Vol. 31, Supplement B (2015)

Proceedings of the 59th Chemical Sensor Symposium
Fukaya, Saitama, September 11-12, 2015

 

Abstracts



1.

電解二酸化マンガン修飾電極によるグルコース電解酸化とセンサへの応用

東京理科大

○渡邊健介、山際 清史、半田 裕、駒場 慎一

ELECTROCATALYTIC OXIDATION OF GLUCOSE AT ELECTROLYTIC MANGANESE
DIOXIDE MODIFIED ELECTRODE AND ITS APPLICATION TO AMPEROMETRIC
GLUCOSE SENSOR

Kensuke WATANABE, Kiyofumi YAMAGIWA, Yutaka HANDA, Shinichi KOMABA

Department of Applied Chemistry, Tokyo University of Science, 1-3 Kagurazaka,
Shinjuku, Tokyo 162-8601

We have developed non-enzymatic glucose oxidation electrode composed of electrolytic manganese dioxide (EMD). By using the EMD modified electrode, glucose is electrochemically oxidized in analyte with near neutral pH (pH 8.0) due to its electrocatalytic ability. In order to study its detailed electrochemical properties, effects of temperature and pH of analyte on the glucose oxidation were investigated for an application to glucose sensor. Furthermore, we also prepared polyion complex (PIC)-coated EMD electrode to improve electrochemical stability and sensitivity for detecting glucose. From these results, we will discuss detailed mechanism of glucose oxidation at EMD modified electrode.

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2.

二種酵素固定化カーボンフェルトを利用する
FIA式グルコースバイオセンサの開発

埼玉工大a、遼寧科技大b

渡辺崇史a、王 月b、○長谷部 靖a

DEVELOPMENT OF FIA-TYPE GLUCOSE BIOSESOR
USING BI-ENZYMES-IMMOBILIZED CARBON-FELT

Takashi WATANABEa Yue WANGb and Yasushi HASEBEa

aDepartment of Life Science and Green Chemistry, Saitama Institute of Technology,
Fukaya, Saitama 369-0293, Japan
bSchool of Chemical Engineering, University of Science and Technology Liaoning,
Anshan, Liaoning 114501, China

Glucose oxidase (GOx) and Horseradish Peroxidase (HRP) were physically adsorbed onto porous carbon felt (CF), which is a microelectrode ensemble of micro-carbon fiber and possesses a three-dimensional random structure. HRP and thionine (TN) were firstly adsorbed onto the CF, and then, the GOx was further adsorbed onto HRP/TN-adsorbed CF (step-wise adsorption). The resulting GOx/HRP/TN-adsorbed-CF was successfully used as an amperometric detector for FIA-type biosensor for glucose. Enzyme-adsorption conditions and operational conditions were optimized. The developed biosensor showed the sensitivity (2.076 μA/mM), the limit of detection (0.014 mM), and linear range (0.03 ~ 3 mM). Interference by ascorbic acid was able to cancel by the control of applied potential. The RSD for 63 consecutive injection of 10 mM glucose was 3.71 %. The resulting GOx/HRP/TN-CF-based FIA biosensor was applied for the determination glucose in various beverages, and the analytical results obtained by the sensor was fairly good agreement with those by the conventional spectrophotometry.

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3.

体内グルコースモニタリング用低侵襲型センサの作製およびその評価

徳島大a、産総研b

○安澤幹人a、李 江a、渕脇雄介b、田中正人b

PREPARATION AND EVALUATION OF LOW-INVASIVE SENSOR FOR IN VIVO
GLUCOSE MONITORING

Mikito YASUZAWAa, Jiang Lia, Yusuke FUCHIWAKIb and Masato TANAKAb

aDepartment of Chemical Science and Technology, Tokushima University,
Tokushima-shi 770-8506
bHealth Research Institute, National Institute of Advanced Industrial Science and Technology
Takamatsu-shi, Kagawa 761-0395

A fine needle type glucose sensor, which has sensing region on the tip of a fine tapered electrode, was prepared using tapered PEEK tube with platinum-iridium alloy wire. Glucose oxidase was immobilized on the surface of Pt-Ir electrode using the combination of electrodeposition and electropolymerization procedures. The obtained sensor showed good response with sufficient linear relationship between response current and glucose concentration. Long-term stability of the obtained electrode was evaluated for 35 days. After initial change of response current for a week, constant response current was obtained for 1 month and approximately 80% of the initial sensor response remained. In vivo measurement evaluation of the electrode was performed using rabbit. Considerable glucose sensor response change due to the trend of glucose concentration was observed.

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4.

配向性を有する固定化組換えβ-セクレターゼとフローシステムを用いた抗β-セクレターゼ探索系の構築

神奈川工大・応用バイオ科学科a、応用化学科b

○飯田泰広a、白石有希a、篠原博宣a、山口淳一b

CONSTRUCTION OF SCREENING SYSTEM FOR β-SECRETASE INHIBITOR
BY USING ORIENTATED IMMOBILIZING RECOMBINANT β-SECRETASE IN
COMBINATION WITH A FLOW SYSTEM

Yasuhrio IIDAa, Yuki SHRAISHIa, Hironobu SHINOHARAa and Jyunichi YAMAGUCHIb

aDepartment of Applied Bioscience, Faculty of Applied Bioscience, Kanagawa Institute of Technology
1030 Shimo-Ogino, Atsugi, Kanagawa-ken, 243-0292
b Department of Applied Bioscience, Faculty of Applied Chemistry, Kanagawa Institute of Technology
1030 Shimo-Ogino, Atsugi, Kanagawa-ken, 243-0292

Alzheimer's disease ( AD ) is one of the type of dementia. -Secretase is known as one of a key enzyme for development of therapeutic agent to AD because the amyloid plaque causing AD was produced by the catalysis of the enzymes. Several inhibitors to -secretase was known and the effects of the inhibitors are also known as a reversible and competitive inhibitors. In the previous study, we already developed the system to evaluate properties of the inhibitors whether its effect is reversible or irreversible. In this study, we developed evaluation system for activity of -secretase by using immobilized recombinant -secretase in combination with a FIA (Flow Injection Analysis) system. On the other hand, conventional method for enzyme immobilization cannot control enzyme orientation. Hence we investigated orientation of immobilized enzyme by using specific binding of biotin and streptavidin. And also we tried to miniaturize to system for minimize of material and reduce assay time by micro flow channel.

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5.

抗酸化能評価用センサの開発

東京工大

〇後藤正男、中山善皓、永井邦彦

CHEMICAL SENSOR FOR TOTAL ANTIOXIDANT CAPACITY ASSAY

Masao Gotoh, Yoshihiro Nakayama and Kunihiko Nagai

School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1
Katakura, Hachioji, Tokyo 192-0982, Japan

A carbon screen–printed disposable microchip as a microstructured two-electrode system was used for the establishment of a chronoamperometric total antioxidant capacity (C-TAC) assay of foods and drinks. The electrochemical response to an antioxidant was indirectly determined through mediator, and was strongly affected by the solubility of both the mediator and the antioxidant in the measurement solution. By intensive investigation, it was found that methanol was the best solvent for the measurements and food extraction. Under optimum conditions for the C-TAC assay, micro-volume measurement of 30 µL was realized using the present microchip, and calibration curves for representative antioxidants were successfully obtained with excellent determination coefficients. Finally, the conventional DPPH test using foods and drinks was applied for comparison with the present C-TAC method, and the obtained results showed good correspondence.

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6.

フェロセン修飾分子インプリント高分子によるバンコマイシンのリエージェントレスセンシング

芝浦工大

○吉見 靖男、大平浩史

REAGENTLESS SENSING OF VANCOMYCINE USING MOLECULARLY IMPRINTED
POLYMER INCLUDING FERROCENE

Yasuo YOSHIMI, Hirofumi OHIRA

Department of Applied Chemistry, Shibaura Institute of Technology,
Toyosu, Tokyo, 135-8548

Currently, vancomycin is primarily used to treat serious infections caused by gram-positive bacteria which are known or suspected to be resistant to other antibiotics. But vancomycin has a serious nephrotoxicity, and then the monitoring of the level of vancomycin in serum is most strongly proposed. The purpose of the present study is a development of reagentless sensor for monitoring vancomycin using molecularly imprinted polymer. Vinylferrocene, methacrylic acid, acrylamide and methylenebisacrylamide were copolymerized with grafting onto indium-tin oxide (ITO) electrode in the presence of vancomycin as a template. Differential pulse voltammetry was performed with the grafted electrode in buffered saline solution. The current at 0.8 V vs. Ag/AgCl was sensitive to the vancomycin concentration between 0-50 µM however was unstably sensitive to teicoplanin which has similar structure to vancomycin. The electrode prepared without template (non-imprinted electrode) was insensitive to vancomycin. As a result, an electrode grafted with vancomycin-imprinted polymer including ferrocenyl group can work as a reagentless sensor for vancomycin. It would be helpful for monitoring of vancomycin.

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7.

分子インプリント固定電極による全血中ヘパリン濃度の安定した測定法の検討

芝浦工大a、泉工医科工業b

○吉見靖男a、稲葉 良a、小川貴也a、井上 将b、桑名克之b

STABILIZED SENSING OF HEPARIN IN WHOLE BLOOD BY AN ELECTRODE GRAFTED
WITH HEPARIN-IMPRINTED POLYMER

Yasuo YOSHIMIa, Ryo IANABAa, Takaya OGAWAa, Masaru INOUEb and Katsuyuki KUWANAb

aDepartment of Applied Chemistry, Shibaura Institute of Technology
Toyosu, Tokyo, 135-8548
bSenko Medical Instrument Mfg. Co., Ltd.
2-10-1 Hamakawado, Kasukabe, Saitama, 344-0054

A real-time heparin sensor is required to optimize dosage of heparin for extracorporeal circulation and its antidote protamine. Then, we are trying to develop a heparin sensor using a molecularly imprinted polymer (MIP). In the present work, we studied the method to stabilize measurement of heparin concentration in whole blood by using the MIP-grafted electrode. An initiator of radical polymerization was introduced on an indium-tin oxide (ITO) electrode. Heparin sodium, methacryloxethyltrimethoxysilane, and acrylamide were dissolved in water. Methylenebisacrylamide was dissolved in dimethylformamide. Mixture of the both of the solutions was introduced into the gap between the surfaces of quartz crystal plate and the treated ITO electrode. Ultraviolet was irradiated to the surface of the ITO to graft copolymer of the monomers. The ITO was washed with aqueous solution of 1 M sodium chloride to remove the template and to obtain the MIP-grafted electrode. Cyclic voltammetry was performed with the MIP-grafted electrode in physiological saline or bovine whole blood including 0-8 unit/mL heparin and 5 mM ferrocyanide as a redox marker. As the result, the current intensity was decreased by the increase of heparin concentration either in the saline or the whole blood. However, the sensitivity to heparin in the blood was approximately 50% of that in the saline. The heparin-sensitivity of the electrode coated with SEC-1® (Toyobo) in the blood was 74% of that in saline. As another approach, uncoated-electrode was washed at the interval of measurement in blood with protease-containing detergent. The heparin-sensitivity in the blood with the washed electrode was 77% of that in the saline.

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8.

電解改質カーボンフェルト電極を用いた
残留塩素のバッチインジェクションクーロメトリー

バイオニクス機器a、埼玉工大院工b、埼玉工大工c

○佐藤雅利a、赤部周平b、廣田孝範a、藤原直義a、松浦宏昭b,c、内山俊一b

BATCH INJECTION COULOMETRY OF RESIDUAL CHLORINE USING
ELECTROLYTIC MODIFIED CARBON FELT ELECTRODES

Masatoshi SATOa, Syuhei AKABEb, Takanori HIROTAa, Naoyoshi FUJIWARAa,
Hiroaki MATSUURAb,c and Shunichi UCHIYAMAb

aBionics Instrument Co., Ltd., Shimizu, Higashiyamato, Tokyo 207-0004
bDepartment of Applied Chemistry, Graduate School of Engineering,
Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293
cDepartment of Life Science and Green Chemistry, Faculty of Engineering
Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293

We developed an absolute determination method of residual chlorine using an electrolytic modified carbon felt (EMCF) electrode as a working electrode. The EMCF electrode was fabricated by stepwise electrolysis in ammonium carbamate solution and sulfuric acid. The electrochemical behavior of the EMCF electrode was measured by using cyclic voltammetry. The reduction wave of residual chlorine began from 0.8 V vs. Ag/AgCl in a neutral medium. A good linear relationship was obtained in the concentration range of 0.13 to 788,000 mg L-1 Cl residual chlorine. The relative standard deviation for tenth successive measurements was less than 1.3 % for 130 mg L-1 Cl. The measurement of residual chlorine was finished completely in a short time(~60 sec.). The electrical charge obtained by measurement of residual chlorine was not dependent on the pH region from 2 to 8. This indicated that our proposed method can be used for the measurement of the residual chlorine concentration.

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清山賞受賞講演1

光ファイバ分布型水素センサの研究

横浜国立大

岡崎慎司

DEVELOPMENTS OF FIBER-OPTIC GAS SENSORS FOR DISTRIBUTED
HYDROGEN GAS LEAKAGE DETECTION SYSTEM

Shinji OKAZAKI

Graduate School of Engineering, Yokohama National University, Tokiwadai,
Hodogaya-ku, Yokohama 240-8501

Our recent developments of fiber-optic hydrogen gas sensors based on evanescent-wave absorption and Bragg wavelength shift are introduced. A platinum-loaded tungsten trioxide (Pt/WO3) thin film as a hydrogen sensitive material for the evanescent-wave type sensor was successfully immobilized on the periphery of optical fiber core by sol-gel method. The sensor could respond to hydrogen gas and the reproducibility was good. A distributed sensing performance using optical time domain reflectometry were demonstrated. Secondary, the fiber-Bragg grating (FBG) hydrogen gas sensor based on catalytic-heat is described. The sensor device where a platinum-loaded silica (Pt/SiO2) film was immobilized on the periphery of the FBG portion exhibited rapid response. These sensor devices would have a great potential for applying to distributed monitoring of hydrogen leakage from equipment related to hydrogen energy system.

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清山賞受賞講演2

2次元フォトセンサを用いた遺伝子診断・
細胞診断用バイオセンシングシステムの開発

東京農工大

田中 剛

DEVELOPMENT OF BIO-SENSING DEVICES FOR GENETIC AND CELLULAR
DIAGNOSIS USING TWO-DIMENSIONAL PHOTOSENSOR

Tsuyoshi TANAKA

Institute of Engineering, Tokyo University of Agriculture & Technology, Koganei,
Tokyo 184-8588

Electrical and electrochemical sensing systems have been employed in biosensors due to the ease of integration of electrodes and control circuits on the miniaturized devices. In contrast, usage of photo-sensing systems developed with advanced optical components (e.g., condensing lenses and optical filters) has been hampered duet to the difficulties in miniaturization and cost-cutting. However, recently, those limitations are going to be overcome owing to the diffusion of digital cameras and cell phone, in which two-dimensional (2D) photosensors are equipped as imaging devices. Consequently, novel biosensors using 2D photosensors with microfabrication and microfluidic technologies are emerging. Those sensors are free from bulky optical systems (e.g., objective lenses), instead, improve light condensing efficiency on the photosensors to complement low detection sensitivity. Those technologies can contribute to miniaturization of photosensing-based biosensors. In this article, biosensing technologies using 2D photosensors for single-nucleotide polymorphism (SNP) detection based on DNA microarray and cell analysis are introduced.

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9.

亜鉛錯体プローブを用いたエンドトキシン
のLALフリー電気化学検出

産総研a、JNCb

○吉岡恭子a、加藤 大a、鈴木祥夫a、鎌田智之a
佐々木修治b、戸所正美b、丹羽 修a

LAL-FREE ELECTROCHEMICAL DETECTION OF ENDOTOXIN USING ZINC
COMPLEX-BASED PROBES

Kyoko YOSHIOKAa, Dai KATOa, Yoshio SUZUKIa, Tomoyuki KAMATAa, Shuji SASAKIb,
Masami TODOKOROb and Osamu NIWAa

aNational Institute of Advanced Industrial Science and Technology,
Tsukuba, Ibaraki 305-8566
bJNC Corporation, 5-1, Okawa, Kanazawa-ku, Yokohama, 236-8605

We have recently developed an electrochemical endotoxin (ET) detection system by using a combination of an ET-affinity microparticle (CellufineTM ETclean), a zinc complex-based probe for ET detection (ET probe), and an ultraflat nanocarbon film electrode. We have used cetylpyridinium molecule as an ET recognizing part of the ET probe and achieved highly sensitive ET detection (LOD=0.2 ng/mL) by the anodic stripping voltammetry of zinc ion from the captured ET probes. In this report, we applied the already reported ET recognizing peptides (Li5-025 and Sushi peptide) for this ET detection system. We have confirmed ET-affinity of these peptides by surface plasmon resonance measurement. The poly-ε-lysine modified sensor chip captured ET molecules in sample solution and the peptides recognized the adsorbed ETs. However, it was found that ET probes containing these peptides tended to adsorb non-specifically to the poly-ε-lysine membrane and the surface of ETclean microparticle, and therefore, high background signal (noise) was observed. We used the detergent (Tween20) and blocking reagent in the measurement solution to reduce noise and achieved higher S/N ratio in the ET detection system.

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10.

血中循環腫瘍細胞の迅速かつ高精度検出に向けた
広視野一括撮像システムの構築

東京農工大

○高井香織、吉野知子、根岸 諒、佐伯達也、前田義昌、松永 是、田中 剛

RAPID CIRCULATING TUMOR CELL DETECTION BASED ON WIDE-FIELD
FLUORESCENT IMAGING SYSTEM USING 2D PHOTOSENSOR

Kaori TAKAI, Tomoko YOSHINO, Ryo NEGISHI, Tatsuya SAEKI, Yoshiaki MAEDA,
Tadashi MATSUNAGA and Tsuyoshi TANAKA

Institute of Engineering, Tokyo University of Agriculture and Technology,
Koganei, Tokyo 184-8588

Circulating tumor cells (CTCs) are defined as tumor cells circulating in the peripheral blood of patients with metastatic cancer. The number of CTCs has prognostic value in patients and can be used to evaluate therapeutic effects. Recently, we have developed a microcavity array (MCA) (6 mm × 6 mm, Hitachi Chemical Co. Ltd.) system for highly efficient enrichment of CTCs based on differences in cell size and deformability. However, microscopic scanning is required to observe the entire area of a MCA, resulting in lower throughput of image analysis. If the CTCs trapped on microfluidic devices are imaged by one-shot, rapid detection of CTCs is possible. In this study, a wide-field imaging system using two dimensional (2D) complementary metal oxide semiconductor (CMOS) photosensor was developed and combined with the microcavity array system.

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11.

金ナノアンテナ構造の電場増強を用いた高感度蛍光センシング

筑波大

○鈴木駿介、鈴木博章、横川雅俊

GOLD NANOANTENNA FOR HIGHLY SENSITIVE FLUORESCENCE SENSING

Shunsuke SUZUKI, Hiroaki SUZUKI, Masatoshi YOKOKAWA

Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8573

A gold nanoantenna structure was used to enhance the electric field in a gap region between two connected gold nanorods (GNRs) for fluorescence analyses. The position and orientation of the GNRs could be controlled by taking advantage of the properties of crystal structure of the GNR and by solid phase synthesis to improve detection sensitivity of molecules for many biochemical analyses. To obtain nanoantenna structures consisting of GNRs of predetermined numbers, the GNRs were sequentially coupled to the growing GNR chain immobilized on microbeads, which was confirmed by SEM observation. Fluorescence from a dye in the gap of the nanoantenna was enhanced significantly even with weak excitation light compared with the vicinity of single GNRs.

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12.

ストレス計測評価用バイオセンシングデバイスの研究開発(II)
唾液硝酸イオン分泌機序の基礎検討

産総研a、日本分析専門b、神戸大院海事c

○脇田 慎一a、渕脇雄介a、宮道 隆b、北村健一c、村井康二c、林 祐司c

R & D OF BIOSENSING DEVICES FOR STRESS MONITORING(II)
BASIC STUDY ON SECRETORY MECHANISM OF SALIVARY NITRATE FOR
AUTONOMIC NERVOUS SYSTEM RESPONSE TO STRESS

Shin-ichi WAKIDAa, Yuskuke FUCHIWAKIa, Takashi MIYADOb, Kenichi KITAMURAc,
Koji MURAIc, Yuji HAYASHIc

aNational Institute of Advanced Industrial Science and Technology (AIST),
Hayashi 2217-14, Takamatsu, Kagawa 761-0395
bCollege of Analytical Chemistry,
Tenma 2-1-8, Kita-ku, Osaka 565-0043, Japan.
cGraduate School of Maritime Sciences, Kobe University
Fukae-minami 5-1-1, Higashinada, Kobe 658-0022

To carry out research and development on new stress-sensing devices for stress science study, we have investigated several microfluidic POCT (point of care testing) systems and FET biosensors for measurement of salivary stress-marker candidates. During the fundamental studies on the prototype of stress-sensing devices, such as electrophoretic microfluidics and ISFETs (ion-selective field-effect transistors), for real salivary samples of healthy volunteers, we found salivary nitrate might be a biomarker candidate on the autonomic nervous system response to stress. To make a scientific study on the stress response mechanism of salivary nitrate, we investigated simultaneous monitoring for healthy volunteers on mental workload for events of a ship handling using wearable heart rate monitor and salivary nitrate sampling measurement by prototype of salivary nitrate checkers based on NO3--ISFETs using direct potentiometry. We preliminary discussed the secretion mechanism of salivary nitrate for autonomic nerve system response to stress.

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13.

SURFACE PLASMON RESONENCE IMMUNOSENSOR
USING AU-NANOPARTICLE MODIFIED ANTIBODY

北大a、ウシオ電機b

○Dulal Chandra KABIRAZa、森田金市b、川口俊一a

Dulal Chandra KABIRAZa, Kinichi MORITAb, Toshikazu KAWAGUCHIa

aGraduate School of Environmental Science, Hokkaido University,
Sapporo-shi, Hokkaido 060-0810
bUshio Inc., Tokyo 100-8150

An amplification method for surface plasmon resonance (SPR) signal has been studied in this research. Since our target clenbuterol (CLB) is a small molecule, indirect competitive inhibition method is employed. However, the signal change was very small because this method monitored the initial process of immunoreaction. In order to enhance the signal change, a use of Au nanoparticle is devised in the sensing. As known, Au nanoparticle causes the further electromagnetic wave oscillation at the interface. It is so-called localized surface plasmon resonance (LSPR). In this study, we compared the sensing performance between the primary and the secondary antibody (Ab) labeled with 40 nm Au nanoparticle (NP). It was noticed that Au NP bonded to the primary Ab caused 400% signal amplification. Furthermore, the secondary Ab-Au conjugate provides tremendous signal amplification (1200%) and further improvement in the limit of detection of 29 fg/mL (ppq).

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14.

ガルバニ作用に基づく結露応答センサ

物質・材料研究機構

○川喜多仁、知京豊裕、生田目俊秀、大井暁彦、大木知子

DEW-RESPONSE SENSOR BASED ON GALVANIC ACTION

Jin KAWAKITA, Toyohiro CHIKYOW, Toshihide NABATAME, Akihiko OHI, Tomoko OHKI

National Institute for Materials Science
Tsukuba, Ibaraki 305-0044

Environmental water such as moisture, mist, steam rain drops, etc gives critical effect on manufacturing of products, growing of plants, their logistics and storage, infrastructure corrosion, mold generation and so on. In order to develop environmental water sensor superior to conventional humidity sensors, a new sensor based on galvanic action, which electric current flows through the condensed water or dew as an electrolytic solution between different metals, was made by using a semiconductor microfabrication technology involving the photolithography, the metal deposition and the lift-off processes in this study. This sensor showed a high-speed response, high sensitivity and low power consumption.

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15.

バイポーラー電極と駆動電極を用いた
アンペロメトリー計測に関する検討

東北大a、東北大WPI-AIMRb

○井上(安田) 久美a、高野真一朗a、池川未歩a、伊野浩介a、珠玖 仁a、 末永智一a,b

A STUDY FOR AMPEROMETRIC MEASUREMENT USING A CLOSED BIPOLAR
ELECTRODE SYSTEMS

Kumi Y. Inouea, Shinichiro Takanoa, Miho Ikegawaa, Kosuke Inoa, Hitoshi Shikua,
and Tomokazu Matsuea,b

aGraduate School of Environmental Studies ,Tohoku University, Sendai, Miyagi 980-8579
bAdvanced Institute for Materials Research, Tohoku University, Sendai, Miyagi 980-8577

We conducted a basic study to construct an arrayed amperometric measurement method using closed bipolar electrode system (cBPES). In a limiting cell and an excess cell of cBPES, 0.5 mM p-aminophenol (pAP) and 1 mM ferrocyanide /1 mM ferricyanide mixture solution were prepared, respectively. As a bipolar electrode, electrically connected two Au electrodes with 1.6 mm in diameter were inserted into each cell. A driving electrode in the excess cell was connected to a connector of a potentiostat for working electrode (WEc). Another driving electrode and an Ag/AgCl reference electrode in the limiting cell were connected to a connector for a counter (CEc) and a reference electrode, respectively. Using this cBPES, we obtained a cyclic voltammogram reflecting a redox reaction of pAP on the bipolar electrode. Next, we connected two cBPES in order to test a primitive arrayed cBPES. Even if two driving electrodes of two cBPES were connected each other with one electrical lead connected to the WEc, only pAP reaction proceeding in one cBPES was monitored. This indicates that by switching the driving electrode connected to the CEc, amperometric measurements in multiple cBPES can be performed with one electrical lead for WEc.

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16.

乳酸脱水素酵素阻害剤スクリーニングセンサの開発

富山大

○竹津寛人、篠原寛明、須加 実

DEVELOPMENT OF ELECTROCHEMICAL SENSOR FOR SCREENING LACTATE
DEHYDROGENASE INHIBITOR

Hiroto TAKETSU, Hiroaki SHINOHARA and Minoru SUGA

Graduate School of Science and Engineering, University of Toyama
3190 Gofuku, Toyama 930-8555, Japan

In recent years, it has been reported that apoptosis of cancer cells is induced by the inhibition of intracellular lactate dehydrogenase (LDH). Therefore, the development of rapid screening sensors for LDH inhibitors is required. Here, an electrochemical sensor which can quickly and easily evaluate the ability of a LDH inhibitor is proposed. LDH catalyzes the reduction of pyruvate to lactate in the presence of NADH in neutral pH solution. Then the consumption of NADH by this enzymatic reaction was measured by differential pulse voltammetry (DPV) with a glassy carbon disk electrode (GCE). The rate of enzymatic consumption for NADH was investigated in the presence and absence of oxamic acid, which was already known as a LDH inhibitor. The results suggested that the electrochemical enzyme sensor measurement was available to screen LDH inhibitors.

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17.

修飾した炭素繊維への生体触媒の固定化とフローセンサへの適用

神奈川工大

○佐藤生男、神崎 愷

IMMOBILIZATION OF BIOCATALYSTS ONTO CARBON FIBERS
MODIFIED CHEMICALLY AND THEIR APPLICATION TO A FLOW SENSOR

Ikuo SATOH and Yasushi KANZAKI

Department of Applied Chemistry, Faculty of Engineering,
Kanagawa Institute of Technology
1030 Shimo-Ogino, Atsugi, 243-0292, Japan

Immobilization of biocatalysts onto carbon fibers modifiedchemically was investigated. Each of a disk type of theimmobilized enzymes was applied to a flow sensor and then,the performance characteristics of the systems were performed.

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18.

金-酸化チタン光触媒を用いたグルタチオンの選択的検出

東京理科大a、総合研究機構光触媒国際研究セb

○倉賀野朝子a,b、片岸賢翼a,b、A. Devadossb、P. Sudhagarb、寺島千晶b、中田一弥a,b
近藤剛史a,b、四反田 功a,b、板垣昌幸a、湯浅 真a,b、藤嶋 昭b

SELECTIVE DETECTION OF GLUTATHIONE USING Au-TiO2 PHOTOCATALYST

Asako Kuraganoa,b, Kensuke Kataghishia,b, Anitha Devadossb, P. Sudhagarb,
Chiaki Terashimab, Kazuya Nakataa,b, Takeshi Kondoa,b, Isao Shitandaa,b,
Masayuki Itagakia, Makoto Yuasaa,b, Akira Fujishimab

aFaculty of Science and Technology & bPhotocatalysis International Research Center,
Tokyo University of Science, Japan
Noda-shi, Yamazaki, 278-8510

Photoelectrochemical sensing has become a promising analytical tool for the detection of biomolecules owing to its high sensitivity with low background noise. The physiological glutathione concentrations are an excellent indicator of various disease risks. Here, we report Au nanoparticles decorated TiO2 nanocomposites show higher sensitivity and selectivity towards glutathione detection by the unique catalytic activity of Au nanoparticles. Electrospun nanostrucure of Au-TiO2 nanocomposites were coated on FTO substrates followed by annealing at 500C. Upon glutathione addition to phosphate buffer, significant increase in current density was observed for Au-TiO2 electrode than without glutathione. The photocurrent response of Au-TiO2 electrode shows selectivity towards glutathione over ascorbic acid and glucose at 0.33 V (vs. Ag/AgCl) than TiO2 electrodes. The Au-TiO2 nanocomposite electrodes showed a higher sensitivity with linear range between 0.0125 mM and 0.5 mM, which can be ascribed to the higher catalytic activity and strong interactions of Au nanoparticles with glutathione.

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19.

化学センシングのためのバブル型双方向マイクロポンプ

筑波大

○小幡寛隆、横川雅俊、鈴木博章

BUBBLE-BASED BIDIRECTIONAL MICROPUMP FOR CHEMICAL SENSING

Hirotaka OBATA, Masatoshi YOKOKAWA, and Hiroaki SUZUKI

Graduate School of Pure and Applied Sciences, University of Tsukuba,
Tsukuba, Ibaraki 305-8573

Efficient bidirectional microfluidic transport was achieved through the reversible electrochemical production and shrinkage of hydrogen bubbles. A three-electrode system with a platinum black working electrode, a Ag/AgCl reference electrode, and a platinum auxiliary electrode was incorporated into a poly(dimethylsiloxane) structure with the flow channels and compartments. The influence of the electrode and flow channel structures on the operation of the system was investigated. The production and shrinkage of bubbles was achieved by applying appropriate potentials to the working electrode, which minimized the influence of spontaneous shrinkage resulting from the oxidizing effect of dissolved oxygen. Device performance depended on the structure of the working electrode, meaning that further optimization will be necessary. The device was shown to withdraw solution through a minimally invasive needle and to process liquid plugs in a microfluidic system.

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20.

未反応ラベル酵素の活性に基づく
シングルステップ電気化学イムノクロマトグラフィー

兵庫県立大a、パナソニックb

○富永浩平a、有本 聡b、下野 健b、吉岡俊彦a、水谷文雄a、安川智之a

SINGLE-STEP ELECTROCHEMICAL IMMUNOCHROMATOGRAPHY BASED
ON AN ACTIVITY OF UNCAPTURED LABELED ENZYME

KoheiTOMINAGAa, Satoshi ARIMOTOb, Ken SHIMONOb,
Toshihiko YOSHIOKAb, Fumio MIZUTANIa, and TomoyukiYASUKAWAa

aGraduate School of Material Science, University of Hyogo,
3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297
bBio Research Department, Device Research Laboratory,
Advanced Research Division, Panasonic Corporation, Kyoto 619-0237

An electrochemical immunochromatographic device has been developed to detect a target molecule by the simple procedure of the sample loading. A device was based on two pieces of nitrocellulose membrane, a sample pad with glucose oxidase-labeled anti-mouse IgG antibody (GOx labeled antibody), a conjugate pad with glucose and platinum electrode. When a solution containing model target, mouse IgG, was applied to a chamber on an immunochromatography device, mouse IgG was captured to the anti-mouse IgG antibody immobilized on the nitrocellulose membrane together with GOx labeled antibody to form the sandwich structure, while uncapturedGOx labeled antibody arrived at the conjugate pad with glucose. Hydrogen peroxide generated by the enzyme reaction was detected at the platinum electrode. Oxidation current of hydrogen peroxide decreased with increasing the initial concentration of mouse IgG due to the decrease of uncapturedGOx labeled antibody. The results demonstrated that the designed immunochromatography system could be applied for the quantitative and selective detection with single-step application of the solution containing the target molecules.

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21.

エタノールガス可視化計測のためのバイオ蛍光式探嗅カメラ(Sniff-cam)

医科歯科大

○飯谷健太、佐藤敏征、岸川知里、當麻浩司、荒川貴博、三林浩二

BIO-FLUOROMETRIC IMAGING SYSTEM “SNIFF-CAM”
FOR ETHANOL IN THE GAS PHASE

Kenta IITANI, Toshiyuki SATO, Chisato KISHIKAWA,
Koji TOMA, Takahiro ARAKAWA and Kohji MITSUBAYASHI

Tokyo Medical and Dental University,
2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062

A bio-fluorometric imaging system (Sniff-cam) exploiting an enzymatic reaction was developed for measurement of gaseous ethanol in exhaled breath. This system was composed of a highly sensitive camera, an ultraviolet-light emitting diode (UV-LED) array sheet and an alcohol dehydrogenase (ADH) immobilized mesh. Reduced nicotinamide adenine dinucleotide (NADH) is produced by the ADH enzymatic reaction, and its concentration is proportional to that of ethanol vapor. Therefore, in the Sniff-cam, the concentration of ethanol vapor is quantitatively visualized with fluorescence intensities emitted from NADH by UV photo-excitation. The improved detection limit of 0.5 ppm and the high-selectivity for ethanol vapor was evaluated. Finally, the Sniff-cam was applied to measure ethanol in exhaled breath after intake of an alcohol beverage, and a change of ethanol concentration over time was assessed.

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22.

パリレン蒸着膜上に成長させたポリマーブラシのガス検知特性

愛媛大

○坂口智紀、松口正信

FUNCTIONALIZATION OF PARYLENE-A FILMS WITH POLYMER BRUSHES
FOR GAS-SENSING APPLICATIONS

Tomonori SAKAGUCHI and Masanobu MATSUGUCHI

Department of Materials Science and Biotechnology, Graduate school of
Science and Engineering, Ehime University
3,Bunkyo-cho,Matsuyama 790-8577

Parylene can be conveniently coated onto various substrates by a chemical-vapor-deposition (CVD) technique. In this study, parylene-A (PA) was coated onto a surface of a quartz resonator, followed by grafting Poly(N-isopropylacrylamide) using surface-initiated atom transfer radical polymerization (SI-ATRP). HCl gas and water vapor adsorption/desorption properties of resulting PNIPAM brushes on PA were investigated by a quartz crystal microbalance (QCM) technique. The adsorption capacity increased with PNIPAM loading, indicating homogeneous adsorption of HCl molecules inside the brushes. The reversibility was insufficient for HCl gas due to the slow desorption rate and partially irreversible adsorption. The present study showed that functionalization of PA with PNIPAM brushes could provide PA surface HCl gas sensitivity. This technique was proved to be a effective method to fabricate novel gas-sensing materials on various substrates.

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23.

酵素の還元反応を用いた高感度な
アセトンガス用バイオスニファ

医科歯科大

○叶 明、簡 伯任、鈴木卓磨、當麻浩司、荒川貴博、三林浩二

ACETONE BIO-SNIFFER WITH HIGHLY SENSITIVITY BASED ON
ENZYME REDUCTIVE REACTION

Ming YE, Po-Jen CHIEN, Takuma SUZUKI, Koji TOMA, Takahiro ARAKAWA
and Kohji MITSUBAYASHI

Tokyo Medical and Dental University,
2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062

A highly sensitive acetone bio-sniffer (biochemical gas sensor) was developed for measuring acetone concentration in exhaled breath based on lipid metabolism. The fiber-optic bio-sniffer system was constructed by attaching a flow-cell with a secondary alcohol dehydrogenase (S-ADH) immobilized membrane onto an NADH fluorometric optical fiber with a UV-LED excitation light source. NADH is consumed by the enzymatic reverse reaction (acetone reduction) of S-ADH by adjusting of pH in buffer solution, and the consumption is proportional to the concentration of acetone in the gas phase. Phosphate buffer which contained NADH was circulated into the flow-cell to supply co-enzyme and rinse the excessive substrates and products from the optode. The change of fluorescent emitted from NADH is analyzed by the photo multitude. The relationship between the acetone concentration and the difference value of fluorescence intensity was identified from 20 to 5300 ppb. This interval included the concentration of acetone in the breath of healthy people and those suffering from disorders of carbohydrate metabolism. Finally, the acetone bio-sniffer was used to measure breath acetone during an exercise tolerance test inducing lipid metabolism. The concentration of acetone in breath was shown to significantly increase after exercise. This gas-phase biosensor would be capable of non-invasive evaluation of human lipid metabolism.

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24.

PNIPAM微粒子膜の簡便な作製方法の検討とそのHClガス検知特性

愛媛大

○多田 愛、松口正信

FABRICATION OF POLY(N-ISOPROPYLACRYLAMIDE) PARTICLES USING
SPRAY-COATING METHOD AND ITS HCl GAS ADSORPTION PROPERTIES

Ai TADA and Masanobu MATSUGUCHI

Department of Materials Science and Biotechnology, Graduate school of
Science and Engineering, Ehime University
3,Bunkyo-cho,Matsuyama 790-8577

As we reported previously, poly(N-isopropylacrylamide) (PNIPAM) nanoparticles demonstrated reversible response toward HCl gas at room temperature [1]. In this study, we examined a way to simplify the fabrication process of PNIPAM nanoparticles. The PNIPAM solution synthesized via atom transfer radical polymerization (ATRP) below LCST was simply spray-coated on to a heated quartz resonator. PNIPAM particles were successfully formed under optimized conditions. Resulting PNIPAM particles exhibited linear relationship between the HCl gas adsorption capacity and the PNIPAM loading, while the value of the recovery was approximately 90% irrespective of the PNIPAM loading. Water vapor adsorption measurements showed that the amount of water adsorption was very large compared with that of HCl gas adsorption capacity.

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25.

スパッタ法で作製した電極を用いる
電流検出型COセンサ

九大I2CNERa、九大院工b

○石原達己a,b、森 俊和b、伊田進太郎a,b

AMPEROMETRIC CO SENSOR USING ELECTRODE MADE BY
SPATTERING METHOD

Tatsumi ISHIHARA,a,b Toshikazu MORIb and Shintaro IDAa,b

aInternational Institute for Carbon Neutral Energy Research, Kyushu University,
Motooka 744, Nishiku, Fukuoka 819-0395
bDepartment of Applied Chemistry, Faculty of Engineering, Kyushu University,
Motooka 744, Nishiku, Fukuoka 819-0395

Amperometric CO sensor using LaGaO3 based oxide, RuO2 and In2O3-SnO2 as electrolyte, anode and cathode were studied. Oxygen pumping current was increased upon exposure to CO and so CO at few 100ppm can be detected by change in oxygen pumping current. In this study, CO detection property of the sensor of which electrode was prepared by spattering method for increasing reproducibility. It was found that RuO2 and In2O3-SnO2 electrode can be prepared by dc spattering method and the sensor shows reasonable high sensitivity. Therefore, this study reveals that not only powder but also spattered film can be used for electrode of ameprometric CO sensor.

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26.

YSZにグラファイト検知極を付加した
固体電気化学式NO2センサ

九大a、九大産学連携センターb

○藤井利輝a、アンガライニ スリ アユb、池田 弘b、三浦則雄b

ISOLID-STATE ELECTROCHEMICAL NO2 SENSOR
 USING YSZ AND GRAPHITE SENSING-ELECTRODE

Toshiki FUJIIa, Sri Ayu ANGGRAINIb, Hiroshi IKEDAb and Norio MIURAb

aInterdisciplinary Graduate School of Engineering Sciences, Kyushu University,
Kasuga-shi, Fukuoka 816-8580
bArt, Science and Technology Center for Cooperative Research, Kyushu University,
Kasuga-shi, Fukuoka 816-8580

Various carbon materials, such as graphite, activated carbon, carbon black, graphene and carbon nanotube, were examined for a sensing-electrode (SE) material used in potentiometric yttria-stabilized zirconia (YSZ)-based gas sensors. The SE and the reference-electrode (RE) were formed by applying each of carbon-material pastes and a Pt paste, on the outer and inner surface of a YSZ tube, respectively, followed by heat-treatment in air. The difference in potential (emf) between the carbon-SE and Pt-RE was measured in a flow (100 cm3/min) of each of eight kinds of gases (100 ppm, diluted with air). It was found that, among the 4carbon-SEs examined, the graphite-SE gave high sensitivity and relatively high selectivity against NO2, even at intermediate temperature of 350ºC. The NO2 sensitivity of the YSZ sensor using the graphite-SE changed almost linearly with the logarithm of NO2 concentration in the examined range of 5–100 ppm. The short-term stability of the sensitivity to 100 ppm NO2 for the preset sensor was examined at 350ºC for 20 days. As a result, the NO2 sensitivity was hardly changed during the test period except for the initial several days. From these results, graphite is considered to be a new SE-material for potentiometric solid-state electrochemical NO2 sensors.

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27.

LSM多孔質球状粒子を用いたNOxセンサ

ファインセラミックスセンターa、長崎大b

○末廣 智a、大川 元a、木村禎一a、高橋誠治a、上田太郎b

CHARACTERIZATION OF NOX SENSOR USING LSM POROUS PARTICLES
SENSING ELECTRODE.

Satoshi Suehiroa, Hajime Okawaa, Teiichi Kimuraa, Seiji Takahashia and Taro Uedab

aJapan fine Ceramics Center,2-4-1 Mutuno, Atsuta-ku, Nagoya, 456-8587
bDivision of Chemistry and Materials Science, Graduate School of Engineering , Nagasaki
University, 1-14 Bunkyo-machi, Nagasaki City, Nagasaki, 852-8521

La0.8Sr0.2MnO3(LSM)perovskite type oxide has much attention for NOx sensor using on-board system. In this study. We synthesized the LSM porous particles and evaluated a sensing properties of NOx sensor using LSM porous sensing electrode. The NOx censor with LSM sensing electrode were prepared by the following process: 1) spray pyrolysis of aqueous solutions of La(NO3)3・6H2O, Sr(NO3)2 , Mn(NO3)2・6H2O and citric acid at 400 oC, 2) carbonization of the powder at 800 oC in N2 gas flow, and finally 3) pasting the powder on zirconia electrolyte substrate and then firing at 1100 oC in air. The utilized LSM perovskite porous particle had a secondary particle diameters from 0.5 to 3 m, and a pore size of under1 m. The NOx gas sensor using LSM porous electrode showed a relative higher NO gas selectivity than conventional LSM spherical particles at 450 oC.

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28.

スピネル型酸化物を検知極に用いた
電流検出型ジルコニアセンサによる選択的NO2検知

九大a、九大産学連携センターb

○吉田宗一郎a、池田 弘b、アンガライニ スリ アユb、三浦則雄b

SELECTIVE NO2 DETECTION BY USING AMPEROMETRIC ZIRCONIA-SENSOR
ATTACHED WITH SPINEL-OXIDE SENSING-ELECTRODE

Soichiro YOSHIDAa, Hiroshi IKEDAb, Sri Ayu ANGGRAINIb, Norio MIURAb

aInterdisciplinary Graduate School of Engineering Sciences, Kyushu University
Kasuga-shi, Fukuoka 816-8580
b Art, Science and Technology Center for Cooperative Research, Kyushu University
Kasuga-shi, Fukuoka 816-8580

We examined here the gas sensing characteristics for amperometric yttria-stabilized zirconia (YSZ)-based sensors using each of several spinel-type oxides as a sensing electrode (SE). The SE and counter electrode (CE) of the sensor were formed by applying a metal-oxide paste and a Pt paste, respectively, on the surface of a YSZ tube, followed by sintering at 1200ºC for 2 h in air. The Pt reference-electrode (RE) was formed on its inner surface of the YSZ tube. It was found that, among the oxide-SEs examined, NiFe2O4-SE gave the highest sensitivity to NO2 and negligible responses to seven kinds of interfering gases at 500ºC. The present sensor showed highly selective and repeatable response to NO2, when the potential of SE was set to -150 mV (vs. Pt-RE). In addition, the electric-current response depended almost logarithmically with the NO2 concentration in the examined range of 5–300 ppm. The sensitivity and selectivity to NO2 for the sensor was confirmed to be almost stable during two weeks tested, except for the initial several days. Thus, it is concluded that the present YSZ-based sensor attached with NiFe2O4-SE can work well at 500ºC in an amperometric mode for selective NO2 detection.

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特別講演

世界初の電池駆動家庭用都市ガス警報器を
実現したガスセンサ技術

大阪ガス

大西久男

GAS SENSOR TECHNOLOGY WHICH HAS REALIZED
THE WORLD’S FIRST BATTERY-DRIVEN CITY GAS ALARM

Hisao OHNISHI

Osaka Gas Co., Ltd., Torishima, Konohana-ku, Osaka 554-0051

We have developed the "ultra-low power micro methane sensor" obtained by laminating nano-columnar structure SnO2 thin film and catalyst layer on the circular diaphragm support layer with a built-in thin film heater. And we have clarified the sensing mechanism and the characteristic variation factor of the "bipolar current detection type electrochemical CO sensor" and developed the self-diagnosis technology of this sensor. In May 2015, Osaka Gas released the world's first battery-driven city gas alarm equipped with these sensors.

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29.

サイズ制御した高分子テンプレートによるガスセンサ材料の多孔質化とそのNO2検知特性

長崎大院工

○石田圭司郎、上田太郎、鎌田 海、兵頭健生、清水康博

NO2 SENSING PROPERTIES OF POROUS GAS-SENSING MATERIALS PREPARED
WITH SIZE-CONTROLLED POLYMER TEMPLATES

Keijiro ISHIDA, Taro UEDA, Kai KAMADA, Takeo HYODO, and Yasuhiro SHIMIZU

Graduate School of Engineering, Nagasaki University
1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

Polymethylmethacrylate (PMMA) microspheres were synthesized in distilled water by ultrasonic-assisted emulsion polymerization. The morphology of PMMA microspheres obtained was relatively uniform and their sizes were controlled within a range of 28 nm to 70 nm by adjusting the additive amount of sodium lauryl sulfate into the emulsion. Porous In2O3 spherical particles (In2O3-p(d), d: the size of the PMMA microsphere (nm) used) were prepared by ultrasonic spray pyrolysis of In(NO3)3 aqueous solution containing the PMMA microspheres. The NO2 response of the In2O3-p(d) sensors, which were fabricated by screen printing, increased with a decrease in the size of the PMMA microspheres at lower temperatures ( 250°C). In addition, reduction in the size of the PMMA microspheres accelerated the response speed of In2O3-p(d) sensors.

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30.

金属標準液から作成した金属添加酸化スズ膜を用いた
半導体式ガスセンサのエタノールに対する感度

愛媛大a、愛媛大産学連携推進センターb、新コスモス電機c

○森 雅美a、定岡芳彦b、上田 剛c、三橋弘和c、中谷幹哉c

SENSITIVITY OF SEMICONDUCTOR GAS SENSORS BASED ON METAL-DOPED
SNO2 FILMS PREPARED FROM MATAL STANDARD SOLUTIONS TO ETHANOL

Masami MORIa, Yoshihiko SADAOKAb, Tsuyoshi UEDAc, Hirokazu MITSUHASHIc, and Mikiya NAKATANIc

aaFaculty of Engineering, Ehime University,
Matsuyama-shi, Ehime 790-8577
bThe Cooperative Center of Scientific and Industrial Research, Ehime University,
Matsuyama-shi, Ehime 790-8577
cNew Cosmos Electric Co. Ltd
Osaka-shi, Osaka 532-0036

We examined the sensing behavior of metal-doped SnO2 semiconductor gas sensors to ethanol. Metal-doped SnO2 films were prepared from mixed solutions of Tin (Sn) and zinc (Zn) or lead (Pb) or titanium (Ti) or molybdenum (Mo) ICP standard solutions. The sensitivity of all fabricated metal-doped SnO2 sensors, Sn/Zn=1, 2, Sn/Pb=1, 2, Ti/(Sn+Ti)=0, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 1.0, Mo/(Sn+Mo)=0.01, 0.1, 0.2, 0.4, to 0.64ppm ethanol were higher than that of metal-doped SnO2 gas sensors prepared from SnO2 powders. The highest sensitivity was observed for the Sn/Zn=1 sensor at 700oC, 0%RH, but the sensitivity of the Zn-doped SnO2 sensor decreased in humidified environment. Scanning electron microscopy (SEM) images show that films prepared from mixed solutions composed of fine particle.

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31.

模擬室内空気下における疾患由来のVOC計測

産総研

○伊藤敏雄、赤松貴文、申 ウソク

MONITORING TO DESEASE-RELATED VOCS IN SIMULATED ROOM AIR

Toshio ITOH, Takafumi AKAMATSU, Woosuck SHIN

National Institute of Advanced Industrial Science and Technology (AIST), Shimo-shidami,
Moriyama-ku, Nagoya 463-8560

We have investigated monitoring of lung cancer-related volatile organic compounds (VOCs) (nonanal, n-decane, and acetoin) and sugar diabetes-related VOCs (acetone and methyl i-butyl ketone) in simulated room air. We have used 300 ?g/m3 of 31 kinds of VOCs as simulated contaminated room air, as this has been proposed to resemble an indoor air-like gas. We have collected sensor signals from a sensor array comprising four commercially available sensors (Figaro TGS 2600, 2602, 2610, and 2620) and our laboratory-developed sensors (Pt, Pd, and Au loaded SnO2 (Pt,Pd,Au/SnO2)). The signals from sensors array have been analyzed using principal component analysis (PCA). It was found that the four TGS sensors were insufficient for discriminating VOC gases, whereas both TGS and Pt,Pd,Au/SnO2 sensors could successfully discriminate between lung cancer- and sugar diabetes-related VOCs in contaminated room air as well as synthetic clean air.

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32.

呼気中微量アセトアルデヒド/エタノール測定器による食道がんリスク判定

京大a、エフアイエスb、日本写真印刷c

青山育雄a、花田真理子b、田中克之b、上坂亜紀b、○丸山 潤c、武藤 学a

RISK ASSESSMENT OF ESOPHAGEAL CANCER USING BREATH
ACETALDEHYDE AND ETHANOL ANALYZER

Ikuo AOYAMAa, Mariko HANADAb, Katsuyuki TANAKAb, Aki UESAKAb,
Jun MARUYAMAc, and Manabu MUTOUa

aDepartment of Therapeutic Oncology, Graduate School of Medicine Kyoto University,
54 kawahara-cho, shogoin, sakyo-ku, Kyoto 606-8507
bNissha Printing Co., Ltd., 3 Mibu Hanai-cho,
Nakagyo-ku, Kyoto 604-8551
cFIS Inc., 3-36-3 Kitazono, Itami, Hyogo 664-0891

Acetaldehyde is produced in the body after drinking alcoholic beverages and is certified as an evident carcinogen of esophagus and head and neck. Acetaldehyde carcinogenicity caused by alcoholic beverages is associated with the genotype of ALDH2 that eliminates acetaldehyde. Thus it is important to research the genotype from the aspect of Preemptive medicine and preventive care. Patch testing and genetic testing are used for identifying the genotype, but patch testing has low accuracy and genetic testing is expensive, time-consuming and having ethical questions. Then we developed an analyzer which can measure breath acetaldehyde and ethanol with high stability, non-invasiveness, and rapidity by applying semiconductor sensor gas chromatograph. Using this analyzer, we developed a method of esophageal cancer risk assessment with high accuracy. The method achieved a sensitivity of 100% and specificity of 92.0% for identifying carriers of inactive ALDH2 genotypes.

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33.

MEMSガスセンサの触媒層に対する
XAFS微小領域解析

富士電機a、大阪ガスb、東京医科歯科大c、北大触媒セd

○村田尚義a、岡村 誠a、岡田夕佳里a、鈴木卓弥a
野中 篤b、大西久男b、和田敬広c、宇尾基弘c、朝倉清高d

XAFS MICRO AREA ANARYSIS FOR MEMS GAS SENSOR

Naoyoshi MURATAa, Makoto OKAMURAa, Yukari OKADAa, Takuya SUZUKIa
Atsushi NONAKAb, Hisao OHNISHIb, Takahiro WADAc, Motohiro Uoc, and Kiyotaka ASAKURAd

aCorporate R & D Headquarters, Fuji Electric Co., Ltd., Hino-shi, Tokyo 191-8502
bEnergy Technology Laboratories, Osaka Gas Co., Ltd., Konohana-ku, Osaka 554-0051
cGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
Bunkyo-ku, Tokyo 113-8549
dCatalysis Research Center, Hokkaido University, Sapporo, Hokkaido 001-0021

We have investigated the chemical state of the PdO in catalyst layer on MEMS-gas sensor by the florescence X-ray absorption fine structure analysis of μm order. We found that the PdO in gas sensor was reduced to Pd metal as the gas selectivity decreased. Based on this result we have modified the form of catalyst layer and achieved a long life time of gas selectivity.

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34.

超低消費電力MEMSガスセンサの
多成分検出に関する研究

富士電機

○岡田夕佳里、岡村 誠、村田尚義、鈴木卓弥

RESEARCH FOR MULTI-COMPONENT DETECTION OF ULTRA LOW POWER MEMS
GAS SENSOR

Yukari OKADA, Makoto OKAMURA, Naoyoshi MURATA, and Takuya SUZUKI

Corporate R & D Headquarters, Fuji Electric Co., Ltd., Hino-shi, Tokyo 191-8502

We have developed the MEMS methane gas sensor which has high sensitivity and selectivity with low power consumption. Recently we tried to expand the application for another gas sensing based on the MEMS processing technique in methane gas sensor. It was found that liquefied petroleum (LP) gas, hydrogen or volatile organic compounds (VOCs) can be detected with lower power consumption than that of methane gas sensor by optimizing the heater temperature and driving condition. Moreover, acetone and ethanol can be detected even in a low concentration less than 1ppm.

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35.

接触燃焼式マイクロ水素センサ

矢崎エナジーシステム

○柴田真紀子、波多野博憲、奥野辰行、名川良春

DEVELOPMENT OF A CATALYTIC COMBUSTION TYPE HYDROGEN GAS SENSOR
USING MEMS TECHNOLOGY

Makiko SHIBATA, Hironori HADANO, Tatsuyuki OKUNO, and
Yoshiharu NAGAWA

Gas Equipment Reserch and Development Center, Yazaki Energy System Corporation.
Hamamatsu, Shizuoka 431-3393

In an action for the realization of the hydrogen society of these days, it is important issue using hydrogen more safely. Especially, a sensor detecting a hydrogen leak quickly and precisely is necessary. The contact combustion-type gas sensor is generally used from the viewpoint of linearity with respect to concentration and superior performance to ambient temperature and humidity. However, the sensor needed the response time to hydrogen from several seconds to several ten seconds for this reason, the significant downsizing of the sensor element was necessary for the high-speed sensor response. Therefore, we developed the hydrogen sensor using the MEMS technology in this study. As a result, the response of the micro-gas sensor could be reduced to 40 msec by micro-miniaturization of the sensor element. In additionally, the durability of the sensor was maintained at the same level as that of the conventional-type sensor by attaching a filter to the micro-gas-sensor in atmospheric conditions with poisons substances such as silicon compounds. The hydrogen selectivity of the sensor was controlled by driving at relative low temperature of 300℃ and attaching filter.

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36.

白金-金属酸化物共担持メソポーラスアルミナを用いた
吸着燃焼式マイクロセンサのVOC応答特性

長崎大院工a、矢崎総業b

○永江和徳a、上田太郎a、中越 修a、笹原隆彦b、田邊秀二a
鎌田 海a、兵頭健生a、清水康博a

VOC-SENSING CHARACTERISTICS OF ADSORPTION/COMBUSTION-TYPE
GAS SENSORS USING MESOPOROUS ALUMINA CO-LOADED WITH PLATINUM AND
METAL OXIDE

Kazunori NAGAEa,Taro UEDAa,Osamu NAKAGOEa,Takahiko SASAHARAb
Shuji TANABEa,Kai KAMADAa,Takeo HYODOa and Yasuhiro SHIMIZUa

aGraduate School of Engineering,Nagasaki University,
1-14 Bunkyo-machi,Nagasaki 852-8521,Japan
bResearch and Technology Center,Yazaki Corporation,
1500 Mishuku,Shizuoka 410-1194,Japan

Sensing properties to volatile organic compounds (VOCs) of adsorption/combustion-type micro gas sensors have been examined at an operation with a mode of pulse-driven heating. Among all the sensing materials tested,mesoporous (mp-)Al2O3 co-loaded with Pt (1.0 wt%) and CeO2 (10 wt%) showed the largest dynamic response to 1000 ppm ethanol,while the co-loading with Pt (1.0 wt%) and Bi2O3 (10 wt%) to mp-Al2O3 largely enhanced the dynamic response to 1000 ppm toluene. The derivative analysis of the dynamic responses indicated that the co-loading with Pt and a suitable metal oxide was effective in controlling the catalytic combustion properties of VOCs as well as the VOC-sensing properties.

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37.

RuO2-Ta2O5触媒上でのリン酸水素イオンの
酸化に対する組成と構造の影響

同志社大院工a,同志社大工b

○津熊崇湖a、盛満正嗣a,b

EFFECTS OF COMPOSITION AND STUCTURE OF RuO2-Ta2O5 CATALYST ON
OXIDATION OF HYDROGEN-PHOSPHATE ION

Takami TSUKUMAa and Masatsugu MORIMITSUa,b

aDepartment of Science of Environment and Mathematical Modeling,
bDepartment of Environmental Systems Science,
Doshisha University
Kyotanabe, Kyoto 610-0394

The oxidation of HPO42- on RuO2-Ta2O5 catalytic coatings prepared by thermal decomposition was examined to develop a novel detecting electrode of an electrochemical phosphate ion sensor. Thermal decomposition of a precursor solution containing Ru(III) and Ta(V) was carried out at various metal compositions and temperatures to prepare the detecting electrode. Cyclic voltammetry and chronoamperometry were performed with the obtained electrodes to examine the oxidation behaviors and the concentration dependence of the diffusion-limited current of HPO42-. The oxidation started at ca. 0.8 V vs. Ag/AgCl and the current density was linear to the concentration of HPO42-, in which the amorphous RuO2-Ta2O5 catalyst prepared at 260 oC showed a higher sensitivity than the crystalline one obtained at 500 oC. The sensitivity to HPO42- also depended on the metal composition, and the amorphous catalyst at 50 mol% Ru presented a maximum sensitivity. It was further revealed that the sensitivity of the amorphous catalyst was unchanged in a wide range of HPO42- concentration from 10-3 mmol/L to 10 mmol/L.

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38.

オイル中の酸性度評価用センサの開発

長崎大院工a、長崎大工b

○兵頭健生a、湯藤正篤b、谷川博亮b、上田太郎a
鎌田  海a、清水康博a

DEVELOPMENT OF SOLID-STATE SENSORS MONITORING ACIDITY IN OILS

Takeo HYODOa, Masahiro YUTOb, Hiroaki TANIGAWAb, Taro UEDAa, Kai KAMADAa,
and Yasuhiro SHIMIZUa

aGraduate School of Engineering, bSchool of Engineering, Nagasaki University
1-14 Bunkyo-machi, Nagasaki 852-8521

The deterioration of machine lubricants and engine oils can be commonly estimated through total acid number (TAN), which is the acidity measured by a general titration using KOH(aq.). However, its operation is quite complicated and thus online monitoring the TAN is quite difficult. Therefore, development of solid-state sensors using ion-sensitive field-effect transistor (IS-FET) has been attempted in this study. Both the IS-FET and a reference Ag/AgCl electrode, which were set at the tip surface of general pH sensors, were coated with a cation-conducting polymer film. The sensors obtained were capable of monitoring the acidity of both as-received and heat-treated oils, and the pH values, which were directly measured by the sensors, were relatively correlated with the derived pH values, which were calculated from TAN measured by the general titration method.

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39.

インサーション材料を用いた全固体型
ナトリウムイオン選択性電極の特性評価

東京理科大

○大浦滉平、山際清史、駒場慎一

ELECTROCHEMICAL CHARACTERIZATION OF ALL-SOLID-STATE SODIUM
ION-SELECTIVE ELECTRODES WITH INSERTION MATERIALS

Kohei OHURA, Kiyofumi YAMAGIWA, and Shinichi KOMABA

Department of Applied Chemistry, Tokyo University of Science,
1-3 Kagurazaka, Shinjuku,
Tokyo 162-8601

Our group has developed all-solid-state Na ion-selective electrodes with electroactive Na insertion materials as internal mediating layer sandwiched with electrode substrate and ion selective membrane (ISM). In our previous work, various Na insertion materials, which are known as candidate materials for positive electrode of Na ion batteries, were employed as the mediation layer. The double-layer electrodes of Pt/(insertion material)/ISM showed much better potential stability than that of the Pt/ISM electrode (i.e., direct-contact electrode; DCE). Among them, Pt/Na0.33MnO2/ISM demonstrated the best Na ion-sensing performance, such as sensitivity, response speed, and potential stability. In this study, effects of the membrane thickness and loading of insertion material were examined to improve the electrochemical performances of the Pt/Na0.33MnO2/ISM. Electrode impedance was effectively reduced by optimizing the loading of the insertion material and preparing thinner ion selective membrane, leading to superior electrode performance. Detailed mechanism of the effects will be further discussed.

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40.

ジピコリルアミン-亜鉛(II)錯体を修飾した延長ゲート型有機
トランジスタによるリン酸イオン類の検出

山形大a、山形大ROELb

○南木 創a,b、南 豪a,b、時任静士a,b

DETECTION OF PHOSPHATES USING AN EXTENDED-GATE TYPE ORGANIC
TRANSISTOR FUNCTIONALIZED WITH A ZNII-DIPICOLYLAMINE COMPLEX

Tsukuru MINAMIKIa,b, Tsuyoshi MINAMIa,b, and Shizuo TOKITOa,b

a,bGraduate School of Science and Engineering, Research Center for Organic Electronics,
Yamagata University,
Yonezawa, Yamagata 992-8510

We herein report on the development of a phosphate sensor based on an extended-gate type organic field-effect transistor (OFET). The fabricated OFET possesses a detection part functionalized by a Zn(II)-dipicolylamine derivative, which shows a cross-reactive response to phosphate anions. In addition, we have succeeded in detecting a phosphoprotein (=α-casein) using the fabricated OFET sensor. These results suggest that the ultra-low cost and simultaneous detection of organic and inorganic phosphates and phosphoproteins by OFET sensors is potentially achieved in the near future.

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