampling measurement by prototype of salivary nitrate checkers based on NO3--ISFETs using direct potentiometry. We will introduce the several results of acute stress subject-experiments for healthy volunteers from the point of view from the behavior of salivary nitrate for mental workload of events.

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18.

液体金属とイオン液体を利用したマイクロ流体環境センサの開発

横国大工学研究院

○太田裕貴

MICROFLUIDIC ENVIRONMENT SENSOR USING LIQUID METAL AND IONIC LIQUID

Hiroki OTA

Department of Systems integration, Yokohama National University,
79-5 Tokiwadai, Hodogaya-ku, Yokohama, 240-8510

Flexible devices have many potential applications in wearable electronics, robotics, health monitoring, and more. Mechanically deformable devices and sensors such as flexible devices enable conformal coverage of electronic systems on curved and soft surfaces. Sensors utilizing liquids confined in soft templates as the sensing component present the ideal platform for such applications, as liquids are inherently more deformable than solids. Here we demonstrate microfluidic environmental sensors using liquid metal, highly sensitive pressure sensor based on tactile diaphragm pressure sensor, and temperature/humidity/oxygen sensors with mechanically robust liquid-liquid “heterojunction”. This report will present an important advancement towards the realization of liquid-state electronic systems

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19.

金属置換型ク−ロメトリ−の高感度化

筑波大

○アンソリ イサ、滝江 秀一、鈴木 博章

IMPROVEMENT OF COULOMETRIC DETECTION COUPLED WITH METALLIZATION

Isa ANSHORI, Shuichi TAKIE and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8573

To improve detection sensitivity of a microdevice based on coulometry coupled with metallization, a metal wire was used to connect two flow channels to cause redox reactions as an alternative to a liquid junction. Three methods were tested. First, a wire with Ag/AgCl on both ends was used. Next, to increase overpotential, one end of the wire was replaced with nickel or zinc. Alternatively, potential difference was applied directly to solutions in the flow channels. Each of these methods had an effect and the charge or the amount of silver deposited on the platinum electrode could be increased by increasing the potential difference between the solutions in the two flow channels. With the third method, the achieved lower detection limit for H2O2 was 2.4 nM (3σ).

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20.

モリブデン金属を基盤とするリン酸イオンセンサの 開発と設計

京大

○徐 克彬、白井 理、北隅優希、加納健司

DESIGN OF PHOSPHATE ION SENSOR USING MODIFIED MOLYBDENUM ELECTRODE

Kebin XU, Osamu SHIRAI, Yuki KITAZUMI and Kenji KANO

Graduate School of Agricultural, Kyoto University,
Kyoto-shi, Kyoto 606-8505

Phosphorous is one of three major nutritional elements for plants and usually exists as phosphate in nature. For wastewater treatment, the development of a high-performance phosphate sensor would be very helpful. A novel phosphate ion-selective electrode was constructed using a molybdenum electrode coated by a molybdenum phosphate complex. The sensor exhibited a linear response to HPO42- in a concentration range from 1.0 × 10−5 to 1.0 × 10−1 mol L-1 with a slope of −29 mV dec-1. The response was unaffected by common anions in nature, such as chloride, carbonate, and sulfate. The electrode was stable for at least 4 weeks.

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21.

シリカゲルインクを用いた長期安定性および
即時使用性に優れた印刷型薄膜参照電極の作製と評価

東理大理工a、東理大総研院b

○古茂田将人a、星 芳直a、四反田功a,b、板垣昌幸a,b

FABRICATION AND CHARACTERIZATION OF SCREEN-PRINTED REFERENCE ELECTRODE WITH
LONG-TERM STABILITY AND INSTANT USABILITY USING SILICA GEL INK

Masato KOMODAa, Yoshinao HOSHIa, Isao SHITANDAa,b and Masayuki ITAGAKIa,b

a Department of Pure and Applied Chemistry, Faculty of Science,
Tokyo University of Science
Noda, Chiba 278-8510, Japan
b Research Institute of Science and Technology, Tokyo University of Science
Noda, Chiba 278-8510, Japan

In this research, we fabricated a screen-printed reference electrode using poly vinylidene di fluoride (PVdF) binder and silica gel. We newly prepared fine KCl particles of 15 m diameter, and changed component and preparing process of ink to improve a long-term stability of the reference electrode. The reference electrode indicated 2 minutes set-up time and 20 days lifetime. These performance were from the fine structure of liquid junction layer and electrolyte layer. Moreover, the inner structure of the reference electrode was analyzed by electrochemical impedance spectroscopy. Three semicircles and finite diffusion impedance appeared on the Nyquist plot. The parameters related to the lifetime and set-up time of the reference electrode were estimated by curve fitting using an equivalent circuit.

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22.

Close型バイポーラ電極による液絡不要の参照電極
集積型アンペロメトリックセンシングプローブの開発

東北大院環境a、東北大工b

〇井上(安田)久美a,b、シティ マストゥラa、都賀亮人b、伊藤―佐々木 隆広a、珠玖 仁a,b、末永智一a,b

AMPEROMETRIC SENSOR PROBE WITH LIQUID-JUNCTION FREE REFERENCE
ELECTRODE SYSTEM BASED ON CLOSED BIPOLAR ELECTRODE SYSTEM

Kumi Y. INOUEa,Siti MASTURAHa,Ryoto TSUGAb
Takahiro ITO-SASAKIa,Hitoshi SHIKUa,b and Tomokazu MATSUEa,b

a Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aoba, Aramaki,
Aoba, Sendai 980-8579
b School of Engineering, Tohoku University, 6-6-11 Aoba, Aramaki, Aoba, Sendai 980-8579,

A reference electrode used for electrochemical measurement generally has a liquid junction to allow the ion transport between an internal electrolyte and a test liquid for electrical conduction between the reference electrode and the test liquid. However, the liquid junction often causes troubles such as clogging and test liquid contamination. Previously, we have developed a chip type device integrating a reference electrode without liquid junction using a close bipolar electrode system. In this research, we fabricated a probe type amperometric sensor integrated with liquid-junction free reference electrode system based on the knowledge obtained by the previous study. This compact device demonstrated to be used for ferrocenemethanol quantification. We also examined the apparent equation potential of the voltammogram. From this examination, we found the method expected to lead the improvement of the stability of the reference potential of the probe type device.

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23.

金属フタロシアニン系薄膜電極の H2O2応答特性

九工大

○高瀬聡子、森山実加子、清水陽一

HYDROGEN PEROXIDE SENSOR WITH METAL-PHTAHLOCYANINE THIN-FILM ELECTRODES

Satoko TAKASE, Mikako MORIYAMA, and Youichi SHIMIZU

Department of Applied Chemistry, Kyushu Institute of Technology,
Tobata, Kitakyushu, Fukuoka 804-8550

Hydrogen peroxide is one of products for many enzymatic- and electrochemical- reactions, then simple and highly selective detection methods for H2O2 are strongly required. As some metal complex catalysts have high selective catalytic properties and chemical stability, we focused on the metal phthalocyanines (MPc) and their family for electrode material of hydrogen peroxide sensor. In this study, various MPc thin-films were prepared on ITO electrode by electrochemical and drop-coating methods, and their potentiometric and amperometric detection properties to H2O2 were evaluated. Among the tested MPc based sensors, the CoPc thin-film electrode showed good potentiometric response from micro molar concentration revel. In these sensor responses, an electrochemical 2-electron H2O2 reduction reaction was proposed. As for the amperometric sensing, the cobalt naphthalocyanine and the cobalt hexadeca-fluoro phthalocyanine-based sensors showed high sensitive amperometric responses to H2O2.

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特別講演1

電気化学インピーダンス法による電極構造
と電気化学反応機構の解析

東理大理工

○板垣昌幸

ANALYSES ON ELECTRODE STRUCTURE AND REACTION MECHANISMS BY
ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

Masayuki ITAGAKI

Faculty of Science and Technology, Tokyo University of Science,
Noda-shi, Chiba 278-8510

Electrochemical impedance spectroscopy (EIS) has been applied to evaluations of various electrochemical devices because time constants in electrode response can be discriminated by the measurement of transfer function in the wide frequency range. The application of EIS allows the information regarding electrode structure and electrochemical reaction mechanisms and enables highly sensitive detection of a sensor. In this presentation, the theory of EIS is introduced firstly. And some topics related to impedance analyses on electrochemical sensors are explained, for example, 1. Equivalent circuit and Faradaic impedance, 2. Impedance of porous structure, 3. Diffusion impedance of micro-electrode, 4. Sensing by resistmetry, 5. Corrosion sensor for infrastructure, and 6. 3D impedance to analyze battery electrode.

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特別講演2

半導体薄膜を用いた有機系浮遊微粒子およびガス、においセンサの研究

東京電機大工

○原 和裕

STUDY ON SENSORS FOR DETECTING AIRBORNE PARTICLES, GAS AND
SMELL USING SEMICONDUCTOR THIN-FILMS

Kazuhiro HARA

School of Engineering, Tokyo Denki University,
5 Senju-Asahicho, Adachi-ku, Tokyo 120-8551

This report describes three kinds of conductometric sensors using semiconductor thin-films. The first one is a sensor for detecting organic airborne particles such as pollen, mold spores, PM2.5 and house dust. The sensor is fabricated by MEMS technology. The sensing film is a multi-layered one composed of SnO2-based and Fe2O3-based films. The sensor resistance changes toward these particles depending on their sizes and constituents. The kind of particles can be identified from its resistance change. The second one is a sensor made of WO3-based multi-layered thin-films with interspaces. The sensor is selective and sensitive to H2 gas. The Ag addition to the sensing film increases the sensitivity and selectivity to NO gas, while the deposition of Pt nanoparticles on the sensing film enhances the sensitivity to NH3 gas. The third one is a sensor equipped with an adsorption/desorption layer made of activated carbon supported on a rotation mechanism. The sensor identifies isovaleric acid and ethanol gases from its resistance change.

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24.

希土類複合酸化物を用いた接触燃焼式ガスセンサのエチレン検出特性

阪大

〇田村真治、渡邉美寿貴、中谷 遥、今中信人

C2H4 SENSING PERFORMANCE OF CATALYTIC COMBUSTION-TYPE GAS SENSOR EMPLOYING
RARE EARTH COMPLEX OXIDE

Shinji TAMURA, Mizuki WATANABE, Haruka NAKATANI, and Nobuhito IMANAKA

Graduate School of Engineering, Osaka University,
Suita, Osaka 565-0871

A new catalytic combustion-type C2H4 gas sensor was fabricated by using Pt-loaded Ce0.76Zr0.19Zn0.05O1.95 (CZZn) solid solution as the C2H4 oxidizing catalyst. The sensor employing 10 wt.% Pt / CZZn catalyst responded to C2H4 gas smoothly and reproducibly with linear response to C2H4 gas concentration change at the temperature as low as 85 °C. The 50 % response time of the sensor at 85 °C was 2.0 s. The sensor also exhibited the high C2H4 selectivity without showing meaningful response to other VOCs. Furthermore, the present sensor was found to be able to detect C2H4 even in a wet atmosphere whose humidity was 90 %.

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25.

貴金属電極を用いた高性能ダイオード型水素センサ開発
 –Pt電極へのAuコーティングの影響–

長崎大

○大草俊郎、坂田 亘、上田太郎、鎌田 海、兵頭健生、清水康博

DEVELOPMENT OF HIGHLY SENSITIVE DIODE-TYPE HYDROGEN SENSORS USING
NOBLE-METAL ELECTRODES –EFFECTS OF Au COATING ON Pt ELECTRODES–

Toshiro OKUSA, Wataru SAKATA, Taro UEDA, Kai KAMADA, Takeo HYODO,
and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521

H2-sensing properties of diode-type gas sensors using an anodized TiO2 film and Pt electrodes coated with or without Au have been investigated, and the effects of Au coating on the Pt electrodes on the H2-sensing properties have been discussed in this study. The Au coating on the Pt electrode greatly improved the response to 8000 ppm H2 in dry air, to reduce the O2-concentration dependence of the H2 response. On the other hand, the Au coating on the Pt electrode largely reduced the catalytic combustion behavior of H2 over the Pt electrode in dry air. These results probably indicate that a decrease in the number of oxygen adsorbates on the electrode increased the number of dissociatively adsorbed and dissolved hydrogen species and then enhanced the H2 response. In addition, the addition of moisture into the target gas improved the H2 response in air, whereas it was ineffective in improving the H2 response in N2. They are probably because the water molecules adsorbed on the electrode surface interrupted the adsorption of oxygen molecules under the operation in air, leading to reduction of the H2-oxidation activity on the electrode.

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26.

In2O3半導体ガスセンサのセンサ応答における
酸素吸着種と感度の相関関係

九大a、Taiyuan University of Technologyb

○末松昂一a、孫 永嬌a,b、渡邉 賢a、西堀麻衣子a、島ノ江 憲剛a

RELATIONSHIP BETWEEN THE OXYGEN ADSORPTION SPECIES AND
THE SENSOR RESPONSE ON THE In2O3 SEMICONDUCTOR GAS SENSORS

Koichi SUEMATSUa, Yongjiao SUN b,c, Ken WATANABEa, Maiko NISHIBORI a, Kengo SHIMANOEa

a Department of Advanced Materials Science and Engineering, Faculty of Engineering
Sciences, Kyushu University, Kasuga, Fukuoka 816-8580, Japan.
b Department of Molecular and Materials Science and Engineering, Faclty of Engineering
Sciences, Kyushu University, Kasuga, Fukuoka 816-8580, Japan.
c Micro and Nano System Research Center, Key Lab of Advanced Transducers and
Intelligent Control System (Ministry of Education) & College of Information Engineering,
Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.

Oxygen adsorption state of Inn2O3 nanoparticles was investigated based on the depletion depth model. According to the relationship between oxygen partial pressure and the relative resistance, which is ratio of the electric resistance in atmosphere with and without oxygen (R/R0), it was found that the Inn2O3 nanoparticles are in the volume depletion state in dry and wet atmospheres. Additionally, oxygen adsorption equilibrium constants for O- and O2- were well related to the depletion depth (Rair/R0). The sensor responses to 8 ppm CO and H2 are also strongly attributed to the depletion depth in the Inn2O3 nanoparticles.

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27.

ZnO上へのAuナノ粒子担持による選択的トルエン検知

九大院総理工a、九大エネ基盤センターb

○末松昂一a、渡邊厚介b、島ノ江憲剛a

HIGHLY SELECTIVE TOLUENE DETECTION USING Au NANOPARTICLES
LOADED ZnO GAS SENSORS

Koichi SUEMATSUa, Kosuke WATANABEb, Kengo SHIMANOEa

a Department of Advanced Materials Science and Engineering, Faculty of Engineering
Sciences, Kyushu University, Kasuga, Fukuoka 816-8580, Japan.
b Research and Education Center for Advanced Energy Materials, Devices, and
Systems, Kyushu University, Kasuga, Fukuoka 816-8580, Japan.

In this study, Au nanoparticles were added on the ZnO nanoparticles using co-precipitation method to obtain the highly selective toluene sensor. Au loading amount was set at 0.15 mol%. Particle size of the Au nanoparticles on the ZnO was about 2 nm, and Au nanoparticles were highly dispersed. The sensor responses to 100 ppm ethanol, acetone, and toluene for ZnO NPs were 980, 1430, and 840, respectively, while those of Au-ZnO NPs were 7, 7, and 92, respectively. Such strong reduction on the sensor response seems to be caused by the catalytic oxidation on the surface of sensing films. Nevertheless, sensor response to toluene using Au-ZnO NPs was more than 10 times higher than those to ethanol and acetone. It is considered that catalytic activity as well as electronic sensitization gives high gas splitting effect, and allows to provide highly selective gas sensors.

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28.

紫外線照射下での酸化物半導体ガスセンサのVOC応答特性

長崎大

○兵頭 健生、青松俊伸、鎌田 海、上田太郎、清水康博

VOC-SENSING PROPERTIES OF OXIDE SEMICONDUCTOR SENSORS
OPERATED UNDER UV-LIGHT IRRADIATION

Takeo HYODO, Toshinobu AOMATSU, Kai KAMADA, Taro UEDA, and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University
1-14 Bunkyo-machi, Nagasaki 852-8521

Sensing properties of SnO2-based semiconductor gas sensors to acetone and ethanol have been investigated under UV-light irradiation (peak wavelength: 365 nm) at 30°C in dry air. The responses of a pristine SnO2 sensor showed small responses to both acetone and ethanol even under strong UV-light irradiation of 590 mW cm−2, but the loading of Pd onto SnO2 was effective in enhancing sensing properties to both gases. The acetone response was smaller than the ethanol response, but the response behavior was quite different between them. The mixture of ethanol into dry air definitely decreased the resistance of all the sensors, and the magnitude of ethanol response monotonically increased with an increase in the amount of Pd loaded onto SnO2. On the other hand, the response behavior to acetone was really dependent on the amounts of Pd loaded. The small amount of Pd loaded (0.05 and 0.1 wt%) especially resulted in an abrupt increase in the resistance in mixture of acetone into dry air and a subsequent intermittent decrease in the resistance in acetone balanced with dry air. This specific behavior probably indicated that the products of partial oxidation of acetone were negatively adsorbed on the Pd-loaded SnO2 surface first, and then they reacted with oxygen adsorbates.

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29.

不定比酸化タングステンのガス検知における役割

熊本大自然a、熊本大工b、熊本大先端c、熊本大パルスd

松本和也a、松本惇平b、○橋新 剛c,d、松田元秀c、久保田弘d

ROLE OF NONSTOICHIOMETRIC TUNGSTEN OXIDE IN GAS DETECTION

Kazuya MATSUMOTOa, Junpei MATSUMOTOb, Takeshi HASHISHINc,d,
Motohide MATSUDAc, and Hiroshi KUBOTAd

a Graduate School of Science and Technology, Kumamoto University,
2-39-1 Kurokami, Chuo-ku, Kumamoto-shi, 860-8555
b Faculty of Engineering, Kumamoto University,
2-39-1 Kurokami, Chuo-ku, Kumamoto-shi, 860-8555
c Faculty of Advanced Science and Technology, Kumamoto University,
2-39-1 Kurokami, Chuo-ku, Kumamoto-shi, 860-8555
d Institute of Pulse Power Science, Kumamoto University,
2-39-1 Kurokami, Chuo-ku, Kumamoto-shi, 860-8555

The role of nonstoichiometric tungsten oxide with the composition of WO2.72 and WO2.90 on gas detection was investigated by using interface length effect of interdigitated Au electrodes. The long interface length of 1.5 mm was used for amplifying sensor response to 1% hydrogen. The sensor responses (S) of WO2.72, WO2.90 and WO3 showed 24.3 4.0 and 2.2, respectively. Especially, the sensor response of WO2.72 was about ten times higher than that of WO3. The sensor response of WO2.72 with short interface length of 0.5 mm was 1.6. The sensor responses of WO2.72 per both interface lengths of 1.5 mm and 0.5 mm showed same value of 1.6 /mm, suggesting that this value was related to amplifying the sensor response by increasing interface length.

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30.

La2O3/SnO2積層型CO2センサにおけるLa2O3の成膜状態
がCO2応答に及ぼす影響

豊橋技科大

○大野 賢、岩田達哉、松田恭輔、高橋一浩、澤田和明

INFLUENCE OF DEPOSITION CONDITION OF La2O3 ON CO2 RESPONSE IN
La2O3/SnO2 STACKED CO2 SENSOR

Ken ONO, Tatsuya IWATA, Kyosuke MATSUDA, Kazuhiro TAKAHASHI, Kazuaki SAWADA

Toyohashi University of Technology, Toyohashi-shi, Aichi 441-8122

In this study, the CO2 sensors based on La2O3/SnO2 stacked structures were fabricated with controlling the of deposition condition of the La2O3-sensitization layer. The La2O3 and a 100-nm-thick SnO2 sensing layer were deposited on Pt inter-digitated electrodes. A precursor of La2O3 was spin-coated with the rotational velocity in the range of 1000 - 2000 rpm. The sensing area was 110 × 110 μm2. CO2 response of the devices changed with the rotational speed for spin-coating of the La2O3. From SEM and AES analyses, it was revealed that the La2O3 has porous structure and it was found that CO2 adsorption on La2O3 itself is linked to the CO2 response.

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31.

MEMS型SnO2センサを用いたガス検知の高感度化

九大

○原野 航、末松昂一、渡邉 賢、西堀麻衣子、島ノ江憲剛

HIGHLY SENSITIVE MEMS-TYPE SnO2 GAS SENSORS
USING NEWLY DETECTION APPROACH

Wataru HARANOa, Koichi SUEMATSUb, Ken WATANABEb,
Maiko NISHIBORIb, Kengo SHIMANOEb

a Department of Molecular and Material Sciences, Interdisciplinary Graduate School of
Engineering Sciences, Kyushu University, Kasuga, Fukuoka 816-8580, Japan
b Department of Advanced Materials Science and Engineering, Faculty of Engineering
Sciences, Kyushu University, Kasuga, Fukuoka 816-8580, Japan

In this study, we tried to improve the sensor response of the MEMS-type gas sensor to ethanol using pulse type heating and its specific sensing characteristics. Thin (10 μm) and thick (50 μm) SnO2 sensing films were prepared on the MEMS device, and their sensor response to ethanol and hydrogen were measured based on the two types of the determination on the sensor response; one is the sensor response at initial term in pulse heating (Si) and another is the sensor response at end term in pulse heating (Se). As a result, Si was strongly higher than Se, especially in the response to ethanol regardless of the thickness of sensing film. Additionally, high sensor response value, Si, was achieved using the thick SnO2 sensor not only to the ethanol but also to hydrogen.

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32.

原子力発電所格納容器内への酸素濃度計の適応

東芝エネルギーシステムズ

○柳生基茂a、岡崎幸基b、田中元気b
  羽生大仁b、高橋愛実b

ADAPTATION OF OXYGEN SENSOR INTO CONTAINMENT VESSEL OF NUCLEAR
POWER PLANT

Motoshige YAGYUa, Koki OKAZAKIb, Genki TANAKAb, Kisato HABUb, Megumi TAKAHASHIb

a Toshiba Energy Systems & Solutions Corp.
4-1, Ukishima-cho, Kawasaki-ku, Kawasaki 210-0862
b Toshiba Energy Systems & Solutions Corp.
8, Shinsugita-cho, Isogo-ku, Yokohama 235-8523

Since the accident of Fukushima Daiichi Nuclear Power Station caused by the Great East Japan Earthquake, higher safety has been required for the nuclear power plants. In order to prevent hydrogen explosion, we need to monitor the oxygen concentration in the PCV (Primary Containment Vessel) during the severe accident. We considered that the limiting current type oxygen sensor is promising as a measurement device that can be used under the condition inside the PCV at the severe accident. We evaluated the properties of a sensor using platinum or gold as the electrode material. We verified that the sensor with platinum electrode indicated low value when hydrogen and oxygen coexist. On the other hand, this phenomenon did not occur for the sensor with gold electrode. From this result, we verified the possibility of measuring the oxygen concentration in the environment of PCV where hydrogen and oxygen coexist by setting the limiting current type oxygen sensor with gold electrode.

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33.

MEMSセンサを活用したガス置換状態確認手法の開発(第2報)

東京ガス

○五味保城、金澤一弘

DEVELOPMENT OF A METHOD TO CONFIRM THE TRANSITION OF
FLAMMABLE GAS FROM AIR BY THE USE OF A MEMS SEMICONDUCTOR GAS
SENSOR:2nd report

Yasushiro GOMI and Kazuhiro KANAZAWA

Energy System Research Institute, Tokyo Gas Co., Ltd.
Tsurumi-ku, Yokohama 230-0045

We have developed a method to confirm the transition of flammable gas from air by the use of a MEMS (Micro Electro Mechanical Systems) semiconductor gas sensor. When gas meters and pipes used for flammable gas, such as city gas or LPG, are set up or changed, internal air must be substituted to flammable gas. Using a conventional pumping-type gas detectors, air was checked to be replaced by flammable gas, however, the pumping-type gas detector took time to introduce sampling gas to the sensor and to emit that gas. By installing the MEMS semiconductor gas sensor in a pipe, we can catch the transition of the flammable gas more quickly, easily and safety than a conventional method. We report on the result of investigating the sensor response over time in the real field by fabricating a prototype with built - in MEMS sensor.

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34.

シリコンマイクロリング共振器水素ガスセンサの応答特性の検討

横国大

○山作直貴、松浦壮佑、西島喜明、荒川太郎、岡崎慎司

RESPONSE CHARACTERISTICS OF SILICON MICRORING RESONATOR
HYDROGEN GAS SENSOR

Naoki YAMASAKU, Sosuke MATSUURA, Yoshiaki NISHIJIMA, Taro ARAKAWA,
and Shinji OKAZAKI

Graduate School of Engineering, Yokohama National University, Faculty of Engineering,
79-5 Tokiwadai, Hodogaya-ku, Yokohama-city 240-8501, Japan

A novel optical sensor using platinum-loaded tungsten oxide (Pt/WO3) and silicon microring-resonator (MRR) for detection of hydrogen gas at room temperature was proposed and tested. The sensing principle is mainly based on the resonant wavelength shift caused by the catalytic combustion reaction of hydrogen. In the previous study, it was found that Pt/WO3 film could not be deposited uniformly on the surface of the MRR. In order to solve this problem, acetylene glycol surfactant was used and mixed with Pt/WO3 precursor solution. Field emission electron probe micro analyzer (FE-EPMA) images of the device surface showed that the Pt/WO3 layer successfully covered the target part of the MRR. A 580 pm resonant wavelength shift was observed with exposure to hydrogen gas. The sensitivity of the developed device was about 4 times larger than that of the previous one.

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35.

アンモニア検知極における窒化ホウ素の
ミリング処理の効果

愛媛大

○板垣吉晃、川渕貴史、猿丸英理、青野宏通、八尋秀典

EFFECT OF BEAD-MILLING OF BORON NITRIDE FOR AMMONIA SENSING ELECTRODE

Yoshiteru ITAGAKI, Takafumi KAWABUCHI, Eri SARUMARU, Hiromichi AONO, Hidenori YAHIRO

Graduate School of Science and Engineering, Ehime University,
Matsuyama-Shi, Ehime 790-8577

Human breath from lever and renal disease patients are known to increase to 15 ppm or higher. Therefore, exhaled NH3 can be a biomarker in a non-invasive breath test for such diseases. We reported that the Pt sensing electrode covered with the bead-milled hexagonal boron nitride (h-BN) increased the EMF response. However, the smaller size (0.15 m) of h-BN than the bead-milled ones was not effective for EMF enhancement. The FT-IR observation of the bead-milled h-BN suggested the formation of B-OH group in the h-BN crystal by the mechanical cleavage of the B-N bond. We concluded that the formed B-OH groups acted as a Brønsted acid site for the ammonia adsorption.

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36.

ゼオライト系レセプタを用いた固体電解質インピーダンス型
NOxセンサの応答特性

九工大

○福島悠太、高瀬聡子、清水陽一

NOx SENSING PROPERTIES OF SOLID ELECTROLYTE - BASED
IMPEDANCEMETRIC SENSOR WITH ZEOLITE RECEPTOR

Yuta FUKUSHIMA, Satoko TAKASE and Youichi SHIMIZU

Department of Applied Chemistry, Kyushu Institute of Technology,
1-1 Sensui-cho, Tobata, City of Kitakyushu, Fukuoka 804-8550

As nitrogen oxides (NOx) are one of the causatives of air pollution as well as markers of medical diagnosis, needs of high performance NOx sensors are increasing for these gas monitoring. Zeolites are known as porous materials having a good molecular sieve function, but it is difficult to apply electrode materials for gas sensors due to their high electric resistance. Then, we applied new sensor structure with solid electrolyte and zeolite as a transducer and receptor, respectively, in which the high resistances of zeolites are not significantly influence to the sensor device. In this study, the effects of the kinds of cations in zeolite on the NOx response properties were also investigated. It was found that the impedance response of the potassium doped Y-type zeolite / Na5DySi4O12 device showed high sensitivity and sensitivity to NO at 400ºC, 50 Hz.

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