Vol. 33, Supplement A (2017)

Proceedings of the 61th Chemical Sensor Symposium
Hachioji Tokyo, March 25-27, 2017

 

Abstracts



1.

WO3系半導体ガスセンサによるメチルメルカプタンの高感度検知

a長崎大院工、b長崎大工、cフィガロ技研株式会社

○上田太郎a、黄 振東a、樋口琴乃b、井澤邦之c、 鎌田 海a、兵頭健生a、清水康博a

WO3-BASED SEMICONDUCTOR GAS SENSORS
FOR THE SENSITIVE DETECTION OF METHYLMERCAPTAN

Taro UEDAa, Zhendong HUANGa, Kotono HIGUCHIb,
Kuniyuki IZAWAc, Kai KAMADAa, Takeo HYODOa and Yasuhiro SHIMIZUa

aGraduate School of Engineering, bSchool of Engineering, Nagasaki University,
1-14 Bunkyo-machi, Nagasaki 852-8521
cFigaro Engineering Inc.,1-5-11 Senbanishi, Minoo, Osaka 562-8505

WO3-based semiconductor gas sensors were fabricated, and their sensing properties to methylmercaptan (CH3SH) were examined in this study. The Ru loading on WO3 increased the magnitude of CH3SH response, and the response increased with a decrease in the operating temperature as well as with an increase in thickness of the Ru-loaded WO3 films. In addition, the increase in porosity of the Ru-loaded WO3 films, which were fabricated by utilizing polymethylmethacrylate microspheres as a template, was effective in improving the CH3SH response, especially at a lower temperature of 150°C. The catalytic activities of CH3SH oxidation over WO3-based powders in wet air showed that CH3SH was partially decomposed to produce an intermediate (dimethyl disulfide) at temperatures less than 330°C and the Ru loading on the WO3 powder increased the catalytic activities. These results indicate that the positively charged adsorption of the intermediate onto the WO3-based powders is one of important factors in promoting the magnitude of CH3SH response at lower operating temperatures.

To Japanese Contents / To English Contents


2.

紫外線照射下での酸化物半導体ガスセンサの応答特性

長崎大院工a、長崎大工b

○兵頭健生a、岩永  梓b、浦田  薫a、石田圭司郎a 鎌田  海a、上田太郎a、清水康博a

GAS-SENSING PROPERTIES OF OXIDE SEMICONDUCTOR SENSORS
OPERATED UNDER UV-LIGHT IRRADIATION

Takeo HYODOa, Azusa IWANAGAb, Kaoru URATAa, Keijiro ISHIDAa, Kai KAMADAa,
Taro UEDAa, and Yasuhiro SHIMIZUa

aGraduate School of Engineering, bSchool of Engineering, Nagasaki University 1-14 Bunkyo-machi, Nagasaki 852-8521

Effects of UV-light irradiation (peak wavelength: 365 nm) on sensing properties of oxide semiconductor sensors to different gases (hydrogen, ethanol, NO2) have been investigated at 30°C in dry air. The responses of both pristine SnO2 and 0.05 wt% Pd-loaded SnO2 sensors to 6000 ppm H2 decreased and those to 50 ppm ethanol increased with an increase in UV-light irradiation intensity. In addition, the Pd loading onto the SnO2 surface was effective in improving the H2 response under weak UV-light irradiation as well as the ethanol response under strong UV-light irradiation. On the other hand, UV-light irradiation reduced the response of a porous (pr-)In2O3 sensor to 5 ppm NO2 and accelerated the response and recovery speeds at 30°C, and the 0.5 wt% Au loading onto the pr-In2O3 surface improved the NO2 response, especially under weak UV-light irradiation. However, the NO2 responses of pr-In2O3 sensors loaded with and without 0.5 wt% Au under UV-light irradiation at 30°C are not larger than those at elevated temperatures (no UV-light irradiation).

To Japanese Contents / To English Contents


3.

La2O3/SnO2積層素子のCO2センシング特性 に対する素子形状の影響

豊橋技術科学大学

○松田恭輔、岩田達哉、高橋一浩、澤田和明

INFLUENCES OF DEVICE GEOMETRY ON CO2 SENSING CHARACTERISTICS OF
La2O3/SnO2 STACKED DEVICE

Kyosuke MATSUDA, Tatsuya IWATA, Kazuhiro TAKAHASHI and Kazuaki SAWADA

Toyohashi University of Technology, Toyohashi-shi, Aichi 441-8122

In this study, fundamental characteristics of semiconductor CO2 sensors were investigated for integrated CO2 sensors. As sensing materials, La2O3 and SnO2 were employed. For the objective, La2O3SnO2 stacked structures device having simple two facing electrodes with 500-μm spacing: #1 and the device having comb electrodes with 20-μm spacing: #2, were fabricated. In addition, SnO2 with the various thicknesses in the range of 16 - 107 nm was deposited in #1. The resistance at 300°C in the synthesized air (N2: 80% + O2: 20%), Rair and that in the atmosphere with CO2, RCO2 were measured. The sensitivity was defined as the ratio of these resistances: Rair/RCO2. The sensitivity for 1000 ppm of CO2 of #1 increased as the SnO2 thickness decreased. The sensitivity of #2 with 107 nm of SnO2 thickness to 1000 ppm of CO2 was higher than one of #1. These sensitivity differences were investigated in terms of the CO2 concentration dependence and the electrode geometry based on the CO sensing model. As a result, it was suggested that the resistance near the interface with the electrodes contributes to the CO2 responses.

To Japanese Contents / To English Contents


4.

La2O3/SnO2積層素子を用いたMEMS CO2センサの検出特性

豊橋技術科学大学

○岩田達哉、Carine Soo Wei Ping、松田恭輔、高橋一浩、澤田和明

SENSING CHARACTERISTICS OF MEMS CO2 SENSORS BASED ON La2O3/SnO2
STACKED DEVICE

Tatsuya IWATA, Wei Ping Carine SOO, Kyosuke MATSUDA, Kazuhiro TAKAHASHI,
and Kazuaki SAWADA

Toyohashi University of Technology, Toyohashi-shi, Aichi 441-8122

In this study, we first report semiconductor CO2 sensors integrated with MEMS hotplates (MHP). The MHP has bridge-type structure with the membrane area of 140 μm × 140 μm. The membrane consists of SiO2 and SiN, and polycrystalline Si was used as a heater material. The MHP can reach 550°C at 5 V. The sensor consists of La2O3/SnO2 stacked material with Pt interdigitated electrodes. The CO2 sensitivity of the sensor for 1000 ppm CO2 increased with increasing the operating temperature, reaching the maximum of 2.9 at 350°C, while the sensitivity decreased with further increasing temperature. Similar temperature dependence was observed in Ra. Because the change in Ra may originate from the density and ionization state of adsorbed oxygen, the results indicate that the adsorbed oxygen plays an important role in CO2 sensing by the sensor in this study. Furthermore, it was confirmed that the limit of detection was 200 ppm at 400°C, at which the power consumption of the heater was about 20 mW. Consequently, a low-power MHP-based CO2 sensor was demonstrated.

To Japanese Contents / To English Contents


特別講演

アセトンを中心とした ドコモの生体ガスセンシングへの取り組み

NTTドコモ先進研

○檜山 聡

DOCOMO’S INITIATIVE ON BIOLOGICAL GAS SENSING
TARGETED FOR ACETONE

Satoshi HIYAMA

Research Laboratories, NTT DOCOMO, Inc.
Yokosuka-shi, Kanagawa 239-8536

NTT DOCOMO is researching and developing self-health management using biological data in the health and medical care fields. Biological data reflecting individual differences can be obtained from biological gas contained in human breath and emitted from the skin’s surface without the pain of drawing blood, and that data can be applied to self-health management. We have developed three types of devices that measure biological gas components contained in breath or emitted from the skin’s surface for use as markers of fat metabolism, alcohol intoxication, and other conditions. These easy-to-use devices are expected to be useful in alleviating and preventing obesity, which can lead to all sorts of diseases and raise the risk of acquiring lifestyle-related diseases.

To Japanese Contents / To English Contents


5.

アルカリ土類フェライトのCO2 ガス検知特性における異種元素の添加効果

北九州高専

○小畑賢次、水田圭祐、松嶋茂憲

EFFECT OF ADDITIONAL ELEMENT ON CO2 SENSING PROPERTIES
OF ALKALI EARTH FERRITE

Kenji OBATA, Keisuke MIZUTA, Shigenori MATSUSHIMA

Department of Creative Engineering, National Institute of Technology (NIT),
Kitakyushu College, Shii 5-20-1, Kokuraminami-ku, Kitakyushu 802-0985

CO2 sensing properties of alkali earth ferrite (MgFe2O4 and CaFe2O4) added with additional element, such as Si, Ti, Hf, Zr, La and Sm, were examined in the temperature range of 250 to 500 °C in dry air. At 300 °C, CO2 sensitivity of CaFe2O4 was effectively improved by adding a small amount of impurity atoms such as Zr and Hf in comparison with unadded one. On the other hand, CO2 sensitivities of MgFe2O4-based sensors were lower than those of CaFe2O4-based one. CO2 sensitivity of the Zr-added CaFe2O4 sensor reached maximum at measuring temperature between 300 and 350 °C, and was estimated to be 2.9 times higher than that of pure CaFe2O4 powder. At 350 °C, for 5 mol% Zr-added CaFe2O4, the CO2 sensitivity was the highest among the sensors examined.

To Japanese Contents / To English Contents


6.

プロトン導電体を用いて酸素分圧を制御した半導体NOxセンサの検知特性

名大院環境a、日本自動車部品総合研究所b

○長尾征洋a、小林和代a、呂 佩玲a、寺西真哉b、日比野高士a

SENSING PERFORMANCES OF SEMICONDUCTOR NOX SENSORS USING PROTON
CONDUCTOR FOR OXYGEN PARTIAL PRESSURE CONTROL

Masahiro NAGAOa, Kazuyo KOBAYASHIa, Peiling Lva, Shinya Teranishib
and Takashi HIBINOa

aGraduate School of Environmental Studies, Nagoya University,
Nagoya-shi, Aichi 464-8601
b R&D dev1, Nippon Soken, Inc., Nishio-shi, Aichi 445-0012

Highly oxidative and reductive state of WO3 and In2O3 coupled with NOx detection was studied by attaching the metal oxide electrode on an Sn0.9In0.1P2O7 electrolyte and by polarizing the electrode at 200 °C. During the polarization, insertion and release of H+ occurred at the WO3 electrode and redox reaction of metal ions occurred at In2O3 electrode. Hydrogen tungsten bronze was not sensitive to NOx, however, WO3 showed a comparable sensitivity toward NOx. The ohmic, charge-transfer, and gas-diffusion resistances changed by the exposition to NOx. The changes in NOx sensing property of In2O3 was observed when the polarity and applied voltage were changed. During cathodic polarization in the range of -0.5 to -1.5 V, the resistance of the sensor decreased when NOx were supplied to the sensor. On the other hand, the resistance increased by the supply, especially of NO2 at -2.0 V. This phenomena attributed to the reduction of ions and functioned as selective adsorption sites especially for NO2. Both electrodes were little affected by interference gases.

To Japanese Contents / To English Contents


7.

交流インピーダンス法による酸化セリウム系酸素センサの解析

産総研a、ミナト医科学b

○伊藤敏雄a、伊豆典哉a、申 ウソクa、上堀内健二b、滝澤正夫b、吉田 功b

INVESTIGATION OF CERIUM OXIDE-BASED OXYGEN SENSORS BY
ALTERNATING CURRENT IMPEDANCE METHOD

Toshio ITOHa, Noriya IZUa, Woosuck SHINa, Kenji KAMIHORIUCHIb,
Masao TAKIZAWAb, and Isao YOSHIDAb

aNational Institute of Advanced Industrial Science and Technology (AIST), Shimo-shidami, Moriyama-ku, Nagoya 463-8560
b Minato Medical Science Co., Ltd. Minatojima, Chuo-ku, Kobe 650-0047

Cerium oxide is one of the most promising resistive oxygen sensing materials for use in fast-response sensors because the cerium oxide possesses high diffusion coefficient for oxygen so that oxygen vacancies can diffuse to whole bulk of cerium oxide and clear electrical resistance changes are obtained. Though pure cerium oxide possesses high impedance, the resistance of 10% Zr-doped cerium oxide (Ce0.9Zr0.1O2; CeZr10) is roughly one tenth of that of pure cerium oxide with almost the same performance for oxygen sensor. In this study, we investigate sensing properties of CeZr10 to simulated exhaled gas and evaluation of CeZr10 using alternating current impedance analysis for developing as breath oxygen sensors.

To Japanese Contents / To English Contents


8.

水素検知における酸化タングステンナノ結晶への酸素欠陥導入効果

熊本大院 a、熊本大パルスb

◯松本和也a、橋新 剛a,b、三上一輝a、木田徹也a、 松田 元秀a、久保田 弘b

EFFECT OF OXYGEN VACANCY DOPING ON TUNGSTEN OXIDE
NANOCRYSTALS IN HYDROGEN DETECTION

Kazuya MATSUMOTOa, Takeshi HASHISHINa,b, Kazuki MIKAMIa,
Tetsuya KIDAa, Motohide MATSUDAa, and Hiroshi KUBOTAb

aGraduate School of Science and Technology, Kumamoto University,
2-39-1, Kurokami, Kumamoto 860-8555
bInstitute of Pulsed Power Science, Kumamoto University,
2-39-1, Kurokami, Kumamoto 860-8555

Sensing properties of WO3 with different amount of oxygen vacancy to hydrogen were examined using Au counter electrodes. WO3-O2 with low amount of oxygen vacancy showed n-type semiconductor behavior. On the other hand, WO3-H2 with many amount of oxygen vacancy showed p-type semiconductor behavior. This behavior suggests that hole is formed due to many amount of oxygen vacancy. The behavior of WO3-Air was also depending on operating temperature. The WO3-Air behaved as n-type behavior at the operating temperature of 300 and 250 oC as well as p-type behavior at the temperature of 200 oC. From above results, oxygen vacancies doped by three types of hydrothermal atmosphere were related to hydrogen detection.

To Japanese Contents / To English Contents


9.

カーボンナノホーンを用いたガスセンサ

NEC IoTデバイス研究所

○殿内規之、弓削亮太、遠藤浩幸、二瓶史行

GAS SENSORS USING CARBON NANOHORNS AS GAS SENSITIVE MATERIALS

Noriyuki TONOUCHI , Ryota YUGE, Hiroyuki ENDOH, Fumiyuki NIHEY

IoT Devices Laboratories, NEC Corporation
Tsukuba, Ibaraki 305-8501

Carbon nanohorns (CNHs) exhibit a high-surface-area architecture and have a large number of reactive carbon edge sites, which make them a promising candidate for a molecular adsorber. We have recently discovered a new nano-carbon material, "carbon nanobrush," (CNB) a fibrous aggregate composed of radially-assembled graphene-based single-walled nanotubules. CNB also has high surface area to volume ratios but has more than 10 times the electrical conductivity of CNHs. Due to these remarkable properties, they are expected to be a promising material for gas sensitive resistors. In this report, we examined the applicability of CNB for gas sensitive films by measuring the response to VOC gas exposures at the concentration between 200ppm and 4000ppm. We observed apparent resistive changes according to the VOC gas exposure, dependency on the gas concentrations, and the reversibility of the response. As a result, we confirmed applicability of CNB for the gas sensitive films.

To Japanese Contents / To English Contents


10.

ガス直焚吸収冷温水機用COモニターの開発

エフアイエス株式会社a、日立ジョンソンコントロールズ空調株式会社b、新光商事株式会社c

○黒江 靖a、松本晋一a、土井茂裕b、岩澤文典c

DEVELOPMENT OF CO MONITOR FOR GAS DIRECT FIRED ABSORPTION
CHILLER-HEATERS

Yasushi KUROEa, Shinichi MATSUMOTOa, Shigehiro DOIb and Fuminori IWASAWAc

a FIS Inc., 3-36-3 Kitazono, Itami, Hyogo 664-0891
bHitachi - Johnson Controls Air Conditioning Inc.,
603 Kandatsu-machi Tsuchiura, Ibaraki 300-0013
c Shinko Shoji Co.,Ltd., Bungei Blog. 1-4-5 Toranomon Minatoku, Tokyo 105-0001

Gas direct fired absorption chiller-heaters are used for the air conditioning of large buildings and factories. At present we carry out periodic safety inspections by a technical maintenance person, that is why there is a demand for an inexpensive monitor that can detect CO gas at any time. For this development we used an existing semiconductor CO gas sensor to which we added water- and heat- resistant structure to install it in a chimney flue. This sensor element alternates high temperature and low temperature cycle to detect CO. The drive was tested over a long period it showed stability and was able to detect CO. By utilizing the change ratio of this sensor resistance from the resistance in normal combustion as a relative response, this module can detect CO without any influence from other gases except CO gas. We found that the unburned flammable gas is transiently released at the time of ignition. Since this gas could cause a false alarm, we decided to use a way to turn off gas detection temporarily. We succeeded in developing a CO monitor for gas absorption chiller-heaters.

To Japanese Contents / To English Contents


11.

蓄積型NO検出素子の反応機構解析

東北工大工a、東北工大院工b

○浅沼光吾a、日野慎司b、鈴木義史a、丸尾容子a,b

CHEMICAL KINETIC REACTION MECHANISM FOR NO DETECTION
IN AN ACCUMULATE TYPE SENSOR ELEMENT

Kohgo ASANUMAa, Shinji HINOb, Yoshifumi SUZUKIa and Yasuko Y. MARUOa,b

aFaculty of Engineering, Tohoku Institute of Technology,
Sendai-shi, Miyagi 982-8577
bGraduate School of Engineering, Tohoku Institute of Technology,
Sendai-shi, Miyagi 982-8577

Nitrogen monoxide (NO) is an important bio-regulatory molecule, and high concentrations of NO are observed in the air exhaled by patients with asthma. Therefore we considered that a method to detect NO with both high sensitivity and high selectivity is required. We have already developed the NO sensing element using 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxil(PTIO) impregnated in a porous glass with 4 nm pore size, and have examined both the performance of the sensing element at 0.625 ppm NO atmosphere and the interference gases. In this work, we evaluated the kinetic reaction constant between the reagent and gaseous NO and obtained values for the solution system and the sensing element were 6.73×10-3 Lmol-1min-1 and 1.21×10-3 Lmol-1min-1, respectively. Despite the passive air sampling system of the proposed sensing element, the reaction constant of the sensing element was one-six of liquid’s one that was obtained in an active bubbling system. In addition, we also evaluated the sensing element’s performance by exposing to 0.167ppm NO. As a result, good linearity was observed between accumulated NO concentrations and the absorbance changes, that indicating the possibility of the detection with ppb.

To Japanese Contents / To English Contents


12.

Pt/SiO2感応膜を用いた
光ファイバーグレーティング型水素センサの高感度化

横浜国立大学

○一里山誠治、岡崎慎司、笠井直哉、黒肱麻理奈

SENSITIVITY IMPROVEMENT OF FIBER-BRAGG-GRATING TYPE
HYDROGEN SENSOR USING Pt/SiO2 CATALYST FILM

Seiji ICHIRIYAMA, Shinji OKAZAKI, Naoya KASAI, and Marina KUROHIJI

Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku,
Yokohama-city, Kanagawa 240-8501

Hydrogen gas sensor for leak detection is indispensable for wide spread use of hydrogen energy system. The authors propose catalyst-type hydrogen gas sensor using fiber Bragg gratings (FBG). Sensing principle is based on catalytic combustion heat and related thermal strain which makes shifts of center wavelength of the reflection spectra. In this study, Pt/SiO2 catalyst film as a hydrogen-sensitive layer was obtained by using sol-gel method. The precursor solution was composed of hexachloroplatinic acid and commercially available silica precursor solutions. The molar ratio between the Si and Pt was fixed at 13:1. Small amount of this solution was dropped on the substrate and dried at room temperature. After that, the film was calcined at 500 ℃ in air. In order to enhance the sensitivity, this procedures were repeated and therefore thick hydrogen-sensitive films were obtained. It is found that sensor device having thick Pt/SiO2 film showed high sensitivity at room temperature even in the low concentration range.

To Japanese Contents / To English Contents


13.

低温作動可能な新規な接触燃焼式水素ガスセンサ

阪大院工

田村真治、安原 駿、今中信人

A NEW CATALYTIC COMBUSTION TYPE HYDROGEN
GAS SENSOR OPERATING AT LOW TEMPERATURE

Shinji TAMURA, Shun YASUHARA and Nobuhito IMANAKA

Department of Applied Chemistry, Faculty of Engineering, Osaka University,
2-1 Yamadaoka, Suita, Osaka

We have developed the catalytic combustion type hydrogen gas sensor showing high sensing performance even at moderate temperatures by incorporating a Pt loaded CeO2–ZrO2–SnO2 solid solution as the H2 gas oxidation catalyst. Since the present catalyst can oxidize H2 gas completely even at 55°C, the sensor with a Pt loaded CeO2–ZrO2–SnO2 solid solution showed superior H2 gas sensing performance at the temperature as low as 55°C, which is substantially lower than that required for the operation of the sensors with the conventional H2 oxidation catalysts such as Pt / Al2O3 or Pd / Al2O3.

To Japanese Contents / To English Contents


14.

固体電解膜式COセンサ検知機構の電気化学的解析

大阪ガス株式会社a、フィガロ技研株式会社b

○野中 篤a、大西久男a、岡田正文b、藤森裕樹b

ELECTROCHEMICHAL ANALYSIS OF SENSING MECHANISM
OF A SOLID ELECTROLYTE MEMBRANE TYPE CO SENSOR

Atsushi NONAKAa, Hisao OHNISHIa, Masafumi OKADAb and Yuki FUJIMORIb

aOsaka Gas Co., Ltd., Torishima, Konohana-ku, Osaka 554-0051
bFigaro Engineering Inc., Senbanishi, Mino-shi, Osaka 562-8505

In order to clarify the electrochemical behavior at detecting CO, polarization curves of a working electrode and a counter electrode of a solid electrolyte membrane type CO sensor were measured using Pt wire as a reference electrode. As with a liquid electrolyte membrane type sensor, short-circuit current between both electrodes in the air containing CO was confirmed to be approximately a half of diffusion limited current of a CO oxidation reaction on the working electrode.

To Japanese Contents / To English Contents


15.

酸素ポンプ電流を利用する電流検出型アンモニアガスセンサ

九州大学大学院工学研究院a、九州大学カーボンニュートラルエネルギー国際研究所(WPI-I2CNERb

○石原達己a,b、藤原岳史a、深町桂子a、伊田進太郎a,b

AMPEROMETRIC NH3 SENSOR BASED ON OXYGEN PUMPING CURRENT

Tatsumi ISHIHARAa,b, Takeshi FUJIWARAa, Keiko FUKAMACHIa, Shintaro IDAa,b

aDepartment of Applied Chemistry, Faculty of Engineering, Kyushu University,
Nishi-ku, Fukuoka 819-0395
bInternational institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyusyu University,
744 Motooka, Nishi-ku, Fukuoka 819-0395

Ammonia ( NH3 ) is extremely toxic and harmful for environmental. Recently, NH3 from urea is used for selective catalytic reduction (SCR) of NOx from diesel engines. Since little excess amount of NH3 is required for high NOx conversion, leakage of ammonia is anticipated for the current urea NOx SCR system. Therefore, detection of NH3 in exhaust gas line from diesel engines is an important subject. Various types sensor have been proposed for NH3 detection. In particular, semiconductor type sensor or galvanic battery type sensor are popularly used. However, because of selectivity, maintenance and operating temperature, development of NH3 sensor with more sensitive and selective is strongly required. In this study, we investigated NH3 sensor based on oxygen pumping current and it was found that the cell using LaFeO3 for NH3 oxidation, LaGaO3 doped with Ni for electrolyte, Au-In0.95Sn0.05O1.5 (ITO) inactive electrode shows high sensitivity to NH3 in air.

To Japanese Contents / To English Contents


16.

窒化ホウ素で電極被覆した起電力式センサの
アンモニア検知特性

愛媛大院理工

○板垣吉晃、川渕貴史、猿丸英理、青野宏通、八尋秀典

DETECTION OF AMMONIA BY POTENTIOMETRIC SENSORS WITH BORON
NITRIDE COVERED ELECTRODES

Yoshiteru ITAGAKI, Takafumi KAWABUCHI, Eri SARUMARU, Hiromichi AONO and Hidenori YAHIRO

Graduate School of Science and Engineering, Ehime University,
Matsuyama-Shi, Ehime 790-8577

Human breath from a controlled human body contains <1 ppm NH3, while that from a lever disease patient is known to increase to 50 ppm. Therefore NH3 can be a biomarker in a non-invasive breath test for lever disease. In this study, potentiometric sensors aiming at detecting NH3 in human breath were examined. Zirconia based sensor elements, i.e., NH3, Pt/YSZ/Pt, air, detected an air-balanced 30ppm NH3. Covering the Pt sensing electrode with hexagonal boron nitride (h-BN) supported catalysts (Pt, Pd, Ag) increased the EMF response. In particular, Ag loaded h-BN (Ag/h-BN) was the most effective catalyst, and the EMF response increased with an increase in the amount of Ag loading between 2 and10wt%. In the case of 10wt%Ag/h-BN, the EMF response value for 30ppm NH3 was 60 mV and 90% response time was 220 s. However, Ag/h-BN was not significantly active for NH3 decomposition. Therefore, it was deduced that the loaded Ag rather acts as NH3 adsorption sites to condense NH3 molecules in the catalyst layer. Humidification of NH3 significantly reduced the EMF response compared to the dry NH3 case.

To Japanese Contents / To English Contents


17.

層状ペロブスカイト型酸化物厚膜を用いた
インピーダンス検知型COセンサ

九工大院工

山元信佑、高瀬聡子、○清水陽一

IMPEDANCEMETRIC THICK-FILM CO SENSOR USING LAYERED
PEROVSKITE-TYPE OXIDE

Shinyu YAMAMOTO, Satoko TAKASE, and Youichi SHIMIZU

Department of Applied Chemistry, Kyushu Institute of Technology,
Tobata, City of Kitakyushu, Fukuoka 884-8550

Carbon monoxide (CO) is one of the most toxic gases in air pollution and human life. In previous researches, Cu-based materials have been used for CO sensors. In this study, an impedancemetric CO sensor using layered perovskite-type cuprates has been investigated. Layered perovskite-type La2-xA’xCu1-yB’yO4 (A’ = Sr, Ba, Ce, B’ = Zr, x, y =0.1, 0.2) cuprate powders, which were used as a receptor material for CO sensor, were synthesized by a polymer precursor method. Thick-film devices prepared by screen-printing method were investigated by an AC impedance method at 300-500ºC. Among the Cu-based oxides tested, La1.9Ce0.1CuO4 device showed good CO response which increasing with increasing CO concentrations at 400ºC, 50 Hz. According to XPS analysis, it could realize that the La1.9Ce0.1CuO4 has a larger amount of Cu+ specie than the other oxides. It should consider that the Cu+ plays an important role in CO sensing.

To Japanese Contents / To English Contents


18.

フェロセン修飾DNAアプタマーおよび制限酵素エクソヌクレアーゼIII
を組み合わせた検出系の設計

パナソニック(株)a、兵庫県立大院物質理b

○有本 聡a、田中真司a、下野 健a、安川智之b、水谷文雄b、吉岡俊彦a

DESIGN OF DETECTION SYSTEM USING FERROCENE
CONJUGATED DNA APTAMER AND EXONUCLEASE III

Satoshi Arimotoa, Shinji Tanakaa, Ken Shimonoa, Tomoyuki Yasukawab,
Fumio Mizutanib, Toshihiko Yoshioka a

aAdvanced Research Division Device Research Laboratry, Bio Research Department,
Panasonic Corporation, 3-4 Hikaridai, Seika, Soraku, Kyoto 619-0237, Japan
bGraduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori,
Ako, Hyogo 678-1297

It is well known that cocaine binding aptamer changes its conformation when it captures cocaine as a target molecule. Usually, cocaine aptamer is immobilized on gold electrode using gold-sulfur interaction to prepare cocaine sensor, which accompanies undesired aggregation and non-specific adsorption. We have already reported that these problems could be improved by simple heat treatment after immobilization. However, in systems that immobilize aptamers, since only one electron signal derived ferrocene molecule could be obtained from one aptamer that captures cocaine, it was difficult to amplify the signal and improve sensitivity. In this presentation, combining a ferrocene-modified cocaine-binding aptamer with a restriction enzyme exonuclease III that specifically degrades double-stranded DNA to release ferrocene molecule into the solution, we designed a new system in which ferrocene was amplified with an interdigitated array electrodes. The sensitivity of cocaine was improved compared with the immobilization systems.

To Japanese Contents / To English Contents


19.

ポリマー圧電素子を用いた
DNA検出用バイオセンサの検知特性

東京都市大学a、北海道大学人獣共通感染症リサーチセンタb

○武田真理子a、矢萩洸貴a、杉山龍男a、平田孝道a、中島千絵b、鈴木定彦b、 宗像文男a

SENSING PROPERTIES OF BIOSENSOR WITH PIEZOELECTRIC POLYMER
FOR DNA DETECTION

Mariko Takedaa, Kouki Yahagia, Tatsuo Sugiyamaa, Takamichi Hirataa,
Chie Nakajimab, Yasuhiko Suzukib and Fumio Munakatasupa

aTokyo City University,
Setagaya-ku, Tokyo 158-8557
bResearch Center for Zoonosis Control, Hokkaido University,
Kita-ku, Sapporo 001-0020

The conventional method for the diagnosis of infective diseases (the loop-mediated isothermal amplification (LAMP)) is limited for the detection time. Therefore, a biosensor using a quartz crystal is attracted for such infection diagnoses. The sensor with the quartz crystal is monitoring mass changes of targets in high frequency. However, the properties of polymer materials (DNA etc.) do not response to the vibration in the range of high frequency depend on molecular size. In this report, we are developing a new sensor using a piezoelectric polymer so called PolyVinylidene DiFluoride (PVDF). Because PVDF responses to a wide range of frequencies, the measuring at relaxation frequency is appropriate to detect micro mass changes of polymer (DNA etc.).

To Japanese Contents / To English Contents


20.

フッ素樹脂を含有する新規電解質インクを用いた
スクリーン印刷型参照電極の作製と評価

東理大理工a、東理大総研院b

○古茂田将人a、星芳直a、四反田功a,b、板垣昌幸a,b

FABRICATION AND CHARACTERIZATION OF SCREEN-PRINTED REFERENCE ELECTRODE
USING FLUORINE-BASED RESIN

Masato Komoda,a Yoshinao Hoshia,Isao Shitandaa,b and Masayuki Ttagakia,b

aDepartment of Pure and Applied Chemistry, Faculty of Science,
Tokyo University of Science Noda, Chiba 278-8510, Japan
bResearch Institute of Science and Technology, Tokyo University of Science
Noda, Chiba 278-8510, Japan

In this research, we developed a new reference electrode using poly vinylidene di fluoride (PVdF) binder and micro silica gel for thin layer chromatography to compatibilize two printed reference electrodes we have developed, a reference electrode with PDMS harden electrolyte layer and liquid junction layer having long lifetime, and a paper-based reference electrode indicated the stable potential fast. Using these materials, the electrolyte layer inside the reference electrode formed strong hydrophilic electrolyte layer, and kept KCl, leads to stable potential. We characterized this screen-printed reference electrode using potentiometry and cyclic voltammetry. As the result of potentiometry, the screen-printed reference electrode shift stable in 3 minutes from start, and kept stable for 5000 minutes.

To Japanese Contents / To English Contents


21.

化学イメージセンサを用いたイオンの拡散係数評価

東北大工a、東北大医工b

○宮本浩一郎a、内藤祥子a、吉信達夫a,b

EVALUATION OF DIFFUSION COEFFICIENT OF IONS
USING CHEMICAL IMAGING SENSOR

Ko-ichiro Miyamotoa, Shoko Naitoa and Tatsuo Yoshinobua,b

aDepartment of Electronic Engineering, Tohoku Univ.
b Department of Biomedical Engineering , Tohoku Univ.
6-6-05 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan

In this study, we constructed a microfluidic channel on the chemical imaging sensor, which is capable of visualizing pH distribution. In our previous studies, the non-uniformity of signal photocurrent in the channel was a problem. In the present study, the ceiling of the channel was made of glass with conducting ITO to enhance the uniformity. Diffusion of ions across the boundary between two laminar flows was quantitatively measured in a Y-shaped channel and it was demonstrated that diffusion coefficients of ions with only a small difference in ionic radius could be distinguished.

To Japanese Contents / To English Contents


22.

転写印刷によるウェアラブル
ナトリウムイオンセンサの作製

東京理科大学a、東京理科大学総合研究院b

〇渡邊和紀a、古茂田将人a、星 芳直a、四反田 功a,b、板垣昌幸a,b

FABRICATION OF A WEARABLE SODIUM ION SENSOR
USING TRANSFER PRINTING TECHNIQUE

Kazuki WATANABEa,Masato KOMODAa,Yoshinao HOSHIa
Isao SHITANDAa,b,Masayuki ITAGAKIa,b

aDepartment of Pure and Applied Chemistry, Faculty of Science and Technology,
Tokyo University of Science, Noda-shi, Chiba 278-8510
bResearch Institute for Science and Technology, Tokyo University of Science,
Noda-shi, Chiba 278-8510

Electrochemical wearable devices have been attracted attention as a device for evaluating the health condition by analysis of perspiration. Sodium ion is an excellent marker for electrolyte imbalance. In this study, we newly prepared a wearable potentiometric sodium ion sensor by transfer printing technique. The sensor was formed on a PET film, on which a releasing agent was coated, by screen-printing. Firstly, prior to perform the transfer printing, the responses of the screen-printed sensor on the PET film to sodium ion were evaluated by measuring the open circuit potential in a NaCl solution (0.1 - 100 mM). As a result, the sensor responded linearly by obeying the Nernst equation under 0.1 - 100 mM range. The slope of the calibration curve was 52.3 mV log10[Na+]-1. In addition, we evaluated the performances of the wearable sodium ion sensor.

To Japanese Contents / To English Contents


23.

金属置換ク−ロメトリ−による
微量溶液中成分の高感度検出

筑波大院数理物質

○滝江秀一、土谷信之介、鈴木博章

HIGHLY SENSITIVE COULOMETRIC DETECTION OF AN ANALYTE
IN A SOLUTION OF A SMALL VOLUME COUPLED WITH METALLIZATION

Shuichi TAKIE, Shinnosuke TSUCHIYA and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8573

An electrochemical sensing device based on the oxidation of an analyte on an electrode in a flow channel and deposition of silver in the other flow channel and subsequent coulometric measurement of the deposited silver was developed. To improve the detection limit, the effect of decreasing the number of electrodes in a microelectrode array for the deposition of silver and reducing the electrode area were examined. In the former case, measured charges did not decrease as long as there were a sufficient number of microelectrodes. On the other hand, no change in measured charges was observed in the latter case. In both cases, the background charge decreased by decreasing the number and area of the microelectrodes. Furthermore, a device with flow channels connected with two Ag/AgCl electrodes was used. The charge could be amplified by changing the potential of the Ag/AgCl electrodes.

To Japanese Contents / To English Contents


24.

マルチ電解修飾カーボン電極を用いる溶存水素の電気化学センサの開発

埼玉工大工a、埼玉工大院工b

〇松浦宏昭a,b、坂本珠羅b、木村弘幸a、内山俊一b

DEVELOPMENT OF ELECTROCHEMICAL SENSOR FOR DISSOLVED HYDROGEN
MOLECULE USING MULTIELECTROLYTIC MODIFIED CARBON ELECTRODES

Hiroaki MATSUURAa,b, Shura SAKAMOTOb, Hiroyuki KIMURAa and Shunichi UCHIYAMAb

aDepartment of Life Science & Green Chemistry, Faculty of Engineering, Saitama Institute of Technology,
1690 Fusaiji, Fukaya, Saitama 369-0293
bDepartment of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology,
1690 Fusaiji, Fukaya, Saitama 369-0293

Flow injection analysis (FIA) with amperometric determination of dissolved hydrogen molecule (H2) was first developed using electrodeposited platinum particles on glassy carbon electrodes modified with nitrogen-containing functional groups (Pt-NMGC) as the working electrode. A glassy carbon (GC) electrode was covalently modified by electrochemical oxidation/reduction procedures. Intriguingly, the redox waves between hydrogen ion and H2 at highly positive potential range were obtained by using Pt-NMGC electrode. The specific electrocatalytic activity for the electrode oxidation of H2 has successfully been applied to the FIA of dissolved H2. The typical current vs. time curve was obtained by the repetitive measurement of dissolved H2, and the measurement of dissolved H2 was finished completely in a short time (~20 sec.). A linear relationship was obtained between the oxidation current of H2 and dissolved H2 concentration. This indicates that our proposed technique can be used for the determination of the dissolved H2 concentration.

To Japanese Contents / To English Contents


25.

電解析出法を用いたグルコースオキシターゼ固定電極の作製

徳島大院先端技術科学a、徳島大院理工b

○礒合俊輔a、丹波 萌a、日裏健太郎a、倉科 昌b、安澤幹人b

FABRICATION OF GLUCOSE OXIDASE-IMMOBILIZED ELECTRODE
USING ELECTRODEPOSITION METHOD

Shunsuke ISOAIa, Megumi NIWAa, Kentaro HIURAa,
Masashi KURASHINAb and Mikito YASUZAWAb

a,bDepartment of Applied Chemistry, Tokushima University,
2-1 Minamijosanjima, Tokushima 770-8506

Glucose oxidase (GOx)-immobilized electrodes were prepared by the combination of electrodeposition and photopolymerization. Methacryloyl choline chloride (NMA) were employed as photo-polymeriable methacrylates and were added in the enzyme-electrdeposition solution, in order to be entrapped in the enzyme film. In order to investigate the enzyme film formation behavior during the electrodeposition process, quartz crystal microbalance (QCM) technique was employed. QCM measurement results indicated that the amount of deposit increased continuously up to around 15 minutes, while the decrease of deposit was observed after that time. The amount of GOx immobilized on the electrode was also measured using Amplex Red Assay Kit and showed that the decrease of deposit was parallel with the decrease of GOx on the electrode. However, the amount of enzyme on the electrode started to increase again after electrodeposition time of 40 minutes. The glucose sensor sensitivity of the obtained electrode showed a good correlation with the amount of enzyme immobilized on the electrode.

To Japanese Contents / To English Contents


26.

ホウ素ドープダイヤモンド微粒子を用いた
フレキシブル導電材料の植物生体電位センサへの応用

(公財)神奈川科学技術アカデミー(KAST)光触媒グループa、KAST高度計測センターb
東京理科大学光触媒国際センターc、日本工業大学工d

○田子祥子a、落合 剛a,b,c、鈴木清太郎a,b、林 美緒a、藤嶋 昭a,c

FLEXIBLE BORON-DOPED DIAMOND (BDD) ELECTRODES FOR PLANT MONITORING

Shoko TAGOa, Tsuyoshi OCHIAIa,b,c, Seitaro SUZUKIa,b, Mio HAYASHIa, and Akira FUJISHIMAa,c

aPhotocatalyst Group, Kanagawa Academy of Science and Technology (KAST),
Takatsu-ku, Kawasaki-shi, Kanagawa 213-0012
bMaterials Characterization Center, KAST, Takatsu-ku, Kawasaki-shi, Kanagawa 213-0012
c Photocatalysis International Research Center, Tokyo University of Science,
Noda-shi, Chiba 278-8510
dDepartment of Computer and Information Engineering, Nippon Institute of Technology,
Miyashiro-machi, Saitama 345-8501

A sensitive plant monitoring system with flexible boron-doped diamond (BDD) electrodes prepared from BDD powder and resin (Nafion or Vylon-KE1830) was investigated. The properties of the electrodes were compared with those of small BDD plate-type electrodes. While flexible BDD electrodes have wide potential windows, their cyclic voltammograms are not identical to those of the BDD plate. Further, the potential gap between a pair of electrodes attached to the plants changes as the plants are stimulated artificially with a finger touch, suggesting that the bioelectric potentials in the plant also changed, manifesting as changes in the potential gap between the electrodes. The BDD electrodes were assessed for their response reproducibility to a finger stimulus for 30 days. The plant monitoring system worked well with flexible BDD electrodes. Furthermore, the electrodes were stable and as reliable as the BDD plate electrodes in this study. Thus, a flexible and inexpensive BDD electrode system was successfully fabricated for monitoring the bioelectric potential changes in plants.

To Japanese Contents / To English Contents


27.

フェニレンジアミン誘導体を用いたプロテアーゼ基質の
開発と電気化学的エンドトキシン検出への応用

東北大院環境a、東北大工b、東北大WPI-AIMRc

○井上(安田)久美a、 鎌田祐麿b、 孫 思祥a、 池川未歩a、 伊藤―佐々木 隆広c、 末永 智一a,b,c

DEVELOPMENT OF PROTEASE SUBSTRATE COMBINED WITH PHENYLENEDIAMINE
DERIVATUVES AND ITS APPLICATION TO ENDOTOXIN DETECTON

Kumi Y. INOUEa, Yuma KAMADAb, Sixiang SUNa, Miho IKEGAWAa,
Takahiro ITO-SASAKIc, and Tomokazu MATSUEa,b,c

aGraduate School of Environmental Studies, Tohoku University, 6-6-11 Aoba, Aramaki,
Aoba, Sendai 980-8579
bSchool of Engineering, Tohoku University, 6-6-11 Aoba, Aramaki, Aoba, Sendai 980-8579,
cAdvanced Institute of Materials Research (WPI-AIMR), Tohoku University,
2-1-1 Katahira, Aoba, Sendai 980-8579,Katahira, Aoba, Sendai 980-8579

Previously we developed Leu-Gly-Arg-p-aminophenol (LGR-pAP) as a novel sub-strate of Limulus amebocyte lysate (LAL) assay for a simple amperometric detection of endotoxin. In this study we developed another substrates combined with two types of phenylenediamine derivatives alternative to pAP, p-amino-N-methylaniline (MPD) and N,N-dimethyl-p-phenylenediamine (DMPD). Both MPD and DMPD showed better temporal stability than pAP. In the voltammetric analysis, an oxidation peak was shown at 0.5 V vs Ag/AgCl in the case of 1mM LGR-pAP, LGR-MPD and LGR-DMPD in 0.1 M HEPES buffer (pH 7.8), while 0.2 V vs Ag/AgCl in the case of 1mM pAP, MPD and DMPD in the same buffer. From this result, we demonstrated endotoxin detection with amperometry at 0.3 V vs Ag/AgCl. A mixture of LAL reagent containing 1 mM substrate and endotoxin sample solution was incubated at 37°C for 1 h. Then, amperometry was conducted using glassy carbon disc electrode (1.0 mm in diameter) as a working electrode. By using this process, 10 endotoxin units L−1 were detected.

To Japanese Contents / To English Contents


28.

誘電泳動を用いたナノ炭素材料配向型電極デバイスの作製

東北大院工a、東北大院環境b

○珠玖 仁a、内藤 潮b、伊野浩介a、井上(安田)久美b, 末永智一b

ALIGNMENT OF NANO CARBON MATERIALS USING DIELECTROPHORESIS AND
APPLICATION FOR ELECTRODE ARRAY DEVICE

Hitoshi Shikua, Ushio Naitob, Kosuke Inoa, Kumi Y. Inoueb, Tomokazu Matsueb

aGraduate School of Engineering, Tohoku University, Sendai 980-8579
bGraduate School of Environmental Studies, Tohoku University, Sendai 980-8579

Multi-walled carbon nanotubes (MWCNTs) were aligned in hydrogels using dielectrophoresis approach with horizontal and vertical orientation to interdigitated electrode array (IDA) chip. The horizontally aligned MWCNT embedded in PEG-DA hydrogel sheet was used as open-type bipolar electrode (MWCNT-oBPE) array. An external 180 VDC voltage was applied between two electrodes at the distance of 21 mm. The MWCNT-oBPE was set in a buffer solution containing [Ru(bpy)3]Cl2 and tripropylamine (TPA). Electrochemical luminescence was observed to show availability for oBPE-based biosensor. The vertically aligned MWCNT embedded in hydrogel sheet was designed for closed BPE (MWCNT-cBPE). In this case, Thermal gelation with 2,2'-Azobis(isobutyronitrile) (AIBN) was used instead of UV gelation. We confirmed vertical alignment by optical microscope.

To Japanese Contents / To English Contents


29.

シグナリングアレイプローブを用いた病原性微生物の
簡易遺伝子検出法の開発

東京農工大院工a、横河電機株式会社b

○石川万智a、田口朋之b、蓼沼 崇b 吉野知子a、前田義昌a、松永 是a、田中 剛a

DEVELOPMENT OF SIMPLE DETECTION METHOD OF BACTERIAL GENES BY
SIGNALING ARRAY PROBE

Machi ISHIKAWAa, Tomoyuki TAGUCHIb, Takashi TADENUMAb, Tomoko YOSHINOa,
Yoshiaki MAEDAa, Tadashi MATSUNAGAa and Tsuyoshi TANAKA a

anstitute of Engineering, Tokyo University of Agriculture and Technology,
2-24-16, Naka-cho, Koganei, Tokyo 184-8588
bYokogawa Electric Co., 2-9-32 Nakacho, Musashino, Tokyo 180-8750

Detection of microorganisms is crucial in a wide range of industries. Microarray testing is one of the promising methods for this purpose. However, it is time consuming and labor intensive. To address this issue, we established a novel microarray technology using the signaling array probe (SAP). The SAP comprises of fluorescent probe and quench probe, and generates fluorescent signal only when target DNA exists. We demonstrated the detection of two bacterial species, Staphylococcus aureus and Escherichia coli using the SAP-based microarray, on which both single strand- and double strand-DNA could be target DNA and any washing steps were not needed. This method would be useful to detect contaminated microorganisms in a variety of applications.

To Japanese Contents / To English Contents


30.

コロニーフィンガープリントに基づく
簡易・迅速な黄色ブドウ球菌の判別

東京農工大院工a、株式会社マルコムb

○杉山由依a、土橋弘典a、吉野知子a、前田義昌a、林 泰圭b、原田 学b 松永 是a、田中 剛a

COLONY FINGERPRINT-BASED APPROACH FOR RAPID AND EASY
DISCRIMINATION OF STAPHYLOCOCCUS SPECIES

Yui SUGIYAMAa, Hironori DOBASHIa, Tomoko YOSHINOa, Yoshiaki MAEDAa,
Tae-kyu LIMb, Manabu HARADAb, Tadashi MATSUNAGAa, and Tsuyoshi TANAKAa

aInstitute of Engineering, Tokyo University of Agriculture and Technology,
2-24-16, Naka-cho, Koganei, Tokyo 184-8588
bMalcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071

Detection and discrimination of bacteria are crucial in a wide range of industries including production of foods, cosmetics, and pharmaceuticals. Conventional methods for discrimination of bacteria are based on biochemical and/or genetic analyses. However, such methods are time-consuming and labor-intensive. To address this issue, we have developed a novel discrimination methodology using a lens-less imaging system, which is composed of a complementary metal oxide semiconductor (CMOS) sensor, medium agar plate and LED light. This lens-less imaging system has the advantage of wide field of view, enabling simultaneous tracking of the growth of multiple microcolonies. Morphological and growth characters of each microcolony are quantitatively examined by the image analysis, and a set of characteristic parameters (termed colony fingerprint) are extracted. Discrimination of bacteria is performed by multivariate analyses of the colony fingerprint. In this study, we applied this method for discrimination of five closely-related Staphylococcus species (S. aureus, S. epidermidis, S. haemolyticus, S. saprophyticus, S. simulans). Using support vector machine (SVM) technique, and discrimination of theses bacteria was demonstrated with 100% of accuracy. Moreover, discrimination of S. aureus in the mixed culture with other bacterium Pseudomonas aeruginosa was also demonstrated. These results suggest that the colony fingerprint-based method is useful for discrimination of contaminated bacteria with high accuracy and robustness.

To Japanese Contents / To English Contents


31.

POCT指向のための遠心促熱対流型チップPCRの開発と応用

阪大院工

○斉藤真人、高橋和也、三巻拓矢、民谷栄一

POCT-ORIENTED RAPID CHIP PCR DEVICE SYSTEM ASSISTED WITH
CENTRIFUGAL THERMAL CONVECTION AND ITS APPLICATION

Masato SAITO, Kazuya TAKAHASHI, Takuya MITSUMAKI and Eiichi TAMIYA

Graduate School of Engineering, Osaka University,
2-1 Yamadaoka, Suita 565-0871

Drug-resistant enterobacteriaceae is of increasing global concern. Rapid detection and response are required for protecting from its propagation. For this issue, we have developed the Point-of-Care Testing (POCT)-oriented polymerase chain reaction (PCR) device which is driven by centrifugation assisted thermal convection. A cyclo-olefin polymer (COP) microfluidic chip, fabricated by injection molding, was integrated to a device that can control the temperature during chip rotation. The PCR solution could be driven by thermal convection and continuously exchanged high/low temperatures in a ring structured micro-channel without using a typical syringe pump. The flow rate was then able to be controlled by the relative gravity acceleration of the centrifugation device attached to the bottom of micro-channel. The on-chip DNA amplification of Imipenemase-6 (IMP-6), one of the genes responsible for carbapenem-resistance, was detected from 217fg of bacterial total DNA within 15min at 7G. In addition, we have successfully achieved the detection of blaIMP-6 from human stool samples. This result indicated that the easy and rapid PCR could be done without sample pre-treatment and technical proficiency of users.

To Japanese Contents / To English Contents


32.

分子鋳型金ナノ粒子標識を用いた
特定細菌の検出

阪府大院工

○木下隆将、Nguyen Quang Dung、椎木 弘、長岡 勉

DETECTION OF A SPECIFIC BACTERIUM USING MOLECULARLY
IMPRINTED GOLD NANOPARTICLES

Takamasa KINOSHITA, Dung Quang NGUYEN, Hiroshi SHIIGI, and Tsutomu NAGAOKA

Department of Applied Chemistry, Osaka Prefecture University,
Sakai-shi, Osaka 599-8570

An antibody, which can recognizes and binds a specific antigen, is applied for various purposes such as separation, purification, and sensing. Molecularly imprinted polymers (MIP) cannot only mimic the function of antibodies but also expedite and simplify to produce a receptor comparing with antibodies which are produced by immune response. We accomplished to synthesize nanometer-sized composite particle consisting of MIP based on O157-antigenic structure of enterohemorrhagic Escherichia coli (E. coli) O157 and encapsulated gold nanoparticles (Au NPs). The composite bound to E. coli O157 and enhanced light-scattering intensity of the cell owing to the optical properties of the Au NPs. Moreover, the composite shows excellent selectivity against other types of E. coli such as O26 and O rough. This composite as an artificial antibody and an optical label is expected to be applied for the detection of various kinds of bacterium including new threats by imprinting specific antigenic structure depending on bacterial species.

To Japanese Contents / To English Contents


33.

分子インプリント高分子をグラフトした
グラファイト粒子のペースト電極による血中ヘパリンセンシング

芝浦工大

○吉見靖男、八木澤佑人

BLOOD-HEPARIN SENSING USING PASTE ELECTRODE OF
GRAPHITE PARTICLE GRAFTED WITH MOLECULARLY IMPRINTED POLYMER

Yasuo YOSHIMI and Yuto YAGISAWA

Department of Applied Chemistry Shibaura Institute of Technology,
Toyosu, Koto-ku, Tokyo 135-8548

Heparin-imprinted polymer was grafted on graphite particles in fluidized bed reactor and paste electrodes were fabricated with the grafted particles. The electrodes have high homogeneity in heparin-sensitivity. The sensitivity to heparin in blood was same as that in physiological saline. The property is very feasible for disposable sensor for heparin in blood.

To Japanese Contents / To English Contents


34.

脱水素酵素の還元反応を用いたアセトアルデヒドガス用
探嗅カメラ(可視化計測システム)

医科歯科大院a、医科歯科大・生材研b

 ○飯谷健太a、ナセデン ムニラa、佐藤敏征a、當麻浩司b、荒川貴博b、三林浩二a,b

BIO-FLUOROMETRIC IMAGING SYSTEM “SNIFF-CAM” USING ENZYMATIC
REDUCTION FOR GASEOUS ACETALDEHYDE IN HXHALED BREATH

Kenta IITANIa, Munire NAISIERDINGa, Toshiyuki SATOa, Koji TOMAb,
Takahiro ARAKAWAb and Kohji MITSUBAYASHIa,b

aGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
1-5-45 Yushima, Bunkyo-ku, Tokyo 113-0034
bInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,
2-3-10 Kanda-Sugrugadai Chiyoda-ku, Tokyo 101-0062

A bio-fluorometric imaging system (Sniff-cam) exploiting an enzymatic reaction was developed for measurement of gaseous acetaldehyde (AA) in exhaled breath. This system consisted of a highly sensitive camera, an ultraviolet-light emitting diode (UV-LED) array sheet and an alcohol dehydrogenase (ADH) immobilized mesh. Reduced Nicotinamide adenine dinucleotide (NADH) is consumed by the enzymatic reduction of AA with ADH, and its concentration is inverse-proportional to that of gaseous AA. In this system, the concentration of gaseous AA is quantitatively visualized with fluorescence intensities emitted from NADH by UV photo-excitation. The detection limit of 100 ppb with the high-selectivity for AA vapor was demonstrated in the sniff-cam. Finally, the Sniff-cam was applied to measure gaseous AA in exhaled breath after intake of an alcohol beverage, and a fluctuation of AA concentration with time was observed as alcohol metabolism.

To Japanese Contents / To English Contents


35.

CONSTRUCTION OF CELL-IMPRINTED
MICROSPHERE AND ITS APPLICATION FOR SENSOR

Osaka Pref. Univ.

Xueling Shan, Takuya Yamauchi, Hiroshi Shiigi, Tsutomu Nagaoka

Graduate School of Engineering, Osaka Prefecture University,
Sakai, Osaka 599-8570

Specific identification of enterohemorrhagic Escherichia coli was achieved using microspheres coated with overoxidized polypyrrole, and which recognized E. coli O157:H7 based on shape complementarity. It is a micrometer sized core-shell particle, composed of a gold coated core and a polymer complex shell. The thickness of polymer shell was controlled in a suitable level for cells imprinting. The microsphere has an approximative size compared to cells and well dispersed in aqueous media. These features can improve the interaction between microsphere and cells sufficiently, resulting specific, spontaneous, and efficient binding of E. coli O157:H7 through surface area effects. In addition, the microsphere recognized E. coli O157:H7 specifically with a high selectivity (over 10). The microspheres are an easy-to-use tool to detect and concentrate E. coli strains that cause food poisoning.

To Japanese Contents / To English Contents


36.

プラズマ開始重合で形成したモレキュラー
インプリントポリマー膜を用いるヘパリンの選択検出

芝浦工大

○引地 敦、有田智彦、六車仁志、吉見靖男

SELECTIVE DETECTION OF HEPARIN WITH MOLECULAR IMPRINTING POLYMER
MEMBRANE FORMED BY PLASMA INITIATED POLYMERIZATION

Atsushi HIKICHI, Tomohiko ARITA, Hitoshi MUGURUMA, and Yasuo YOSHIMI

Shibaura Institute of Technology

Heparin is the most important anticoagulant drug used during surgeries and extracorporeal therapies. Although the blood levels of heparin should be monitored continuously during the procedure to ensure the safety of the patient, there is currently no technique for measuring heparin in real time. This study describes the use of a molecularly imprinted polymer (MIP) as a recognition element in the development of a heparin sensor for real-time monitoring. An gold electrode grafted with a heparin-specific MIP by plasma-induced polymerization was used as a quartz crystal microbalance (QCM) sensor. The procedure of plasma initiated polymerization is as follows: (i) The gold electrode is treated by hexamethyldisiloxane plasma treatment for 1 min. (ii) The gold electrode solution is pour into the water solution for 12 h. The water solution heparin (template) contains (2-(methacryloxy)-ethyl)trimethylammonium chloride acrylamide (functional monomer), acrylamide, and N,N-methylenebisacrylamide (crosslimker). (iii) The gold electrode is washed by potassium chloride solution so as to remove the template. Non-imprinted polymer (NIP) is carried out like as the same procedure without template. The QCM measurement showed that the heparin-specific MIP bound to heparin and did not bind to chondroitin sulphate C which is heparin analog material. NIP did not bind to both of heparin and chondroitin sulfate C.

To Japanese Contents / To English Contents


37.

ELISA用マイクロ流路と分子インプリントポリマーを利用した
ラット血清中のビスフェノールAセンシング

創価大

○久保いづみ、高士恵太

BISPHENOL A SENSING UTILIZING MICROFLUIDIC DEVICE FOR ELISA AND
MOLECULARLY IMPRINTED POLYMER

Izumi KUBO, Keita TAKASHI,

Soka University
Hachioji, Tokyo, 192-8577

We investigated analytical procedure of bisphenol A based on enzyme linked immunosorbent assay (ELISA) using microfluidic device for ELISA. Formula of competitive immuno assay of bisphenol A in small amount of sample was investigated utilizing anti-bisphenol A antibody modified beads, which was introduced in a reaction chamber on a microchannel of the microfluidic device. To determine Bisphenol A in rat serum, solid phase extraction using molecularly imprinted polymer (MIP) was examined. Using the microfluidic device for ELISA and MIP, Bisphenol A in rat serum was determined in shorter time with much smaller volume of sample than conventional ELISA.

To Japanese Contents / To English Contents


特別講演

マイクロ化学ペンによるナノ・マイクロ化学構造体の作製

首都大学東京

○内山一美

FABRICATION OF NANO / MICRO STRUCTURE BY MICRO CHEMICAL PEN

Katsumi UCHIYAMA

Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University,
Minamiohsawa, Hachioji, Tokyo 192-0397

Various micro/nano surface-modification techniques such as photolithography, dip-pen lithography and micro-fluidic systems have been developed. And they were used to extend the functionalities of solid surfaces. While those approaches work in the “open space”, push–pull systems which work in solutions have recently drawn considerable attention. However, the confining flows performed by push–pull systems have realized only the dispense process, while microscale, region-selective chemical reactions have remained unattainable. This work reports a micro-chemical pen that enables region-selective chemical reactions for the micro/nano surface modification. The chemical pen is based on the principle of microfluidic laminar flows and the resulting mixing of reagents by the mutual molecular diffusion. The micro/nano scale diffusion layer performs as the working region of the surface modification. This report represents the first demonstration of an open micro reactor in which two different reagents react on a real solid sample. The multifunctional characteristics of the micro chemical pen are confirmed by different types of reactions in many research areas, including inorganic chemistry, polymer science, electrochemistry and biological sample treatment.

To Japanese Contents / To English Contents


38.

加齢臭成分ノネナール計測のための
気相用バイオスニファに関する研究

医科歯科大・生材研a、 医科歯科大院b

○荒川貴博a、森 英久b、叶 明a、當麻浩司a、三林浩二a,b

A GAS PHASE BIO-SNIFFER FOR MONITORING OF 2-NONENAL
AS AGING ODOR COMPONENT

Takahiro ARAKAWAa, Hidehisa MORIb, Ming YEa, Koji TOMAa, and Kohji MITSUBAYASHIa,b

aInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University,
2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062
bGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062

Over age 40 people have higher levels of a fragrant organic compound known as 2-nonenal on the skin. In this research, a 2-nonenal bio-sniffer (biochemical gas sensor) was constructed with a fluorometric device and demonstrated for measuring it from the neck skin surface. The bio-sniffer was composed by a flow-cell with an acetaldehyde dehydrogenase (ALDH) immobilized membrane, an optical fiber and a UV-LED excitation light source. NADH would be produced by the ALDH enzymatic reaction of 2-nonenal and the production volume is proportional to the concentration of 2-nonenal. Phosphate buffer, which contained NADH, was circulated into the flow-cell for supplying co-enzyme and removing the excessive substrates and products. The photo multiplier detects the change of the fluorescence intensity emitted from NADH. We also demonstrated that the alter value of fluorescence intensity was correlated with 2-nonenal concentration from 0.4 to 11 ppm. This calibration range includes the concentration of 2-nonenal on the skin of healthy young and old people. Finally, the 2-nonenal bio-sniffer was applied to measure the 2-nonenal of the skin of neck. This highly sensitivity bio-sniffer for 2-nonenal is expected to apply for the evaluation of age-related change in the concentration of body odor.

To Japanese Contents / To English Contents


39.

単層カーボンナノチューブとフラビンアデニンジヌクレオチド依存
グルコースデキドロゲナーゼからなるバイオセンサ

芝浦工大1、産総研2

日高大貴1、○六車仁志1、岩佐尚徳2、平塚淳典2、鵜沢浩隆2

BIOSENSOR WITH SINGLE-WALLED CARBON NANOTUBE AND FLAVIN ADENINE
DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE

Hiroki HIDAKA1, Hitoshi MUGURUMA1, Hisanori IWASA2, Atsunori HIRATSUKA2, and Hirotaka UZAWA2

1Shibaura Institute of Technology,
2 National Institute of Advanced Industrial Science and Technology

The flavoenzymes flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) and oxidase (FAD-GOx) do not undergo direct electron transfer (DET) at conventional electrodes, because the FAD cofactor is buried deeply (~1.4 nm) below the protein surface. We present a mediatorless DET between oxygen-insensitive FAD-GDH and single-walled carbon nanotubes (SWNTs). A glucose-concentration-dependent current (GCDC) is observed at the electrode with the combination of glycosylated FAD-GDH and debundled SWNTs; the GCDC due to an increase in the polarized potential during potential sweep voltammetry increases steeply (+0.1 V of onset, 1.2 mA cm-2 at +0.6 V 48 mM glucose) without the appearance of the FAD redox peak at –0.45 V. In the control experiment, the GCDC is not observed at the counterpart with debundled multi-walled carbon nanotubes (MWNTs). In the control experiment, the GCDC is observed at an analogous electrode based on oxygen-sensitive FAD-GOx with both of SWNT and MWNT in the presence of oxygen because oxygen acts as a natural and mobile mediator. Therefore, observation of the GCDC at the electrode with oxygen-insensitive FAD-GDH and debundled SWNTs provides evidence of mediatorless DET, even though oxygen is present. Details of the DET are discussed with respect to the recently reported crystallographic model of FAD-GDH. The three-dimensional globular FAD-GDH molecule is 4.5×5.6×7.8 nm, which is larger than the 1.2-nm diameter of an individual SWNT and smaller than the 10-nm diameter of an individual MWNT. Only individual SWNTs can be plugged into the groove of FAD-GDH, which is close to and within 1.0-nm of FAD, while maintaining their catalytic activity. Images obtained using transmission electron and atomic force microscopies support the stated configuration of FAD-GDH molecules and debundled SWNTs. We demonstrate that DET can be explained by quantum tunneling theory.

To Japanese Contents / To English Contents


40.

化学修飾済みの炭素繊維に固定化した
酵素を用いるフロー計測と諸特性

神奈川工科大

○佐藤生男、菊地大介、神崎 愷

FLOW MEASURMENT AND ITS CHARACTERISTICS USING ENZYMES
IMMOBILIZED ONTO CARBON FIBERS CHEMICALLY MODIFIED

Ikuo SATOH,Daisuke KIKUCHI, and Yasushi KANZAKI

Department of Applied Chemistry, Faculty of Engineering,
Kanagawa Institute of Technology,
1030 Shimo-Ogino, Atsugi Kanagawa, 243-0292, Japan

Preparation methods of immobilized alkaline phosphatase onto carbon fibers chemically treated were tried. The immobilized preparations were packed into a plastic column and then,this column was set into a flow-injection system. The system was applied to a flow-injection determination of zinc(II) ions based on an apoenzyme reactivation method. The performance characteristics of the flow system were reported.

To Japanese Contents / To English Contents


41.

間接競合阻害法による二次抗原抗体反応の速度論的評価

北大院環境科学a、ウシオ電機b、北大国際連携教育局c

○Dulal KABIRAZa、森田金市b、○川口俊一a,c

KINETIC STUDY OF SECONDARY IMMUNOREACTION
IN INDIRECT COMPETITIVE INHIBITION IMMUNOASSAY

Dulal Kabiraza , Kinichi Moritab, Toshikazu Kawaguchia,c

aGraduate School of Environmental Science, Hokkaido University,
Sapporo-shi, Hokkaido 060-0810
bUSHIO INC. Tokyo 100-8150
cInstitution for Collaborative Research and Education, Hokkaido University,
Sapporo-shi, Hokkaido 060-8589

Surface Plasmon Resonance (SPR) biosensor has been studied in our group for a practical application in small molecule detection. In this study, the indirect competitive inhibition immunoassay with secondary immunoreaction was investigated. It was known that the low affinity constant sensor surface provided the high sensitivity in the primary immunoreaction. Thus, it is inferred here that the affinity constant of the sensor surface also influences on the secondary immunoreaction. Past studies reported that the sensitivity of an immunoassay determined by the affinity constant of the immunosurface. Therefore, a low affinity and a high affinity sensor surface were fabricated for the kinetic study of the secondary immunoreaction. Aiming to achieve a high SPR response and a high sensitiveity, AuNP labeled secondary Ab was also used for the comparisonin in the secondary immunoreaction. A high response and a low limit of detection were found in the high affinity sensor surface, because it could be considered that the secondary antibody bonds to the tail of primary antibody.

To Japanese Contents / To English Contents


42.

有機トランジスタ型イムノセンサによる
ヒトクロモグラニンAのラベルフリー検出

東大生研a、山形大b、産総研c,d、埼玉工大e

○南 豪a、南木 創a、佐々木由比a,b、脇田慎一c、栗田僚二d、丹羽 修e、時任静士b

LABEL-FREE DETECTION OF HUMAN CHROMOGRANIN A (HCGA) USING AN
ORGANIC TRANSISTOR-BASED IMMUNOSENSOR

Tsuyoshi MINAMIa, Tsukuru MINAMIKIa, Yui SASAKIa,b, Shin-ichi WAKIDAc,
Ryoji KURITAd, Osamu NIWAe, Shizuo TOKITO b

a Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505
bGraduate School of Science and Engineering, Yamagata University,
Yonezawa, Yamagata 992-8510
cNational Institute of Advanced Industrial Science and Technology (AIST),
Ikeda, Osaka 563-8577
dNational Institute of Advanced Industrial Science and Technology (AIST),
Tsukuba, Ibaraki 305-8566
e Advanced Science Research Laboratory, Saitama Institute of Technology,
Fukaya, Saitama 369-0293

Chromogranin A (CgA), which is one of the glycoproteins, is co-stored and co-released with catecholamines from storage granules in the adrenal medulla, or with the parathyroid gland. Although CgA is known as an important biomarker for monitoring in several diseases or psychophysical stress, the development of biosensor platforms for CgA detection is in their early stages. Toward that end, we decided to fabricate an organic field-effect transistor (OFET)-based immunosensor for human CgA (hCgA). The fabricated OFET device has an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics. The sensitivity and selectivity suggest that the OFET-based immunosensor is potentially applied to the rapid detection of the glycoprotein concentration without any labeling.

To Japanese Contents / To English Contents


43.

電解二酸化マンガンを用いる非酵素型
グルコースセンサの作製と評価

東京理科大学

○堀場達雄、渡邊健介、半田裕、井上正之、駒場慎一

FABRICATION AND EVALUATION OF NON-ENZYME TYPE GLUCOSE SENSORS
USING ELECTROLYTIC MANGANESE DIOXIDE

Tatsuo Horiba, Kensuke Watanabe, Yutaka Handa, Masayuki Inoue,
and Shinichi Komaba

Tokyo University of Science, 1-3, Kagurazaka Shinjuku, Tokyo 162-8601

We have been studying on non-enzyme type glucose sensors by using electrolytic manganese dioxide (EMD) as electrocatalyst, which do not require any expensive glucose oxidizing catalysts such as glucose oxidase or precious metal like platinum. However, the performance of the EMD electrode as glucose sensors is still not satisfactory to be substituted for the conventional sensors of enzyme electrode. In this study, glucose detectability of the EMD electrode is improved by adding surfactants into electrolytic bath for EMD preparation. Polyion complex coating on the EMD electrode is efficient to eliminate interference response to ascorbic and uric acids. Moreover, anodic oxidation mechanism of glucose is discussed with consideration of equilibrium of structural transition of glucose in aqueous solution.

To Japanese Contents / To English Contents